Resistance to a peritoneal lymphoma graft induced by heat-killed S or R Brucella abortus

Dazord, L.; Martin, Anne F.; Amice, J.; Garrec, Y. Le; Toujas, Louis

doi:10.1007/bf00205570

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…Despite the high level of both macrophages and NK cytotoxic activity observed in the peritoneum on day 7 after the injection of bacteria there was no resistance to an IP tumor cell inoculum. In a previous study using killed bacteria, we also demonstrated that the correlation between in vivo resistance to a peritoneal lymphoma inoculum and augmentation of macrophage cytostatic activity was satisfactory with R but not with S strains of Brucella abortus [1]. Thus, while living and inactivated S and R Brucella are able to increase resistance to EL4 lymphoma, the mechanism of the antitumor effect is obviously not yet fully understood.…”

Section: Discussionmentioning

confidence: 71%

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Antitumor activity of living or killed Brucella: Modification of the non-specific cytotoxic effector cells

Dazord

Garrec

Florentin

1983

Cancer Immunol Immunother

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At various times after injection of living or killed smooth (S) or rough (R) Brucella abortus mice received a graft of the semi-allogenic EL4 lymphoma and their survival was studied. In parallel, the NK activity of spleen and peritoneal cells, the level of serum interferon (IFN), and the cytotoxic activity of peritoneal macrophages were investigated. Protection against the lymphoma lasted longer after injection of R organisms than after S. The parallelism between the in vivo resistance to El4 lymphoma and the augmentation of NK and macrophage activity was satisfactory with R but not with S. IFN production did not seem to be correlated with R antitumor activity. The antitumor effect of Brucella cannot therefore be simply explained on the basis of modification of the non-specific cytotoxic effector mechanisms.

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Section: Discussionmentioning

confidence: 71%

“…Some correlation between in vivo resistance to EL4 lymphoma and the presence of highly cytotoxic macrophages in the peritoneal cavity has been observed after injection of rough (R) but not of smooth (S) killed Brucella [1].…”

Section: Introductionmentioning

confidence: 95%

Antitumor activity of living or killed Brucella: Modification of the non-specific cytotoxic effector cells

Dazord

Garrec

Florentin

1983

Cancer Immunol Immunother

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“…The question was raised as to whether the antitumour properties of the immunostimulant-activated macrophages were related to their stage of maturation. We investigated this question, using killed Brueella abortus, an antitumour and immunostimulant agent (Toujas et al, 1972) as a macrophage activator (Dazord et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

Generation of new macrophages after injection of killed Brucella abortus organisms

Amice

Dazord

Toujas

1978

Cancer Immunol Immunother

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Summary. The kinetics of macrophage formation after intravenous injection of inactivated Brucella organisms was investigated in mice. Newly formed macrophages sampled from the peritoneal cavity were identified by their adherence to glass and their recent uptake of tritiated thymidine (~H-thymidine). Radiolabelled cells increased in number from the fifth day after bacterial injection. On the seventh day they were about 20 times as numerous as in the controls. The injection of Brucella also increased the number of Kupffer cells in the liver.The variations in radioactivity in this organ were parallel to those in peritoneal adherent cells.

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Interactions with host macrophages and ability of human melanoma cell lines to grow in nude mice

Benomar¹,

Gerlier²,

Doré³

1986

Intl Journal of Cancer

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The interactions of nude mouse macrophages with five human melanoma cell lines, characterized by their resistance to mouse NK activity and varying in their ability to grow s.c. in nude mice, were investigated. These lines were equally susceptible in vitro to both cytostatic and tumoricidal activities of activated peritoneal macrophages collected from nude mice inoculated 3 days previously with Brucella abortus B19R strains. I.p. injection of a poorly tumorigenic melanoma cell line (PTCL) in nude mice was followed by the local appearance of macrophages able to kill these cells in a 48-hr 3H-thymidine cytotoxicity assay. The level of tumoricidal macrophages was maximum for the first week and then slowly declined to disappear by the 4th week following PTCL inoculation. The use of an HTCL instead of a PTCL also induced macrophages able to kill HTCL cells, but the cytotoxicity level was lower and the activity disappeared more rapidly. In cross-experiments using PTCL-activated macrophages as effectors on HTCL targets, these cells were found to be less sensitive than PTCL cells when macrophages were taken at weeks 2 and 3 following PTCL inoculation. To investigate whether tumoricidal macrophages activated in vivo with human melanoma cells could also act in vivo, we inoculated these s.c. into nude mice, simultaneously with live HTCL cells. Peritoneal cells rich in melanoma-activated macrophages prevented HTCL growth in most recipients, whereas spleen cells from the same donor mice did not modify the tumor take. These data indicate that xenogeneic tumors could activate nude mouse macrophages in vivo and suggest that the ability of human tumors to grow in nude mice could be related to their capacity to activate host macrophages locally and to the susceptibility of human tumor cells to the tumoricidal activity of activated macrophages.

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Resistance to a peritoneal lymphoma graft induced by heat-killed S or R Brucella abortus

Cited by 6 publications

References 9 publications

Antitumor activity of living or killed Brucella: Modification of the non-specific cytotoxic effector cells

Antitumor activity of living or killed Brucella: Modification of the non-specific cytotoxic effector cells

Generation of new macrophages after injection of killed Brucella abortus organisms

Interactions with host macrophages and ability of human melanoma cell lines to grow in nude mice

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