Resistance of Kansas <i>Sclerotinia homoeocarpa</i> Isolates to Thiophanate-Methyl and Determination of Associated β-Tubulin Mutation

Ostrander, Jesse; Todd, Richard B.; Kennelly, Megan M.

doi:10.1094/php-rs-13-0120

Cited by 9 publications

(3 citation statements)

References 29 publications

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“…Multiple fungicides from four different chemical classes, including benzimidazole carbamate (MBC), dicarboximide, sterol demethylation inhibitor (DMI) and succinate dehydrogenase inhibitor (SDHI) fungicides, have been developed and marketed for dollar spot control [5]. However, repeated fungicidal applications select for isolates of Clarireedia species with reduced sensitivity to fungicides with differing modes of actions (MOAs) [5][6][7][8][9][10][11]. It is necessary to monitor the development of fungicide sensitivity in Clarireedia species, which is important for subsequent fungicide applications for dollar spot control.…”

Section: Introductionmentioning

confidence: 99%

Global Distributions of Clarireedia Species and Their In Vitro Sensitivity Profiles to Fungicides

Zhang

Dong

et al. 2021

Agronomy

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Dollar spot is reported to be caused by multiple Clarireedia species and is a serious problem on many turfgrasses around the world. To our knowledge, the distribution of different Clarireedia species and their sensitivity profiles to fungicides remains unknown. In this study, a total of 275 isolates were characterized by ITS sequence. Amounts of 124, 59 and 75 isolates were identified as C. jacksonii, C. monteithiana and C. paspali, respectively, while each species of C. homoeocarpa and C. bennettii had only five isolates. Four and three isolates were identified as two potential new species, which remained to be further characterized. C. jacksonii and C. monteithiana were distributed worldwide, while C. paspali was restricted to China. Of the isolates with host information, 81% (93/115) and 19% (22/115) of C. jacksonii isolates were collected from C3 and C4 plants, respectively, 97% (56/58) of the C. monteithiana isolates were collected from C4 plants and all C. paspali isolates were collected from C4 plants. The coexistence of different Clarireedia species on the same C4 host type in the same locales was found in Shanghai (Paspalum vaginatum), Jiangsu (Paspalum vaginatum) and Florida (Cynodon dactylon). The study revealed that differential fungicide sensitivity patterns were observed in different species in Clarireedia for the first time. Similar differential sensitivity profiles were also found in the locales with coexistence of at least two species. The findings from this study suggest that the adjacent coexistence of different Clarireedia species and the differential fungicide sensitivity profiles of different species will complicate dollar spot disease control.

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Section: Introductionmentioning

confidence: 99%

Global Distributions of Clarireedia Species and Their In Vitro Sensitivity Profiles to Fungicides

Zhang

Dong

et al. 2021

Agronomy

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“…gene confers MBC fungicide resistance (Ostrander et al, 2014) and overexpression of either cytochrome P450-dependent sterol 14ademethylase (Cyp51) gene or gain-of-function mutation in the ShXDR1 were responsible for DMI fungicide resistance to Clarireedia spp. (Ma and Tredway, 2013;Sang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Detection, characterization, and management of plant pathogens

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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“…gene confers MBC fungicide resistance (Ostrander et al, 2014) and overexpression either cytochrome P450-dependent sterol 14ademethylase (Cyp51) gene or gain-of-function mutation in the ShXDR1 were responsible for DMI fungicide resistance to Clarireedia spp. (Ma and Tredway, 2013;Sang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of Clarireedia spp. to benzimidazoles and dimethyl inhibitors fungicides and efficacy of biofungicides on dollar spot of warm season turfgrass

et al. 2023

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Dollar spot caused by Clarireedia spp. (formerly Sclerotinia homoeocarpa) is an economically destructive fungal disease of turfgrass that can significantly compromise turf quality, playability, and aesthetic value. Fungicides are frequently used to manage the disease but are costly and potentially unfavorable to the environment. Repeated use of some active ingredients has resulted in reduced efficacy on C. jacksonii causing dollar spot in cool-season turfgrasses in the US. Experiments were conducted to study fungicide sensitivity of Clarireedia spp. as well as to develop alternatives to fungicides against dollar spot on warm-season turfgrass in Georgia. First, 79 isolates of Clarireedia spp. collected across the state were tested on fungicide-amended agar plates for their sensitivity to thiophanate-methyl (benzimidazole) and propiconazole (dimethyl inhibitor). Seventy-seven isolates (97.5%) were sensitive (0.001 to 0.654 μg/mL) and two isolates (2.5%) were found resistant (>1000 μg/mL) to thiophanate-methyl. However, in the case of propiconazole, 27 isolates (34.2%) were sensitive (0.005 to 0.098 μg/mL) while 52 isolates (65.8%) were resistant (0.101 to 3.820 μg/mL). Next, the efficacy of three bio- and six synthetic fungicides and ten different combinations were tested in vitro against C. monteithiana. Seven bio- and synthetic fungicide spray programs comprising Bacillus subtilis QST713 and propiconazole were further tested, either alone or in a tank mix in a reduced rate, on dollar spot infected bermudagrass ‘TifTuf’ in growth chamber and field environments. These fungicides were selected as they were found to significantly reduce pathogen growth up to 100% on in vitro assays. The most effective spray program in growth chamber assays was 100% B. subtilis QST713 in rotation with 75% B. subtilis QST713 + 25% propiconazole tank mix applied every 14 days. However, the stand-alone application of the biofungicide B. subtilis QST713 every seven days was an effective alternative and equally efficacious as propiconazole, suppressing dollar spot severity and AUDPC up to 75%, while resulting in acceptable turf quality (>7.0) in field experiments. Our study suggests that increased resistance of Clarireedia spp. to benzimidazoles and dimethyl inhibitors warrants continuous surveillance and that biofungicides hold promise to complement synthetic fungicides in an efficacious and environmentally friendly disease management program.

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Resistance of Kansas Sclerotinia homoeocarpa Isolates to Thiophanate-Methyl and Determination of Associated β-Tubulin Mutation

Cited by 9 publications

References 29 publications

Global Distributions of Clarireedia Species and Their In Vitro Sensitivity Profiles to Fungicides

Global Distributions of Clarireedia Species and Their In Vitro Sensitivity Profiles to Fungicides

Detection, characterization, and management of plant pathogens

Sensitivity of Clarireedia spp. to benzimidazoles and dimethyl inhibitors fungicides and efficacy of biofungicides on dollar spot of warm season turfgrass

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