Resistance to Apramycin in Escherichia coli Isolated from Animals: Detection of a Novel Aminoglycoside-modifying Enzyme

Hedges, R. W.; Shannon, Kevin

doi:10.1099/00221287-130-3-473

Cited by 20 publications

(18 citation statements)

References 33 publications

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“…5) was responsible for this coresistance in strains of S. typhimurium and E. coli isolated from both outbreaks. A similar enzyme has been described previously in E. coli strains isolated from animals in Great Britain (10) and in France (2a).…”

Section: Discussionmentioning

confidence: 60%

Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France

Chaslus-Dancla¹,

Martel²,

Carlier³

et al. 1986

Antimicrob Agents Chemother

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We studied two outbreaks of calf salmonellosis caused by apramycin and gentamicin-resistant Salmonella typhimurium strains. In both cases, the responsible strains were resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, tetracycline, and trimethoprim; one strain was also resistant to nalidixic acid in one outbreak. A systematic survey of the intestinal Escherichia coli strains of calves from the two affected flocks showed that 11 of 24 animals sampled were also colonized by apramycin-and gentamicin-resistant E. coli strains. These isolates belonged to four biotypes and were resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, tetracycline, trimethoprim, and nalidixic acid. All of the strains were resistant to high levels of apramycin (MICs, 512 to 1,024 gg/ml) and to gentamicin (MICs, 8 to 32 ,ug/ml), and these resistances were always transferred en bloc. In S. typhimurium, this coresistance was borne by plasmids that were approximately 39 kilobases long (outbreak 1) or 90 kilobases long (outbreak 2), whereas in E. coli, the coresistance was due to plasmids that were approximately 110 kilobases long in both outbreaks. The two plasmids of Salmonella and four plasmids of E. coli encoded type IV aminoglycoside 3-N-acetyltransferases. The intensive use of curative and preventive treatments in calf production could be responsible for the emergence of enzymic resistance to apramycin and gentamicin.Apramycin and gentamicin have recently been introduced into veterinary therapy in France. Although gentamicinresistant strains of Salmonella and Escherichia coli isolated from animals have been reported previously (1,3,7,8,11,17,19), cross-resistance to apramycin has been detected only recently in France (2a).We describe here a study of the genetic basis and the biochemical mechanism of apramycin and gentamicin resistance in strains from two outbreaks of calf salmonellosis. Resistant E. coli strains were also detected in these outbreaks. (colistin, spiramycin, tetracycline, gentamicin, kanamycin, flumequine, oxolinic acid, and nitrofuran) were successively distributed unsuccessfully from day 9 to day 31 after allotment. Of 138 calves, 12 died before day 27. An apramycin-and gentamicin-resistant strain of S. typhimurium, strain BN8700, was isolated in the course of this outbreak. After the end of antibiotic therapy, apramycinand gentamicin-resistant E. coli strains were sought in the feces of 15 calves. MATERIALS AND METHODS(ii) Outbreak 2. Outbreak 2 occurred in October 1984 in a feedlot located in the Rhone-Alpes region among calves which were purchased in England. AMute diarrhea appeared at the end of the first week after allotment. An apramycin-* Corresponding author.and gentamicin-resistant strain of S. typhimurium, strain BN8900, was isolated from the liver of a dead septicemic calf. It was not possible to obtain information on the mortality rate. The only reported antibiotic used in this flock was colistin. Approximately 2 months later, when all of the symptoms had disappeared, apramycin-an...

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Section: Discussionmentioning

confidence: 60%

Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France

Chaslus-Dancla¹,

Martel²,

Carlier³

et al. 1986

Antimicrob Agents Chemother

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“…AAC(1) produces resistance to apramycin, paromomycin, lividomycin, and ribostamycin and has been identified in animal isolates of E. coli (119,169). Probably because AAC(1) enzymes produce no clinically important resistance, the genes for these acetyltransferases have not been cloned, so the distribution of AAC(1) among clinical isolates has not been studied.…”

Section: Aminoglycoside Acetyltransferasesmentioning

confidence: 99%

Versatility of Aminoglycosides and Prospects for Their Future

2003

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Aminoglycoside antibiotics have had a major impact on our ability to treat bacterial infections for the past half century. Whereas the interest in these versatile antibiotics continues to be high, their clinical utility has been compromised by widespread instances of resistance. The multitude of mechanisms of resistance is disconcerting but also illuminates how nature can manifest resistance when bacteria are confronted by antibiotics. This article reviews the most recent knowledge about the mechanisms of aminoglycoside action and the mechanisms of resistance to these antibiotics

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“…Gram-negative bacterial strains resistant to these antibiotics have been isolated from several different animal sources, and the associated resistance mechanisms have been characterized (1,4,9,13). Apramycin resistance is due to N acetylation by an enzyme of the aminoglycoside acetyltransferase 3 class (AAC-3-IV), and hygromycin inactivation is due to 0 phosphorylation by hygromycin B phosphotransferase 4 (HPH-4-I).…”

mentioning

confidence: 99%

Aminoglycoside acetyltransferase 3-IV (aacC4) and hygromycin B 4-I phosphotransferase (hphB) in bacteria isolated from human and animal sources

Salauze

Otal

Gómez-Lus

et al. 1990

Antimicrob Agents Chemother

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Members of the family Enterobacteriaceae harboring an enzyme of the aminoglycoside acetyltransferase 3 class (AAC-3-IV) (apramycin and gentamicin resistance) and hygromycin B phosphotransferase 4 (HIPH-4-I) (hygromycin B resistance) have been isolated from human clinical sources in Europe. A cluster of genes containing IS140, aacC4, and hphB was found in these strains. We demonstrate by Southern hybridization that this cluster is identical to the operon found in animals that also contains insertion sequences belonging to the IS6 family. This provides another example of presumptive transfer of antibiotic resistance genes between bacteria of animal and human origin.

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Resistance to Apramycin in Escherichia coli Isolated from Animals: Detection of a Novel Aminoglycoside-modifying Enzyme

Cited by 20 publications

References 33 publications

Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France

Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France

Versatility of Aminoglycosides and Prospects for Their Future

Aminoglycoside acetyltransferase 3-IV (aacC4) and hygromycin B 4-I phosphotransferase (hphB) in bacteria isolated from human and animal sources

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