Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish

Tsuduki, Masao; Takano, Toshiyuki; Nakatsubo, Fumiaki; Yoshida, Kazuya; Shinmyo, Atsuhiko; Asao, Hiroshi

doi:10.5511/plantbiotechnology.23.71

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…It was selected because it has been suggested in the literature that lignin content can be related to the favorability of a plant organ as feeding substrate for a pest (Fogan and Gowen 1996). Lignin is one of the main gymnosperm and angiosperm components, mentioned as responsible for the protection of plant tissues against attack by microorganisms (Fengel and Wegener 1989) and arthropods (Tsuduki et al 2006). According to Deschamps (1999), lignin content increases with plant age, what was also found in the present study for the superficial tissues of coconut fruits.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Aceria guerreronis and Neoseiulus baraki among and within coconut bunches in northeast Brazil

et al. 2011

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Aceria guerreronis Keifer (Acari: Eriophyidae) is considered a major pest of coconut in many countries in the Americas, Africa and parts of Asia. Neoseiulus baraki Athias-Henriot (Acari: Phytoseiidae) is one of the predatory mites most commonly found in association with A. guerreronis in parts of northeast Brazil. The objective of this work was to study the distribution of A. guerreronis and N. baraki among and within coconut bunches. The hypothesis was tested that A. guerreronis and N. baraki are homogenously distributed over the fruits in a bunch, independent of the fruits' age and position. Five collections of bunches, each corresponding to leaves 12-16 from apex (about 2-6 month-old), were conducted in each of three fields in northeastern Brazil, from February to October, 2007. A total of 1,986 fruits were examined. The number of mites, the percentage of fruits hosting them and the level of damage caused by A. guerreronis were evaluated. The highest density of A. guerreronis was observed on fruits of bunch 4 whereas the highest density of N. baraki was observed on bunch 5. Considering all fruits together, no significant differences were observed between densities of either A. guerreronis or N. baraki among the basal, median and apical thirds of the bunches. In younger bunches, fruits of the apical region tend to have lower densities of both mites than fruits of the basal region. This pattern, in association with a similar pattern for the percentage of fruits hosting N. baraki, suggests that the predator initially reaches the basal bunch region, from where it moves to the apical region. The results of the present study suggest that the pest population reduction in bunches older than bunch 4 could be due to (1) an effect of the predator, (2) reduction of the proportion of undamaged tissues amenable to attack, and/or (3) less favorable characteristics of the fruits to attack by A. guerreronis, as indicated by their increasing lignin content as they get older.

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Section: Discussionmentioning

confidence: 99%

“…25%, i.e., the lignin concentration in fruits 4 (25%) and 5 (27%). It has been suggested that even small changes in lignin content can have a large impact in plant resistance to insect attack (Tsuduki et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Distribution of Aceria guerreronis and Neoseiulus baraki among and within coconut bunches in northeast Brazil

et al. 2011

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“…A lower than 30% increase in the lignin content was observed in insect‐resistant Solanum plants (Tsuduki et al . ). Moreover, a lignin content of lower than 10% in three maize populations correlated to severe plant damage caused by the European corn borer (Ostrander & Coors ).…”

Section: Discussionmentioning

confidence: 97%

Signal transduction and regulation of IbpreproHypSys in sweet potato

Wan

Lin

et al. 2016

Plant Cell & Environment

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Hydroxyproline-rich glycopeptides (HypSys) are small signalling peptides containing 18-20 amino acids. The expression of IbpreproHypSys, encoding the precursor of IbHypSys, was induced in sweet potato (Ipomoea batatas cv. Tainung 57) through wounding and IbHypSys treatments by using jasmonate and H2 O2 . Transgenic sweet potatoes overexpressing (OE) and silencing [RNA interference (RNAi)] IbpreproHypSys were created. The expression of the wound-inducible gene for ipomoelin (IPO) in the local and systemic leaves of OE plants was stronger than the expression in wild-type (WT) and RNAi plants after wounding. Furthermore, grafting experiments indicated that IPO expression was considerably higher in WT stocks receiving wounding signals from OE than from RNAi scions. However, wounding WT scions highly induced IPO expression in OE stocks. These results indicated that IbpreproHypSys expression contributed towards sending and receiving the systemic signals that induced IPO expression. Analysing the genes involved in the phenylpropanoid pathway demonstrated that lignin biosynthesis was activated after synthetic IbHypSys treatment. IbpreproHypSys expression in sweet potato suppressed Spodoptera litura growth. In conclusion, wounding induced the expression of IbpreproHypSys, whose protein product was processed into IbHypSys. IbHypSys stimulated IbpreproHypSys and IPO expression and enhanced lignin biosynthesis, thus protecting plants from insects.

