Resistance monitoring of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to risk-reduced insecticides and cross resistance to spinetoram

Neto, Jaconias Escócio Lima; Amaral, Marcelo H.; Siqueira, Herbert A.A.; Barros, Reginaldo; Silva, Paolo A. F.

doi:10.1007/s12600-016-0553-y

Cited by 33 publications

(11 citation statements)

References 27 publications

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“…As observed by Xia et al (2014) in China, with the report of three or even four insecticide applications per week against P. xylostella, similar practices have been described in some Brazilian Brassica plantations of the Federal District and in Pernambuco State AMARAL, 2002). Several areas of production have been also abandoned due to the control failure and inefficiency of insecticides leading farmers to use (without success) up to 4 applications per week (OLIVEIRA et al, 2011;LIMA NETO et al, 2016).…”

Section: Introductionmentioning

confidence: 61%

High resistance levels in brazilian Plutella xylostella populations: needs for adjustments in field concentration

et al. 2023

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Plutella xylostella cause severe damage on cruciferous plants all over the world. Farmers in several regions of Brazil report increasing inefficiency of chemical control, even when using high insecticide concentrations. We evaluate here the susceptibility of brazilian diamondback moth populations to the Premio® (Chlorantraniliprole), Dipel® (Bacillus thuringiensis var. kurstaki) and Lannate® BR (Oxime Methylcarbamate) insecticides, frequently used in Brazil. Susceptibility bioassays with five field-collected and two laboratory diamondback moth populations were conducted with increasing concentrations of insecticides up to ten times above the recommended concentration. Extremely high and region-dependent resistances were found in field populations against Chlorantraniliprole and B. thuringiensis, with Resistance Ratios up to 370.0 times for the Camocim de São Felix population. For Oxime Methylcarbamate, we were not able to do Probit analyses for the field populations because of very low mortality rates. Laboratory populations showed resistance to the three tested insecticides with all LC50 concentrations exceeding the recommended doses by at least 3 times. Our results show strong and variable resistance to the three tested insecticides according to the region of origin. To maintain efficient pest control in a large country like Brazil, local levels of resistance need therefore to be monitored by the authorities and reassessment and adjustments of regional doses of insecticides should be implemented as a public policy, to prevent massive spread of insecticides in the field, as well as increases in cases of resistance.

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Section: Introductionmentioning

confidence: 61%

High resistance levels in brazilian Plutella xylostella populations: needs for adjustments in field concentration

et al. 2023

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“…estimation of LC 50 across pest populations), the use of diagnostic concentrations is better suited to detect small increases in resistance when resistant individuals are rare, and logistically easier to implement because fewer individuals per population need to be evaluated (Roush & Miller, 1986;Halliday & Burnham, 1990;Marcon et al, 2000). Diagnostic concentrations were successfully used to track the evolution of resistance to chlorantraniliprole and spinosad in P. xylostella (Wang & Wu, 2012;Neto et al, 2016), diamides and Cry1Ac in H. armigera (Zhang et al, 2011;Pereira et al, 2020), and Cry1F in S. frugiperda (Chandrasena et al, 2018). The baseline data on susceptibility, diagnostic concentrations, and cross-resistance patterns established here will be useful to develop effective insecticide resistance management strategies to mitigate field-evolved resistance to broflanilide in H. armigera, P. xylostella, and S. frugiperda.…”

Section: Discussionmentioning

confidence: 99%

Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

et al. 2022

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Broflanilide is a novel meta-diamide insecticide that acts as a γ -aminobutyric acid-gated chloride channel allosteric modulator. With its unique mode of action, broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance. Establishing the baseline susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies. Here we evaluated the baseline susceptibility to broflanilide for 3 cosmopolitan lepidopteran pest species, Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda. Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China, with median lethal concentrations (LC 50 ) ranging between 0.209 and 0.684, 0.076 and 0.336, and 0.075 and 0.219 mg/L for H. armigera, P. xylostella, and S. frugiperda, respectively. Amongpopulation variability in susceptibility to broflanilide was moderate for H. armigera (3.3fold), P. xylostella (4.4-fold), and S. frugiperda (2.9-fold). The recommended diagnostic concentrations for H. armigera, P. xylostella, and S. frugiperda were 8, 4, and 2 mg/L, respectively. Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P. xylostella and 1 spinosyns-resistant strain of S. frugiperda. Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.

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“…Currently, the control of P. xylostella is heavily dependent on pesticides causing several environmental problems including health issues and pollution and the development of resistance of the target insects ( Pu et al, 2010 ; Yin et al, 2019 ). To the best of our knowledge, P. xylostella has developed resistance to over 95 insecticide compounds ( Guo et al, 2013 ; Liu et al, 2015 ; Lima Neto et al, 2016 ). Although chlorantraniliprole (CL) is a novel diamide insecticide that is effective for control of P. xylostella ( Guo et al, 2014 ), a field population of DBM has developed a 2,000-fold resistance to CL after 2 years of exposure in Southern China and Southeast Asia ( Edralin et al, 2011 ; Wang and Wu, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Bacillus thuringiensis and Chlorantraniliprole Trigger the Expression of Detoxification-Related Genes in the Larval Midgut of Plutella xylostella

Shabbir

Yang²,

Batool

et al. 2021

Front. Physiol.

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Background: Diamondback moth (DBM), Plutella xylostella (L.), has developed resistance to many insecticides. The molecular mechanism of DBM resistance to Bt-G033A combined with chlorantraniliprole (CL) remains undefined.Methods: In this study, field-resistant strains of Plutella xylostella to three pesticides, namely, Bacillus thuringiensis (Bt) toxin (Bt-G033A), CL, and a mixture of Bt + CL, were selected to evaluate the resistance level. Additionally, transcriptomic profiles of a susceptible (SS-DBM), field-resistant (FOH-DBM), Bt-resistant (Bt-DBM), CL-resistant (CL-DBM), and Bt + CL-resistant (BtC-DBM) strains were performed by comparative analysis to identify genes responsible for detoxification.Results: The Bt-G033A was the most toxic chemical to all the DBM strains among the three insecticides. The comparative analysis identified 25,518 differentially expressed genes (DEGs) between pairs/combinations of strains. DEGs were enriched in pathways related to metabolic and catalytic activity and ABC transporter in resistant strains. In total, 17 metabolic resistance-related candidate genes were identified in resistance to Bt-G033A, CL, and Bt + CL by co-expression network analysis. Within candidate genes, the majority was upregulated in key genes including cytochrome P450, glutathione S-transferase (GST), carboxylesterase, and acetylcholinesterase in CL- and BtC-resistant strains. Furthermore, aminopeptidase N (APN), alkaline phosphatase (ALP), cadherin, trypsin, and ABC transporter genes were eminent as Bt-resistance-related genes. Expression patterns of key genes by the quantitative real-time PCR (qRT-PCR) proved the credibility of transcriptome data and suggest their association in the detoxification process.Conclusion: To date, this study is the most comprehensive research presenting functional transcriptome analysis of DBM using Bt-G033A and CL combined insecticidal activity.

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Resistance monitoring of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to risk-reduced insecticides and cross resistance to spinetoram

Cited by 33 publications

References 27 publications

High resistance levels in brazilian Plutella xylostella populations: needs for adjustments in field concentration

High resistance levels in brazilian Plutella xylostella populations: needs for adjustments in field concentration

Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

Bacillus thuringiensis and Chlorantraniliprole Trigger the Expression of Detoxification-Related Genes in the Larval Midgut of Plutella xylostella

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