Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Abstract

BACKGROUNDThe use of spinosyn insecticides is one of the major control strategies of the fall armyworm, Spodoptera frugiperda (J. E. Smith) in Brazil. In this study, we selected a spinetoram‐resistant strain from a field‐population of S. frugiperda to characterize the inheritance of the resistance and cross‐resistance relationship between spinosyn insecticides.RESULTSThe values of LC50 (95% CI) obtained from concentration–response bioassays were 0.63 (0.55–0.73) μg spinetoram mL−1 for the susceptible strain (SUS), and 1170.96 (1041.61–1323.89) μg spinetoram mL−1 for the strain resistant to spinetoram (SPT‐R). These values resulted in a resistance ratio of 1844‐fold. The SPT‐R strain showed cross‐resistance with spinosad (resistance ratio = 1196‐fold). The reciprocal crosses showed LC50 values of 3.91 (2.97–5.84) and 5.37 (4.52–6.52) μg spinetoram mL−1, suggesting that the resistance of S. frugiperda to spinetoram is autosomal and incompletely recessive. The backcrosses of the F1 progeny with the SPT‐R strain suggest a resistance with polygenic effect. Estimates of the effective number of loci with equal contributions to the resistance effect were from 1.18 to 1.76, suggesting that resistance to spinetoram is associated with a few genes.CONCLUSIONThe inheritance pattern of resistance of S. frugiperda to spinetoram was characterized as autosomal, incompletely recessive and polygenic. Cross‐resistance between spinosyns was confirmed in S. frugiperda. The importance of this information for implementing insect resistance management strategies is discussed in this paper. © 2020 Society of Chemical Industry

Monitoring of Target-Site Mutations Conferring Insecticide Resistance in Spodoptera frugiperda

“…As a result of frequent insecticide applications, multiple resistance cases have been described for field populations in regions where FAW is native [41,42]. In Brazil and Puerto Rico, for instance, resistance has been reported to pyrethroids, organophosphates, carbamates, spinosyns, benzoylureas and, most recently, diamides [29,[42][43][44][45][46][47]. The genetic inheritance of insecticide resistance in FAW has been investigated, and cases of FAW resistance were described as polygenic and metabolic [46][47][48].…”

There is more than chitin synthase in insect resistance to benzoylureas: Molecular markers associated with teflubenzuron resistance inSpodoptera frugiperda

“…The pattern of genetic inheritance of S. frugiperda resistance to teflubenzuron is similar and common to lepidopteran species resistant to several insecticides and Bt toxins, e.g., Dipel resistance and Cry1Ab resistance in Ostrinia nubilalis (Crambidae) (Huang, Buschman, Higgins, & McGaughey, 1999), and in S. frugiperda resistant to lufenuron (Nascimento et al, 2016), spinosad (Okuma et al, 2018), and spinetoram (Lira et al, 2020).…”

“…Insect resistance evolution to insecticides and Bacillus thuringiensis (Bt) -genetically modified-crops is of great concern to biologists, farmers, industry, and government agencies. The strong selection pressure impinged both by numerous insecticides sprays and the wide adoption of Bt -crops increased resistance frequency in many agroecosystems, especially in the successive crop systems used in the central Cerrado savanna region in Brazil (Bolzan et al, 2019; Carvalho, Omoto, Field, Williamson, & Bass, 2013; Farias, Horikoshi, Santos, & Omoto, 2014; Lira et al, 2020; Okuma et al, 2018; Nascimento, Farias, Bernardi, Horikoshi, & Omoto, 2016).…”

Examination of Acephate Absorption, Transport, Metabolism, and Accumulation in Maize After Root Irrigation for Spodoptera Frugiperda Control

“…Past S.frugiperda control methods included the use of organophosphates (such as methyl parathion) (Renato et al, 2013), carbamates (such as carbaryl) (Gunning & Moores, 2001), and pyrethroids (Gist & Pless, 1985). In recent years, agents such as methylamino abamectin, indoxacarb, chlorantraniliprole, and cyantraniliprole, have been used to control S. frugiperda (Lira et al, 2020). In countries and regions outside China, such as the United States, Mexico, Brazil, Honduras, Jamaica, and Puerto Rico, S. frugiperda has developed signi cant resistance to more than 20 insecticides, including lambda cypermethrin, chlorpyrifos, spinosyn, and lufenuron (Diezroux & Omoto, 2001; Yu, Nguyen, & Abo-Elghar, 2003).…”