Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Tomat, David Damián; Balagué, Claudia; Casabonne, Cecilia; Verdini, Roxana Andrea; Quiberoni, Andrea del Luján

doi:10.1016/j.ifset.2015.04.010

Cited by 7 publications

(10 citation statements)

References 45 publications

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“…More vigorous thermal treatments (70, 80, and 90°C) resulted in a complete inactivation of the phages evaluated. Our previous work on coliphages showed a similar phage activity at 50°C to the phages analyzed here (Tomat et al, 2015) and to other coliphages (Coffey et al, 2011). However, assays related to Shigella phage activity had never been previously carried out at these temperatures.…”

Section: Resultssupporting

confidence: 84%

“…As for other phages, significant reductions were observed for two coliphages after treatments of 70 or 80°C (Coffey et al, 2011). Regarding the influence of higher temperatures (i.e., 80 and 90°C) on phage viability, populations of Shi30, Shi33, and Shi93 were below the detection limit (< 10 PFU ml −1 ) after a 1‐h incubation as it was also found for other S. flexneri phages (Lee et al, 2016; Mallick et al, 2021; Shahin & Bouzari, 2018; Zhang et al, 2012) and for other phages (Tomat et al, 2015; Zhang et al, 2013).…”

Section: Resultsmentioning

confidence: 65%

“…These steps were repeated three times (phage stock). High-titer phage suspensions were prepared as previously described (Tomat et al, 2015) using TSB medium and the ATCC 12022 strain, subsequently enumerated (plaque formation units per milliliter; PFU ml −1 ) (Jamalludeen et al, 2007) and stored at 4°C.…”

Section: Bacterial Strains and Phagesmentioning

confidence: 99%

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Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Tomat

González

Aquili

et al. 2022

Food Processing Preservation

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Ten bacteriophages with lytic activity against Shigella flexneri were tested for their resistance to physicochemical conditions found in food matrices. Phage viability and activity were evaluated at different temperatures, pH values, and NaCl concentrations.In addition, challenge tests with each individual phage against ATCC 12022 were conducted to evaluate their use as biocontrol agents. Although phages showed high resistance up to 60°C, Shi30, Shi33, and Shi93 showed higher thermal resistance (70°C).The ten phages endured pH treatments, being more resistant to alkaline conditions. The NaCl concentrations evaluated showed no significant influence on phage counts. Furthermore, challenge tests against ATCC 12022 resulted in significant CFU ml −1 reductions. The results showed that the phages evaluated were resistant to a wide range of temperatures and pH and to all the concentrations of NaCl tested. Therefore, the phages evaluated in this study could potentially be used with biocontrol purposes in various food matrices prior further characterization.Novelty impact statement: Ten newly isolated phages against the foodborne pathogen Shigella flexneri remained viable after different physicochemical treatments. Furthermore, their activity was maintained after thermal and chemical treatments. Also, the ten phages were effective against pathogenic Shigella flexneri strains in challenge tests.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 65%

See 1 more Smart Citation

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Tomat

González

Aquili

et al. 2022

Food Processing Preservation

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“…The alkaline chloride foam (biocide C) and ethoxylated nonylphenol + phosphoric acid (biocide E) biocides at 2.5% v/v (pH >12; 25 °C) and 0.8% v/v (pH <2; 40 °C), respectively, reduced phage counts below the detection limit after 2 min (data not shown). Likely the extreme pH of the treatment solutions was responsible for this inactivation as previously observed (Tomat et al ., ). Pujato et al .…”

Section: Resultsmentioning

confidence: 97%

Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings

Tomat

Balagué

Aquili

et al. 2017

Int J of Food Sci Tech

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Summary Phages are potentially useful as antimicrobial agents in food‐processing environments, provided they can remain active upon extended storage and in the presence of sanitisers. Survival of six phages lytic against enteropathogenic (EPEC) and shiga‐toxigenic (STEC) Escherichia coli strains was assessed upon storage at 4 °C, −20 °C and −70 °C in phosphate‐buffered‐saline (PBS) and Tris‐magnesium‐gelatine buffer (TMG) for up to 1 year. The phages were also exposed to ethanol, sodium hypochlorite, peracetic acid, quaternary ammonium chloride (biocide A), hydrogen peroxide/peracetic acid/peroctanoic acid (biocide B), p‐toluensulfonchloroamide (biocide D) and alkaline chloride foam (biocide C). Plaque‐forming units remained unchanged when the phages were stored at 4 °C in both buffers tested, but decreased by 3.5 and 5.7 log10 PFU when stored in PBS at −20 and −70 °C, respectively. Moreover, TMG seems to be the most protective suspension medium with decreasing temperature because a 1−log10 PFU reduction was observed at −20 and −70 °C. Incubation in 100% ethanol for 24 h reduced plaque counts only by 2.5 log10 PFU. In 10 ppm of sodium hypochlorite and in biocide B (0.13%), the counts decreased by ~5 and ~6 log10 PFU after 15 min. Finally, biocide A and D reduced counts by 4 and 2–4 log10 PFU after 30 min and 8 h of incubation, respectively. Phages were completely inactivated only by peracetic acid and biocides C and E. Therefore, the phages evaluated might be potentially applied together with classical sanitisers such as ethanol and industrial biocides A, B and D, in disinfection programs against pathogenic STEC and EPEC strains.

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“…They are generally found in the gut and share similar morphologies, sizes, structures and resistance against disinfection methods with enteric viruses [177,178]. For example, coliphages can be resistant to environmental stresses, such as hydrostatic pressure (less than 1−log 10 reduction at 600 MPa for 1 h), thermal inactivation (4−log 10 reduction at 72 • C for 20 min) and high or low pHs (no significant log 10 reductions between pH 4 to 11 for 3 h) [179,180]. Therefore, coliphages can act as indicators for enteric viruses and human fecal pollution in rivers, drinking water and underground water [181].…”

Section: Coliphagementioning

confidence: 99%

Biological Indicators for Fecal Pollution Detection and Source Tracking: A Review

Saleem

Edge

et al. 2021

Processes

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Fecal pollution, commonly detected in untreated or less treated sewage, is associated with health risks (e.g., waterborne diseases and antibiotic resistance dissemination), ecological issues (e.g., release of harmful gases in fecal sludge composting, proliferative bacterial/algal growth due to high nutrient loads) and economy losses (e.g., reduced aqua farm harvesting). Therefore, the discharge of untreated domestic sewage to the environment and its agricultural reuse are growing concerns. The goals of fecal pollution detection include fecal waste source tracking and identifying the presence of pathogens, therefore assessing potential health risks. This review summarizes available biological fecal indicators focusing on host specificity, degree of association with fecal pollution, environmental persistence, and quantification methods in fecal pollution assessment. The development of practical tools is a crucial requirement for the implementation of mitigation strategies that may help confine the types of host-specific pathogens and determine the source control point, such as sourcing fecal wastes from point sources and nonpoint sources. Emerging multidisciplinary bacterial enumeration platforms are also discussed, including individual working mechanisms, applications, advantages, and limitations.

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Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

Cited by 7 publications

References 45 publications

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Resistance of phages lytic to pathogenic Escherichia coli to sanitisers used by the food industry and in home settings

Biological Indicators for Fecal Pollution Detection and Source Tracking: A Review

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