Resistance to <i>Schistosoma mansoni</i> by transplantation of APO <i>Biomphalaria tenagophila</i>

Barbosa, Luciene; Caldeira, R. L.; Carvalho, Omar S.; Vidigal, Teofânia H. D. A.; Jannotti-Passos, Liana K.; Coelho, Paulo Marcos Zech

doi:10.1111/j.1365-3024.2006.00827.x

Cited by 27 publications

(25 citation statements)

References 19 publications

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“…Several studies have been carried out in order to gain a better understanding of resistance traits, including molecular approaches using nuclear DNA regions (Pires et al 1997, Spatz et al 1999, Barbosa 2001, Rosa et al 2005, Barbosa et al 2006), but little is known about the genes related with the resistance phenotype in this population (Santos et al 1979, de Freitas et al 1985, Rosa 2002.…”

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confidence: 99%

Phylogenetic analysis of Biomphalaria tenagophila (Orbigny, 1835) (Mollusca: Gastropoda)

Jannotti-Passos¹,

Ruiz²,

Caldeira³

et al. 2010

Mem. Inst. Oswaldo Cruz

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mentioning

confidence: 99%

Phylogenetic analysis of Biomphalaria tenagophila (Orbigny, 1835) (Mollusca: Gastropoda)

Jannotti-Passos¹,

Ruiz²,

Caldeira³

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Furthermore, experimental transplantation of several organs from B. glabrata specimens from a strain partially resistant to S. mansoni infection to individuals from a susceptible strain, demonstrated that the APO was unique in its ability of transfering resistance to the receptors (Sullivan & Spence 1994). More recently, Barbosa et al (2006) transformed susceptible strains of B. tenagophila into resistant ones by transplantation of the APO from an absolute resistant strain (Taim, RS, Brazil) of the same mollusk species. The transplanted recipients became completely resistant to S. mansoni infection.…”

mentioning

confidence: 99%

Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

Barbosa

Silva

Coelho³

et al. 2006

Mem. Inst. Oswaldo Cruz

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Biomphalaria glabrata snails are major hosts for the digenetic trematoda Schistosoma mansoni, the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) Schistosomiasis is a parasitic infection caused by the digenetic trematode Schistosoma mansoni Sambom, 1907. The disease is prevalent in 54 countries and territories of Africa, the Caribbean, the Mediterranean, and South America. It is estimated that 130 million people are infected with this species of parasite (Chitsulo et al. 2000). Although several advances have been achieved in the control of the schistosomiasis morbidity, mainly due to the development of new low toxicity and highly effective drugs against the parasite, it is still an endemic disease, whose control and/or eradication is very difficult in the wide regions where it occurs. The affected patients become debilitated, thus impairing the socio-economic development of the affected countries. In Brazil, it is estimated that eight million people are infected, whereas thirty million are at high risk of infection, since they live in hyper endemic areas (Katz 1997 In the S. mansoni-Biomphalaria interaction, besides the stringent physiological and biochemical compatibility between host and parasite, the success or failure of the infection will be strongly determined by the efficacy of the internal defense system of the snail. When in contact with the infective agent, the endogenous defense mechanisms of the mollusk are immediately activated, mediated by circulating effectors cells (hemocytes) and by soluble factors which are present in the hemolymph. Among these factors, lectins with specific carbohydratebinding capacities play an important role by immobilizing foreign objects by agglutination, promoting phagocytes by the hemocytes or even acting as cytophilic receptors for non-self recognition (Van der Knaap & Loker 1990).The hemocytes of Biomphalaria are capable of phagocytosis, encapsulation and, finally, destruction of the infective agents. Both morphologically and functionally, these cells resemble cells of the vertebrate monocytemacrophage series (Jeong et al. 1983, Bezerra et al. 2003. Experimental evidence has been accumulated assigning the production and storage of hemocytes to the hematopoietic organ, a structure that is located on the wall of the pericardium and posterior epithelium of the mollusk mantle. That organ, also known as amebocyte-producing organ or APO, is composed of a small quantity of primary ameboblasts that rest on a loose reticulum, which is formed by extensions of smooth muscles and fibroblasts (Jeong et al. 1983). In a recent study, Sullivan et al. (2004) reported an increase in the frequency of cell mitosis in the APO of B. glabrata following the inoculation of miracidia or cercariae antigens of S. mansoni. The mitotic ratio was intensified in Biomphalaria strai...

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“…Recent studies have confirmed that successful transplantation of the amoebocyte-producing organ from the B. tenagophila Taim isolate to the susceptible B. tenagophila Cabo Frio isolate caused the susceptible organism to be partially refractive to S. mansoni infection (Barbosa et al 2006), indicating that immunological processes play an important role in resistance to infection. In addition, Pereira (2005) and Bezerra and Coelho (2006) showed that inoculation of the hemolymph from B. tenagophila Taim to a susceptible isolate of B. tenagophila promotes a state of resistance against S. mansoni miracidia infection.…”

Section: Discussionmentioning

confidence: 90%

“…Studies with the B. tenagophila Taim lineage have demonstrated its natural resistance to S. mansoni infection and experiments to alter its refractoriness have, until the present, failed (Bezerra et al 2003, Barbosa et al 2006.…”

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confidence: 99%

The effect of early infection with Echinostoma paraensei on the interaction of Schistosoma mansoni with Biomphalaria glabrata and Biomphalaria tenagophila

Garcia¹,

Maldonado²,

Bidau³

et al. 2010

Mem. Inst. Oswaldo Cruz

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Infection caused by the trematode Echinostoma paraensei has been shown to interfere in the natural resistance to infection by Schistosoma mansoni. Biomphalaria glabrata is susceptible to infection, while Taim isolate Biomphalaria tenagophila is resistant to infection by S. mansoni. These two snail species were assessed for infection with E. paraensei two days after exposure to S. mansoni miracidia. The number of B. tenagophila and B. glabrata infected with E. paraensei was lower in co-infected group, suggesting an antagonistic relationship. B. glabrata showed an increase in its susceptibility to S. mansoni, whereas B. tenagophila maintained its refractoriness to S. mansoni infection. Weekly comparisons made between the E. paraensei cercariae released from B. tenagophila and B. glabrata mono-infected snails revealed no quantitative differences. In contrast, S. mansoni cercariae released were higher in the B. glabrata co-infected group. Mortality rates were significantly greater in both species pertaining to co-infected group and unexpected mortalities were also observed in B. tenagophila exposed only to S. mansoni miracidia. Our study revealed that the B. tenagophila Taim isolate is susceptible to E. paraensei infection, although infection did not alter its resistance to S. mansoni infection.

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Resistance to Schistosoma mansoni by transplantation of APO Biomphalaria tenagophila

Cited by 27 publications

References 19 publications

Phylogenetic analysis of Biomphalaria tenagophila (Orbigny, 1835) (Mollusca: Gastropoda)

Phylogenetic analysis of Biomphalaria tenagophila (Orbigny, 1835) (Mollusca: Gastropoda)

Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

The effect of early infection with Echinostoma paraensei on the interaction of Schistosoma mansoni with Biomphalaria glabrata and Biomphalaria tenagophila

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