Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8

Næss, Sigrid; Bradeen, James M.; Wielgus, Susan M.; Haberlach, Geraldine T.; McGrath, J. Mitchell; Helgeson, John P.

doi:10.1007/s001220051533

Cited by 144 publications

(77 citation statements)

References 31 publications

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“…Transgenic Katahdin plants with the RB gene developed limited lesions on the lower leaves consistent with symptoms noted in field evaluations of the RB-containing progenies derived from S. bulbocastanum clone PT29 (5). The RB locus on chromosome 8 explains 62% of the genetic variation in late blight resistance in the progenies derived from S. bulbocastanum (8). Quantitative trait loci (QTL)-associated late blight resistances, which have been identified in various potato populations (33)(34)(35), may also contribute to the resistance of S. bulbocastanum clone PT29.…”

Section: Discussionmentioning

confidence: 99%

“…A major resistance locus, RB, was mapped to a specific location on chromosome 8 of S. bulbocastanum (8). A bacterial artificial chromosome (BAC) contig spanning the RB gene was constructed by using a reiterative approach of BAC walking and high-resolution genetic mapping (9).…”

Section: P Otato Late Blight a Disease Caused By The Oomycete Pathogenmentioning

confidence: 99%

“…The RB locus was previously mapped to chromosome 8 of S. bulbocastanum (8). The RB locus is heterozygous (RB͞rb) in the original S. bulbocastanum clone PT29 used in the development of genetic mapping populations and BAC libraries.…”

Section: Cloning Of the Rb Gene By Lr-pcrmentioning

confidence: 99%

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Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight

Song

Bradeen

Næss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

543

352

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Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating potato disease in the world. Control of late blight in the United States and other developed countries relies extensively on fungicide application. We previously demonstrated that the wild diploid potato species Solanum bulbocastanum is highly resistant to all known races of P. infestans. Potato germplasm derived from S. bulbocastanum has shown durable and effective resistance in the field. Here we report the cloning of the major resistance gene RB in S. bulbocastanum by using a map-based approach in combination with a long-range (LR)-PCR strategy. A cluster of four resistance genes of the CC-NBS-LRR (coiled coil-nucleotide binding site-Leu-rich repeat) class was found within the genetically mapped RB region. Transgenic plants containing a LR-PCR product of one of these four genes displayed broad spectrum late blight resistance. The cloned RB gene provides a new resource for developing late blight-resistant potato varieties. Our results also demonstrate that LR-PCR is a valuable approach to isolate genes that cannot be maintained in the bacterial artificial chromosome system.

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Section: Discussionmentioning

confidence: 99%

Section: P Otato Late Blight a Disease Caused By The Oomycete Pathogenmentioning

confidence: 99%

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Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight

Song

Bradeen

Næss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

543

352

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“…Examples are S. pinnatisectum (Kuhl et al 2001) and S. bulbocastanum (Naess et al 2000;Song et al 2003; Van der Vossen et al 2003 andPark et al 2005) from Mexico and S. berthaultii (Rauscher et al 2006;Ewing et al 2000), S. microdontum (Sandbrink et al 2000), S. mochiquense (Smilde et al 2005) and S. paucissectum (Villamon et al 2005) from Andean countries. In addition, the cultivated diploid S. phureja has been described to contain valuable resistance (Ghislain et al 2001;Sliwka et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

et al. 2008

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Allele mining facilitates the discovery of novel resistance (R) genes that can be used in breeding programs and sheds light on the evolution of R genes. Here we focus on two R genes, Rpi-blb1 and Rpi-blb2, originally derived from Solanum bulbocastanum. The Rpi-blb1 gene is part of a cluster of four paralogues and is Xanked by RGA1-blb and RGA3-blb. Highly conserved RGA1-blb homologues were discovered in all the tested tuber-bearing (TB) and non-tuber-bearing (NTB) Solanum species, suggesting RGA1-blb was present before the divergence of TB and NTB Solanum species. The frequency of the RGA3-blb gene was much lower. Interestingly, highly conserved Rpi-blb1 homologues were discovered not only in S. bulbocastanum but also in Solanum stoloniferum that is part of the series Longipedicellata. Resistance assays and genetic analyses in several F1 populations derived from the relevant late blight resistant parental genotypes harbouring the conserved Rpi-blb1 homologues, indicated the presence of four dominant R genes, designated as Rpi-sto1, Rpi-plt1, Rpi-pta1 and Rpi-pta2. Furthermore, Rpi-sto1 and Rpi-plt1 resided at the same position on chromosome VIII as Rpi-blb1 in S. bulbocastanum. Segregation data also indicated that an additional unknown late blight resistance gene was present in three populations. In contrast to Rpi-blb1, no homologues of Rpi-blb2 were detected in any material examined. Hypotheses are proposed to explain the presence of conserved Rpi-blb1 homologues in S. stoloniferum. The discovery of conserved homologues of Rpi-blb1 in EBN 2 tetraploid species oVers the possibility to more easily transfer the late blight resistance genes to potato varieties by classical breeding.

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“…Recently two resistance genes Rpi-blb1/ RB and Rpi-blb2 derived from S. bulbocastanum were cloned (Song et al 2003;van der Vossen et al 2003van der Vossen et al , 2004. The RB gene was localized on chromosome 8 (Naess et al 2000). Because of the inability of direct sexual crosses between S. tuberosum and S. bulbocastanum, BC 2 populations derived from somatic hybrids were used (Helgeson et al 1998).…”

Section: Introductionmentioning

confidence: 99%

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

et al. 2005

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Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.

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Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8

Cited by 144 publications

References 31 publications

Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight

Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

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