The article presents the results of field studies of the collection gene pool of spring barley in the conditions of the Ryazan branch of the Federal State Budgetary Scientific Institution "Federal Scientific Agroengineering Center VIM" in 2017-2021 against the natural background of the development of fungal diseases. The aim of the research was to carry out immunological clustering of the collection gene pool of spring barley in the conditions of the Ryazan region and to identify the most promising forms for inclusion in crosses as parental forms. It was revealed that the vast majority of the studied varieties belonged to the group of highly resistant varieties. It was found that more than half of the varieties (57.8%) of the studied collection possessed complex resistance to a group of pathogens (reticulated (Perenophora teres Drechs), dark brown mottling (Bipolaris sorokiniana Shoem), powdery mildew (Erisiphe gramminis D.C.f.sp. hordei Marchal), dusty smut (Usnilago nuda Jen.)). spring barley. The following varieties showed high complex stability: Nur, Reliable, Moskovsky 3, Moskovsky 2, Dvina, Kalita, Hopper, Clear, Griv, Miar, Risk, Choice (Russia); Timerkhan (Tatarstan); Divosny, Gonor, Yakub, Batka, Fest, Khago (Belarus); Hetman, Kharkiv 102, Vinnytsia 17 (Ukraine); Ilek-1, Ilek-34 (Kazakhstan); Delphin (France); Linga, Irbe (Latvia); Izotta, Graice (Germany); Prosa, Pejas (Czech Republic); Collie (UK); B-934 (Canada). The greatest approximate productivity over the years of research has been revealed in the following varieties: Notable (Russia), Gonar (Belarus), Vivaldi (Austria), Eufel, Pioneer (France), Stratus (Poland), which have an advantage over the Yaromir standard by 0.3 – 0.7 t/ha. Reliable variety (Russia) had the highest resistance to lodging out of the entire studied gene pool of spring barley – 8.6 points. The use of a comprehensive ranking assessment revealed the priority of varieties of the Belarusian selection Divosny, Gonar and Czech varieties Pegas, Prosa, which occupy leading positions on the rating scale in terms of resistance to lodging, disease damage and productivity.