Resistance Testing of Salmonella Spp. Bacteria Isolated from Samples Derived from Poultry on Antimicrobial Drugs

Manić, Marija

doi:10.15406/jdvar.2016.03.00074

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Similar findings (46.87%) were reported by earlier researchers in India [ 43 ]. But higher resistance against colistin (92.68%) was recorded in Bangladesh [ 47 ] and lesser (20%) in Serbia [ 48 ] and (4.76%) in Mumbai, India [ 49 ]. It is beyond the scope of this study to attribute colistin resistance to a particular reason.…”

Section: Discussionmentioning

confidence: 99%

Salmonella from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of Salmonella from Commercial Broiler Supply Chain and Its Environment

Sohail¹,

Rathnamma²,

Priya³

et al. 2021

BioMed Research International

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Antimicrobial resistance (AMR) in poultry production chain is one of the major food safety concerns due to indiscriminate usage of antibiotics and the presence of pathogens such as Salmonella which causes infections in various stages of production. In the present study, 182 samples were collected from commercial broiler supply chain, viz., three hatcheries ( n = 29 ), three commercial broiler farms (CBF; n = 99 ), and three retail meat shops (RMS; n = 54 ), and used for isolation and identification of Salmonella using three different selective agar media and a selective enrichment medium followed by PCR confirmation targeting the hilA gene. The overall prevalence of Salmonella was 47/182 (25.82%), and a significantly higher ( P < 0.05 ) prevalence was observed in retail meat shops (46.29%), CBF (19.19%), and hatcheries (10.34%). Comparison of three agar media for isolation of Salmonella revealed that all the media were equally selective. However, PCR amplification of hilA gene fragment was significantly higher ( P < 0.01 ) in selective enrichment culture tetrathionate brilliant green bile broth (TTB) as compared to all solid (agar-based) media. Susceptibility pattern against most frequently used antibiotics revealed that 100% of the isolates were resistant to at least one antibiotic. High resistance was observed for doxycycline (94.34%), followed by cefpodoxime (84.91%), ciprofloxacin (72.64%), gentamicin (65.09%), enrofloxacin (61.32%), colistin sulphate (40.42%), amikacin (34.91%), ampicillin (33.96%), neomycin (33.02), cefotaxime (30.19%), ceftazidime (29.25%), trimethoprim-sulfamethoxazole (23.58%), amoxicillin+clavulanic acid (21.70%), and chloramphenicol (12.26%); 16.98% of the isolates were ex-tended spectrum β-lactamase (ESBL) producers, and 76.41% were multidrug resistant (MDR). MDR Salmonella were significantly higher ( P < 0.01 ) in RMS (91.66%) followed by CBF (82.75%), whereas no MDR isolates were present in the isolates from hatcheries. The results indicated a higher prevalence of Salmonella and AMR for commonly used antibiotics in the complete broiler supply chain, especially RMS and CBF. Also, this study idicated that TTB enrichment followed by PCR and colony PCR was found to be rapid, specific and time-saving method.

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Section: Discussionmentioning

confidence: 99%

Salmonella from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of Salmonella from Commercial Broiler Supply Chain and Its Environment

Sohail¹,

Rathnamma²,

Priya³

et al. 2021

BioMed Research International

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“…This class of antibiotics offers the advantage of oral administration, high potency against Gram negative organisms and low toxicity (Lee et al, 2004). However, Manic et al (2016) observed that enrofloxacin is not an effective drug in the treatment of salmonellosis of birds since Salmonella colonize the intestine after stoppage of the drug. It was also reported that the use of enrofloxacin can induce resistance in Campylobacter species (Velhner et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

Ejeh¹,

Lawan²,

Abdulsalam³

et al. 2017

Sokoto J. Vet. Sc.

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have been shown to be resistant to multiple antibiotics. We carried out a bacteriological investigation on 105 cloacal swabs from local and broiler chickens retailed along the roadside in Hanwa, Zaria for the occurrence of E. coli and Salmonella spp. by isolation through culture, and identification using biochemical and serotyping techniques. Serotyping of E. coli isolates was carried out with polyvalent E. coli O157:H7 antisera while Salmonella spp. isolates were serotyped using polyvalent antisera specific for all groups and type-factor for Salmonella spp. Presumptive isolates were subjected to antimicrobial susceptibility testing using 13 panels of antibiotics for both E. coli and Salmonella spp. Results showed that the overall isolation rate of Salmonella spp. was 12 (11.4%), broiler chickens had higher isolation rate 9 (12.0%) of Salmonella than local chickens. However, the isolation rate of E. coli from local chickens 15 (50.0%) was higher compared to broilers 6 (8.0%). Also, non-E. coli O157:H7 and E. coli O157:H7 were isolated from both broilers and local chickens at a frequency of 15 (14.3%) and 6 (5.7%) respectively. The overall isolation rate of E. coli was 21 (20.0%). Multiple antibiotic resistance was observed among local and broiler chickens. However, E. coli and Salmonella species were 100% susceptible to Enrofloxacin. We therefore concluded that E. coli and Salmonella species are prevalent in the cloacae of both broiler and local chickens retailed along the roadside in Hanwa, Zaria, Nigeria and could pose serious veterinary and public health risks.

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Performance and Physiological Responses of Salmonella Enteritidis Challenged Broilers Fed Diets Containing Antibiotic, Probiotic and Aromabiotic

Fasanmi¹

2016

JDVAR

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Resistance Testing of Salmonella Spp. Bacteria Isolated from Samples Derived from Poultry on Antimicrobial Drugs

Cited by 3 publications

References 13 publications

Salmonella from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of Salmonella from Commercial Broiler Supply Chain and Its Environment

Salmonella from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of Salmonella from Commercial Broiler Supply Chain and Its Environment

Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

Performance and Physiological Responses of Salmonella Enteritidis Challenged Broilers Fed Diets Containing Antibiotic, Probiotic and Aromabiotic

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