Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs

López, María José; Rojo–Bezares, Beatriz; Chichón, Gabriela; Sáenz, Yolanda

doi:10.3390/antibiotics11091271

Cited by 7 publications

(4 citation statements)

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“…Commonly, transposons and integrons are localized within plasmids and integrative conjugative elements [65], which coincides with our findings. Notably, the crpP gene was initially identified on a plasmid [58]; however, in this and another study, it was localized in ICEs [66]. Similarly, catB7 was identified in ICEs, which could be related to the loss of this gene in some genomes.…”

Section: Discussionmentioning

confidence: 73%

Comparative Genomics of Pseudomonas aeruginosa Strains Isolated from Different Ecological Niches

et al. 2023

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The Pseudomonas aeruginosa genome can change to adapt to different ecological niches. We compared four genomes from a Mexican hospital and 59 genomes from GenBank from different niches, such as urine, sputum, and environmental. The ST analysis showed that high-risk STs (ST235, ST773, and ST27) were present in the genomes of the three niches from GenBank, and the STs of Mexican genomes (ST167, ST2731, and ST549) differed from the GenBank genomes. Phylogenetic analysis showed that the genomes were clustering according to their ST and not their niche. When analyzing the genomic content, we observed that environmental genomes had genes involved in adapting to the environment not found in the clinics and that their mechanisms of resistance were mutations in antibiotic resistance-related genes. In contrast, clinical genomes from GenBank had resistance genes, in mobile/mobilizable genetic elements in the chromosome, except for the Mexican genomes that carried them mostly in plasmids. This was related to the presence of CRISPR-Cas and anti-CRISPR; however, Mexican strains only had plasmids and CRISPR-Cas. blaOXA-488 (a variant of blaOXA50) with higher activity against carbapenems was more prevalent in sputum genomes. The virulome analysis showed that exoS was most prevalent in the genomes of urinary samples and exoU and pldA in sputum samples. This study provides evidence regarding the genetic variability among P. aeruginosa isolated from different niches.

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Section: Discussionmentioning

confidence: 73%

Comparative Genomics of Pseudomonas aeruginosa Strains Isolated from Different Ecological Niches

et al. 2023

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“…Although nalD frameshifts have been reported previously in fluoroquinolone-resistant isolates in Spain, these specific frameshift mutations are novel. 19 …”

Section: Resultsmentioning

confidence: 99%

Comparative molecular profiling of multidrug-resistant Pseudomonas aeruginosa identifies novel mutations in regional clinical isolates from South India

Menon,

Somanath,

Jossart

et al. 2023

JAC-Antimicrobial Resistance

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Objectives We sought to analyse the antibiotic susceptibility profiles and molecular epidemiology of MDR clinical Pseudomonas aeruginosa isolates from South India using non-MDR isolates as a reference. Methods We established a comprehensive clinical strain library consisting of 58 isolates collected from patients across the South Indian state of Kerala from March 2017 to July 2019. The strains were subject to antibiotic susceptibility testing, modified carbapenem inactivation method assay for carbapenemase production, PCR sequencing, comparative sequence analysis and quantitative PCR of MDR determinants associated with antibiotic efflux pump systems, fluoroquinolone resistance and carbapenem resistance. We performed in silico modelling of MDR-specific SNPs. Results Of our collection of South Indian P. aeruginosa clinical isolates, 74.1% were MDR and 55.8% were resistant to the entire panel of antibiotics tested. All MDR isolates were resistant to levofloxacin and 93% were resistant to meropenem. We identified seven distinct, MDR-specific mutations in nalD, three of which are novel. mexA was significantly overexpressed in strains that were resistant to the entire test antibiotic panel while gyrA and gyrB were overexpressed in MDR isolates. Mutations in fluoroquinolone determinants were significantly associated with MDR phenotype and a novel GyrA Y100C substitution was observed. Carbapenem resistance in MDR isolates was associated with loss-of-function mutations in oprD and high prevalence of NDM (blaNDM-1) within our sample. Conclusions This study provides insight into MDR mechanisms adopted by P. aeruginosa clinical isolates, which may guide the potential development of therapeutic regimens to improve clinical outcomes.

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“…CrpP, an enzyme capable of phosphorylating cipro oxacin, was described in 2018 as encoded in plasmid pUM505 from P. aeruginosa; cloning into J53 increased the MIC to cipro oxacin from 0.008 mg/L to 0.06 mg/L. Since then, crpP-like genes have been reported in E. coli, K. pneumoniae and P. aeruginosa [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of nosocomial Pseudomonas aeruginosa analyzed by pan-genomics

Zhou

Tao

et al. 2023

Preprint

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Pseudomonas aeruginosa is an important hospital-acquired pathogen that is usually resistant to multiple antibiotics. In 2016–2021, among the 103 strains of P. aeruginosa isolated, the age distribution of patients ranged from 10 days to 94 years with a mean age of 66.38 years. The strains isolated were from sputum (72 strains, 69.9%) and blood (14 strains, 13.6%). The size of these genomes ranged from 6.2 Mb to 7.4 Mb, with a mean value of 6.5 Mb. Besides, 20 antibiotics were selected for the determination of the drug sensitivity test. The results of antibiotic sensitivity tests showed that the isolates had the highest resistance rate of 100% to amoxicillin-clavulanate, ampicillin, ampicillin-sulbactam, cefazolin, cefotaxime, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole. In addition to this, a total of 47 crpP genes that mediate resistance to aminoglycoside antibiotics were found distributed on 43 P. aeruginosa strains, and 10 new variants of CrpP were identified, named 1.33, 1.34, 1.35, 1.36, 1.37, 1.38, 1.39, 1.40, 1.41 and 7.1. Whole genome analysis showed that a total of 50 ST types were identified, with ST244 (5/103) and ST1076 (4/103) having a more pronounced distribution advantage. Serotype predictions showed that O6 accounted for 29.13% (30/103), O11 for 23.30% (24/103), O2 for 18.45% (19/103), and O1 for 11.65% (12/103) of the highest proportions. Notably, we found a significantly higher proportion of ExoU in P. aeruginosa strains of serotype O11 than in other Exo-toxins strains. Our study enriches the genomic analysis of other multi-drug resistant P. aeruginosa strains, as well as the study of P. aeruginosa carrying CrpP and its variants.

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Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs

Cited by 7 publications

References 50 publications

Comparative Genomics of Pseudomonas aeruginosa Strains Isolated from Different Ecological Niches

Comparative Genomics of Pseudomonas aeruginosa Strains Isolated from Different Ecological Niches

Comparative molecular profiling of multidrug-resistant Pseudomonas aeruginosa identifies novel mutations in regional clinical isolates from South India

Pathogenicity of nosocomial Pseudomonas aeruginosa analyzed by pan-genomics

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