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“…The bark beetle Dendroctonus micans Hugel feeding on spruce trees with higher bark lignin content had reduced larval weight, survival, and developmental rate (Wainhouse et al 1998). Solanum integrifolium L. plants transformed with horseradish peroxidase gene had 30% higher lignin content compared with wildtype plants and a high level of resistance to corn earworm, Heliothis armigera (Hü bner) (Tsuduki et al 2006). Our previous investigation into the activities of oxidative enzymes such as peroxidase, lipoxygenase, catalase, and polyphenol oxidase in response to the feeding of southern chinch bug on resistant and susceptible St Augustinegrass cultivars revealed that the chinch bug-resistant FX-10 had increased peroxidase activity within 5 d of southern chinch bug infestation (Rangasamy 2008).…”

mentioning

confidence: 99%

“…Because plant peroxidases play diverse roles in plants, including lignin biosynthesis in the cell wall (Lagrimini et al 1987) and oxidation of toxic compounds (Gasper et al 1982) and because ligniÞcation of cells could be an impediment to insect feeding (Coors 1987;Buendgen et al 1990;Wainhouse et al 1990Wainhouse et al , 1998Tsuduki et al 2006), we hypothesized that increased ligniÞcation could be a possible component of chinch bug resistance in diploid Captiva and polyploid FX-10 St. Augustinegrass. In the research reported here, we compared stylet probing frequencies, leaf sheath lignin content, and vascular bundle anatomy among two southern chinch bug-resistant and two susceptible St. Augustinegrass cultivars.…”

mentioning

confidence: 99%

Role of Leaf Sheath Lignification and Anatomy in Resistance Against Southern Chinch Bug (Hemiptera: Blissidae) in St. Augustinegrass

Rangasamy¹,

Rathinasabapathi²,

McAuslane³

et al. 2009

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Southern chinch bug, Blissus insularis Barber (Hemiptera: Blissidae), is the most serious insect pest of St. Augustinegrass Stenotaphrum secundatum (Walter) Kuntze, a common lawngrass grown in southeastern U.S. states. Host plant resistance to southern chinch bug has been identified in the polyploid St. Augustinegrass 'FX-10' and the diploid 'Captiva'. The objective of this research was to identify possible physical mechanism(s) explaining chinch bug resistance in these cultivars. We studied the distribution of chinch bug salivary sheaths in the preferred tissue for feeding (the axillary shoot) of the two resistant cultivars and two susceptible cultivars, paired for ploidy ('Floratam', polyploid, and Palmetto, diploid). We also investigated the potential role of axillary shoot lignification and anatomy in chinch bug resistance. Salivary sheaths were more abundant on the outermost leaf sheath of axillary shoots of resistant cultivars compared with susceptible cultivars. In contrast, fewer salivary sheaths reached the innermost meristematic tissue in the axillary shoots of resistant St. Augustinegrass cultivars than in the two susceptible cultivars. The polyploid cultivars FX-10 and Floratam had higher total lignin in axillary shoots compared with the diploid cultivars Captiva and Palmetto. However, total lignin content was not correlated with resistance to southern chinch bug. Light microscopic studies found no differences in epidermal layer thickness among resistant and susceptible St. Augustinegrass cultivars. However, transmission electron microscopic studies revealed that the cell walls of the sclerenchyma cells around the vascular bundle of southern chinch bug-resistant FX-10 and Captiva were significantly thicker than the cell walls in susceptible Floratam and Palmetto. Our research suggests that the thick-walled sclerenchyma cells around the vascular bundle play a role in southern chinch bug resistance in St. Augustinegrass, possibly by reducing stylet penetration to the vascular tissue.

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Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish

Cited by 6 publications

References 15 publications

Distribution of Aceria guerreronis and Neoseiulus baraki among and within coconut bunches in northeast Brazil

Distribution of Aceria guerreronis and Neoseiulus baraki among and within coconut bunches in northeast Brazil

Signal transduction and regulation of IbpreproHypSys in sweet potato

Role of Leaf Sheath Lignification and Anatomy in Resistance Against Southern Chinch Bug (Hemiptera: Blissidae) in St. Augustinegrass

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