Resistance to Frost and Tuber Soft Rot in Near-Pentaploid Solanum tuberosum-S. commersonii Hybrids

Carputo, Domenico; Castaldi, Luigi; Caruso, Immacolata; Monti, L. M.; Frusciante, Luigi

doi:10.1270/jsbbs.57.145

Cited by 13 publications

(12 citation statements)

References 21 publications

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“…However, no information is available on the number and structural variability of WRKY TFs in Solanaceae wild species, which represent an important reservoir of genetic variation for breeding. This study was set up with the aim to profile WRKY encoding genes in S. commersonii and S. chacoense, two noteworthy tuber-bearing potato species used in potato breeding programs 20,[43][44][45] .…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that, compared to the cultivated potato 22 , S. commersonii possesses a lower number and S. chacoense a higher number of WRKY genes. Both species displayed a number of WRKYs greater than that of barley (45) 46 , castor bean (58) 47 , cucumber (55) 48 , rapeseed (43) 49 and grapevine (59) 50 , and lower than that of cotton (120) 51 , maize (136) 52 , soybean (131) 53 and rice (100) 25 . From this comparison, it appears that the number of WRKY encoding genes is not proportional to the genome size of the respective plant species, as also reported by Waqas et al 54 .…”

Section: Structural Analysis Of Scwrkys and Schwrkys Revealed Interspmentioning

confidence: 99%

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WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Villano

Esposito

D’Amelia

et al. 2020

Sci Rep

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Wild potatoes, as dynamic resource adapted to various environmental conditions, represent a powerful and informative reservoir of genes useful for breeding efforts. WRKY transcription factors (TFs) are encoded by one of the largest families in plants and are involved in several biological processes such as growth and development, signal transduction, and plant defence against stress. In this study, 79 and 84 genes encoding putative WRKY TFs have been identified in two wild potato relatives, Solanum commersonii and S. chacoense. Phylogenetic analysis of WRKY proteins divided ScWRKYs and SchWRKYs into three Groups and seven subGroups. Structural and phylogenetic comparative analyses suggested an interspecific variability of WRKYs. Analysis of gene expression profiles in different tissues and under various stresses allowed to select ScWRKY045 as a good candidate in woundingresponse, ScWRKY055 as a bacterial infection triggered WRKY and ScWRKY023 as a multiple stressresponsive WRKY gene. Those WRKYs were further studied through interactome analysis allowing the identification of potential co-expression relationships between ScWRKYs/SchWRKYs and genes of various pathways. Overall, this study enabled the discrimination of WRKY genes that could be considered as potential candidates in both breeding programs and functional studies.Plants experience environmental constrains and pathogen attacks during their life. Being sessile organisms, their survival depends on the ability to properly and promptly reprogram cellular networks. Several and different classes of transcription factors (TFs) work as "master regulators" and "selector genes", being able to control processes that specify cell types and developmental patterning and modulate specific pathways. Among them, WRKY factors are drawing a great deal of interest in the scientific community due to their ability to simultaneously cope with multiple stresses 1,2 . They are notorious for coordinating signals in plant immunity response against several pathogens and pest attacks 3,4 . More recently, it has been confirmed that WRKYs also base defence mechanism to abiotic stresses and play a key role in cross-talk pathway networks between plant response and development 5,6 . Their involvement into multiple stress response and in plant growth regulation is evidenced by their W-box specific DNA binding 7,8 . Besides, WRKY binds sugar responsive elements and, very recently, it has been demonstrated that they activate sugar responsive genes through an epigenetic mechanism of control 9 . The systematic classification of components of the WRKY family is well organized. It is based on the WRKY binding domain (WD) characteristics along with those of the Zinc Finger (ZF) motif, which is typically present downstream the WD. WD consists of 60 amino acids structured as four-stranded β-sheets able to enter the major groove of B-form DNA. The highly conserved motif is "WRKYGQK". According to the number of WDs and the type of zinc finger motif, WRKY proteins can be classified into three Groups,...

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Section: Discussionmentioning

confidence: 99%

Section: Structural Analysis Of Scwrkys and Schwrkys Revealed Interspmentioning

confidence: 99%

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Villano

Esposito

D’Amelia

et al. 2020

Sci Rep

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“…It derived from a single seed from accession PI243503 obtained from the Inter-Regional Potato Introduction Station, Sturgeon Bay, Wisconsin (Supplemental Figure 2). This clone has been widely characterized and used in our breeding program as a source of resistance genes to biotic and abiotic stresses (Carputo et al, 1997(Carputo et al, , 2007(Carputo et al, , 2009(Carputo et al, , 2013.…”

Section: Genetic Background Of Sequenced Materialsmentioning

confidence: 99%

The Solanum commersonii Genome Sequence Provides Insights into Adaptation to Stress Conditions and Genome Evolution of Wild Potato Relatives

et al. 2015

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“…These results, combined with those related to glycoalkaloid content, resistance traits and molecular data (Carputo et al , 2007, provide new insight in terms of potato germplasm exploitation and unusual chromosome numbers. We think that this material may give further opportunities to study gamete viability and EBN relationships in plants with unique chromosome numbers.…”

Section: Discussionmentioning

confidence: 86%

“…Some of them have been characterized for other useful traits. MCPH 13 and MCPH 21, for instance, displayed resistance to tuber soft rot, whereas MCPH 22 showed a resistance to low temperatures similar to that of S. commersonii (Carputo et al 2007). These hybrids have been successfully used to generate new progenies with noteworthy morphological and resistance traits (Iovene et al 2004).…”

Section: Discussionmentioning

confidence: 92%

Breeding potential of Solanum tuberosum–S. commersonii pentaploid hybrids: fertility studies and tuber evaluation

Caruso¹,

Castaldi²,

Caruso³

et al. 2008

Euphytica

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Twenty-one (near) pentaploid hybrids between sexually incompatible Solanum commersonii [2x(1EBN)] and cultivated S. tuberosum [4x(4EBN)] were characterized for tuber traits and fertility. A number of genotypes resembled the S. tuberosum type in terms of stolon length and eye depth and produced tubers under long day conditions. Tuber yields were not as high as expected, probably due to lack of adaptation of the S. commersonii genome to the environmental conditions in southern Italy (on average 325 gÁpt -1 and 285 gÁpt -1 in 2003 and in 2004, respectively). Compared to 2004, in the summer of 2003, characterized by extremely high temperatures, hybrids gave a higher tuber yield than the S. tuberosum control, suggesting that in our environmental conditions the wild S. commersonii genome, rather than resulting in heterosis for tuber yield, provides better adaptation to harsh environments. Although aneuploidy has often been associated with reduced fertility, several hybrids were fertile in crosses with S. tuberosum when used as female parents. In particular, the average berry set and number of seeds per berry were 38.2% and 31.8%, respectively. Based on significant relationships between ploidy levels and all yield and fertility data measured, the presence of extra chromosome affected the parameters considered: tuber yield in 2003 and 2004, percentage of fruit set, number of seeds per berry and number of seeds per pollinated flower. Regression analysis also indicated that ''Residuals'' were significant for all parameters measured. Therefore, additional factors (e.g. the genetic makeup of hybrids) may be key to fertility and yield.

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Resistance to Frost and Tuber Soft Rot in Near-Pentaploid Solanum tuberosum-S. commersonii Hybrids

Cited by 13 publications

References 21 publications

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

The Solanum commersonii Genome Sequence Provides Insights into Adaptation to Stress Conditions and Genome Evolution of Wild Potato Relatives

Breeding potential of Solanum tuberosum–S. commersonii pentaploid hybrids: fertility studies and tuber evaluation

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