Resistance to Chloramphenicol and Fusidic Acid

Smith, Arnold L.; Burns, Jane L.

doi:10.1016/b978-0-12-138120-2.50015-0

Cited by 8 publications

(2 citation statements)

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“…Chloramphenicol inhibition. Induction of the enzyme(s) for the dechlorination of 2,4,6-TCP was examined in an experiment using chloramphenicol to inhibit protein synthesis and hence the synthesis of inducible enzymes (20). Chloramphenicol was added from a sterile 0.2% aqueous stock solution.…”

Section: Methodsmentioning

confidence: 99%

Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture

Madsen

Aamand

1992

Appl Environ Microbiol

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The transformation and toxicity of trichlorophenols (TCPs) were studied with a methanogenic enrichment culture derived from sewage sludge. Transformation of TCPs rapidly resumed after heating of the culture at 80°C for 1 h, suggesting that the dechlorinating bacteria are spore-forming anaerobes. 2,4,6-TCP was rapidly dechlorinated via 2,4-dichlorophenol to 4-chlorophenol. During the transformation of 2,4,6-TCP, the most probable number of dechlorinating bacteria increased by 4 orders of magnitude. The most extensive dechlorination was observed in media with complex carbon sources such as yeast extract, peptone, and Casamino Acids, but glucose, galactose, and lactose were also used by the consortium. Experiments using chloramphenicol indicated that the reductive dechlorination of 2,4,6-TCP was regulated by an inducible enzyme system. The highest initial concentration at which dechlorination of 2,4,6-TCP was observed was 400 ,M. 2,4,5-TCP and 3,4,5-TCP were dechlorinated to, respectively, 3,4-dichlorophenol and 3-chlorophenol at initial concentrations of c40 FIM. Toxicity for the acid-producing and methanogenic bacteria in the consortium was a function of chemical structure, as the inhibition of these activities increased from 2,4,6-TCP, via

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