Resistance profile of urine isolate enterobacteral strains at Donka University teaching hospital in Conakry, Guinea

Abstract

The objective of this study was to describe the resistance profile of enterobacterals isolated from urine samples at the laboratory of Donka National Hospital. Urine samples were collected from both outpatients and hospitalized patients. Cultures were performed using standard techniques, strains were identified using the API 20E kit, and antibiotic susceptibility testing was carried out using the ATB™ UR EU (08) kit. The results were interpreted according to the recommendations of the Antibiogram Committee of the French Society of Microbiology (CASFM v1 2023). Out of a total of 520 urine samples analyzed, 111 were positive for enterobacteral strains. Among them, 75 (67.57%) were of community origin. Escherichia coli was the most represented species (n=61, 55%), followed by Klebsiella pneumoniae (n=24, 22%). The resistance of E. coli strains to third generation cephalosporins (TGC) varied from 5.41% (n=6) to 25.23% (n=28) in the community and from 13 to 38% in the hospital. The profile for carbapenems was categorized as "susceptible to high dosage (SHP)," accounting for 16.22% (n=18). This study provided insight into the resistance profile to antibiotics used in urinary tract infections. The increasing resistance to carbapenems poses a threat to the management of strains producing extended-spectrum beta-lactamases (ESBL). It would be important to strengthen resistance surveillance in this context.