Resistance to<i>Toxoplasma gondii</i>Infection in Mice Treated with Silk Protein by Enhanced Immune Responses

Moon, Jiwon; Pyo, Kyoung‐Ho; Jung, Byung Mun; Chun, Hyang Sook; Chai, Jong Yil; Shin, Eun Hee

doi:10.3347/kjp.2011.49.3.303

Cited by 10 publications

(8 citation statements)

References 27 publications

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“…In particular, it has been reported that certain protein food products, including lactoferrin, soybean protein fraction (a milk-derived protein casein), contain immune-modulating functions and thus increase NK cell activity in immunocompromised mice, stimulate the cellular immune response, and exert mitogenic effects ( 31 32 33 34 ). Silk protein has also been proposed as a bioactive substance and is effective at reducing blood pressure, exhibiting anti-tumor activity, treating atopic dermatitis, promoting insulin release, and enhancing innate immune responses ( 14 18 35 36 37 ). In this regard, we explored the effects of silk peptide on immune capability in vivo , especially on NK cell activity, in mice.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells

Jang

Oh²,

Baek³

et al. 2018

Immune Netw

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Silk peptide, the hydrolysate of silk protein derived from cocoons, has been employed as a biomedical material and is believed to be safe for human use. Silk peptide display various bioactivities, including anti-inflammatory, immune-regulatory, anti-tumor, anti-viral, and anti-bacterial. Although earlier investigations demonstrated that silk peptide stimulates macrophages and the production of pro-inflammatory cytokines, its effect on natural killer (NK) cell function has not yet been explored. In this study, we initially confirmed that silk peptide enhances NK cell activity in vitro and ex vivo. To assess the modulatory activity of silk peptide on NK cells, mice were fed various amounts of a silk peptide-supplemented diet for 2 months and the effects on immune stimulation, including NK cell activation, were evaluated. Oral administration of silk peptide significantly enhanced the proliferation of mitogen- or IL-2-stimulated splenocytes. In addition, oral silk peptide treatment enhanced the frequency and degree of maturation of NK cells in splenocytes. The same treatment also significantly enhanced the target cell cytolytic activity of NK cells, which was determined by cell surface CD107a expression and intracellular interferon-γ expression. Finally, oral administration of silk peptide stimulated T helper 1-type cytokine expression from splenic lymphocytes. Collectively, our results suggest that silk peptide potentiates NK cell activity in vivo and could be used as a compound for immune-modulating anti-tumor treatment.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells

Jang

Oh²,

Baek³

et al. 2018

Immune Netw

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“…Phenotypic changes in splenocytes were analyzed using flow cytometry [ 44 ]. Spleens were gently crushed through a stainless-steel mesh under aseptic conditions to remove tissue debris.…”

Section: Methodsmentioning

confidence: 99%

Immune adjuvant effect of aToxoplasma gondiiprofilin-like protein in autologous whole-tumor-cell vaccination in mice

et al. 2016

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Profilin-like protein in Toxoplasma gondii (TgPLP) is a Toll-like receptor (TLR) agonist. In this study, we investigated whether TgPLP has an adjuvant effect on immune function in autologous whole-tumor-cell vaccine (AWV) treatment. Mice vaccinated with AWV together with recombinant TgPLP protein had smaller CT26 tumors and increased survival. TgPLP treatment strongly increased the production of IL-12 through MyD88 signaling and several chemokines, including CCL5, CCL12, and XCL1, in bone marrow-derived macrophages (BMMs). In addition, TgPLP increased the phagocytosis of tumor cells by BMMs and promoted immune cell mobility on a tumor-matrigel scaffold. TgPLP triggered immune responses as demonstrated by increased expression of antigen presenting cell markers (MHC class I and II, B7.1, and B7.2) in BMMs and increased IL-12 and IFN-γ expression in mice. Mice vaccinated with AWV and TgPLP had more immune cells (CD4+ and CD8+ T cells, natural killer cells, and macrophages) in the spleen and higher total IgG and IgG2a concentrations in the blood than mice vaccinated with AWV alone. These findings suggest that TgPLP is a TLR-based vaccine adjuvant that enhances antitumor immune responses during vaccination with AWV.

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“…After an acclimation period of 1 week, mice were housed in an animal facility equipped with SPF conditions at the Seoul National University College of Medicine [11]. Seven-week-old C57BL/6 mice were infected with G. seoi metacercariae.…”

mentioning

confidence: 99%

“…Specific pathogen-free (SPF) female C57BL/6 mice were purchased from Koatech Laboratory Animal Center (Pyeongtaek-shi, Gyeonggi-do, Korea). After an acclimation period of 1 week, mice were housed in an animal facility equipped with SPF conditions at the Seoul National University College of Medicine [ 11 ]. Seven-week-old C57BL/6 mice were infected with G. seoi metacercariae.…”

mentioning

confidence: 99%

STAT6 Expression and IL-13 Production in Association with Goblet Cell Hyperplasia and Worm Expulsion of Gymnophalloides seoi from C57BL/6 Mice

et al. 2013

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In intestinal helminth infections, Th2 immune respones are generally associated with mucin secretion for worm expulsion from the host intestine. In particular, IL-4 and IL-13 are the important cytokines related with intestinal mucus production via STAT6 signalling in nematode infections. However, this perspective has never been studied in Gymnophalloides seoi infection. The present study aimed to observe the STAT6 signalling and cytokine responses in C57BL/6 mice, a mouse strain resistant to infection with this trematode. The results showed that worm expulsion occurred actively during days 1-2 post-infection (PI), when goblet cells began to proliferate in the small intestine. The STAT6 gene expression in the mouse spleen became remarkable from day 2 PI. Moreover, G. seoi infection induced a significant increase of IL-13 from day 4 PI in the spleen of infected mice. Our results suggested that goblet cell hyperplasia and worm expulsion in G. seoi-infected mice should be induced by STAT6 signalling, in which IL-13 may be involved as a dominant triggering cytokine.

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Resistance toToxoplasma gondiiInfection in Mice Treated with Silk Protein by Enhanced Immune Responses

Cited by 10 publications

References 27 publications

Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells

Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells

Immune adjuvant effect of aToxoplasma gondiiprofilin-like protein in autologous whole-tumor-cell vaccination in mice

STAT6 Expression and IL-13 Production in Association with Goblet Cell Hyperplasia and Worm Expulsion of Gymnophalloides seoi from C57BL/6 Mice

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