Resistance to Desiccation in Intertidal Barnacles

Vogel, John E.; Shelokov, Alexis

doi:10.1126/science.126.3269.358-a

Cited by 217 publications

(66 citation statements)

References 3 publications

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“…Each line was passaged 15 to 20 times until growth ceased. Cultures were monitored for CPE; for hemagglutination (9) with rat, rhesus, vervet, human Group 0, guinea pig, and chicken erythrocytes; and for hemadsorption (8) with guinea pig and vervet red blood cells. Supernate and cells were harvested from passages 4, 10, and 15, and inoculated into 2 litters of less than 1-day-old mice and 2 litters of less than 1-day-old hamsters.…”

Section: Methodsmentioning

confidence: 99%

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Viral isolation studies of inclusion positive biopsy from human connective tissue diseases

Feorino

Hierholzer

Norton

1970

Arthritis & Rheumatism

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Section: Methodsmentioning

confidence: 99%

“…Six blind passages covering 3 months of continuous culture were performed. Cultures were monitored for cytopathogenic effects (CPE) and tested for hemadsorption with guinea pig and vervet erythrocytes (8).…”

Section: Methodsmentioning

confidence: 99%

Viral isolation studies of inclusion positive biopsy from human connective tissue diseases

Feorino

Hierholzer

Norton

1970

Arthritis & Rheumatism

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“…The cultures were rolled at 330 C. Some washings were titrated by inoculating 10-fold dilutions made in Hanks's saline. Cultures were examined for the typical syncytial cytopathic effect of para-influenza 2 virus, but the final recording was based on the haemadsorption test (Vogel & Shelokov, 1957); 4-5 days after inoculation, 0-2 ml. of a 100% suspension of human group 'O' red cells in phosphate-buffered saline was added to each tube and the tubes were placed at 40 C. for 30 min.…”

mentioning

confidence: 99%

Para-influenza 2 virus infections in adult volunteers

Taylor‐Robinson¹,

Bynoe²

1963

Epidemiol. Infect.

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Twenty-eight adult volunteers were inoculated intranasally with para influenza 2 virus and eight developed illnesses; twenty-eight volunteers were given flanks' saline and one became ill. The illnesses occurred in volunteers given between 2 × 104 and 2 × 106 TCD 50 of virus. The most prominent symptoms were sore throat, nasal stuffiness and coryza; four of the eight volunteers had sufficient coryza to be regarded as having mild colds. Although only eight volunteers had clinical evidence of infection, twenty-four had laboratory evidence of infection as judged by virus re-isolation or antibody response. Neutralization, haemagglutination-inhibition and complement-fixation tests on paired sera showed that sixteen individuals had a fourfold or greater antibody response by one or more tests including five of the eight volunteers who were ill. Twenty volunteers, including seven who were ill, had reciprocal neutralizing antibody titres of eight or more before inoculation of virus so it seems that the illnesses were due to re infection in the presence of antibody. Evidence is presented which suggests that although illnesses occurred in the presence of antibody they were due to the para influenza 2 virus and not some other agent in the inoculum. The results of these experiments seem to fulifi the third of Koch's postulates for para-influenza 2 virus as a cause of respiratory disease in adults.We wish to thank Dr P. A. J. Tyrreil for his advice during the course of this work and in the preparation of the manuscript. We also thank Dr K. V. Shah (The Johns Hopkins University, Baltimore, U.S.A.) for help in some of the early experiments and Miss B. Ridgwell for valuable technical assistance.

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“…When diagnosing Newcastle disease, some methods were recommended by OIE Manual 23 and EU Council Directive 24 among which include isolation and detection from SPF embryonated chicken eggs, through haemagglutination (HA) inhibition test. Although HA is an old method used in determining the presence of the virus but still being applied in many laboratories of the world for the detection and identification of NDV's titer presence in the samples.…”

Section: Introductionmentioning

confidence: 99%