Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method

Abstract

This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed.

Characterization of ESBL producing E. coli from chicken feces by phenotypic methods and MALDI-TOF MS

“…The majority of genes responsible for ESBL appear as CTX-M, SHV, and TEM variants. However, there are also other genes responsible for resistance [39,[56][57][58]. In the study of Laudy et al [59], similar to the present study, although ESBL-producing Pseudomonas aeruginosa strains obtained from phenotypic test results, they could not detect all the genes it screened at the same rate.…”

Faktor Risiko Penyebaran Escherichia coli Penghasil ESBL pada Ternak Ayam Komersial

“…Kasus kejadian Escherichia coli penghasil ESBL pada ternak ayam komersial di Kabupaten Blitar merupakan kasus kejadian Escherichia coli penghasil ESBL yang mengalami resistensi terhadap beberapa antibiotik terutama golongan antibiotik beta lactam (cephalosphorin generasi ketiga dan monobactam) (Wibisono et al, 2020a); (Wibisono et al, 2020b); (Wibisono et al, 2020c); (Wibisono et al, 2020d); (Witaningrum et al, 2020). Hasil penelitian menunjukkan bahwa pada ternak ayam komersial terdapat 94,5% (345/365) positif terdapat Escherichia coli.…”