Resistance to quinolones, cephalosporins and macrolides in Escherichia coli causing bacteraemia in Peruvian children

Palma, Noemí; Pons, María J.; Gomes, Cláudia; Mateu, Javier; Riveros, Maribel; García, Wilfredo; Jacobs, Jan; García, Coralith; Ochoa, Theresa J.; Ruiz, Joaquı́m

doi:10.1016/j.jgar.2017.06.011

Cited by 46 publications

(58 citation statements)

References 17 publications

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“…Thus, the MIC of LVX was established by the agar dilution method 39 both with (MIC PAβN ) 20 µg/ml and without (MIC I ) of PAβN. An EPO phenotype was defined when MIC I /MIC PABN was >2 as previously described 40 . The effect of this concentration of PAβN on the viability of microorganisms was also assessed.…”

Section: Methodsmentioning

confidence: 99%

Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

Horna

López

Guerra

et al. 2018

Sci Rep

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MexAB-OprM and MexEF-OprN are Pseudomonas aeruginosa efflux pumps involved in the development of antibiotic resistance. Several studies developed with laboratory strains or using a few clinical isolates have reported that the regulation system of MexEF-OprN is involved in the final levels of MexAB-OprM expression. Therefore, this study was aimed to determine the interplay between MexAB-OprM and MexEF-OprN in 90 out of 190 P. aeruginosa clinical isolates with an efflux pump overexpression phenotype. Regarding oprD, 33% (30/90) of isolates displayed relevant modifications (RM) defined as frameshift or premature stop, both related to carbapenem resistance. On the other hand, 33% of the isolates displayed RM in nalC, nalD or mexR, which were significantly associated with multidrug resistance (MDR), non-susceptibility to carbapenems, OprD alterations and strong biofilm production. Meanwhile, the RM in MexS were associated with presence of pigment (p = 0.004). Otherwise, when all the regulators were analysed together, the association between RM in MexAB-OprM regulators and MDR was only significant (p = 0.039) when mexS was the wild type. These data show the modulatory effect of MexEF-OprN on MexAB-OprM in a clinical population of P. aeruginosa. Further studies may contribute to design of novel molecules acting on this interplay to fight against antimicrobial resistance.

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Section: Methodsmentioning

confidence: 99%

Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

Horna

López

Guerra

et al. 2018

Sci Rep

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“…En la mayoría de los países de bajos y medianos ingresos el problema está subestimado, principalmente por la escasez de estudios en estas regiones. Asimismo, los datos disponibles actualmente han mostrado un escenario preocupante, con altos niveles de resistencia a los antimicrobianos usados comúnmente (7) . La presencia de betalactamasas de espectro extendido (BLEE) es uno de los mecanismos de resistencia a antimicrobianos más importantes (7) .…”

Section: Mensajes Clave Introducciónunclassified

“…Asimismo, los datos disponibles actualmente han mostrado un escenario preocupante, con altos niveles de resistencia a los antimicrobianos usados comúnmente (7) . La presencia de betalactamasas de espectro extendido (BLEE) es uno de los mecanismos de resistencia a antimicrobianos más importantes (7) . Estas enzimas suelen estar codificadas en las mismas estructuras genéticas que otros mecanismos de resistencia, lo que da lugar a fenotipos de multirresistencia a los antibióticos.…”

Section: Mensajes Clave Introducciónunclassified

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Presencia de Enterobacteriaceae y Escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en Lima

Ruiz-Roldán

Martínez-Puchol

Gomes

et al. 2018

Rev Peru Med Exp Salud Publica

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Objetivos. El objetivo del presente estudio fue describir la presencia de Enterobacteriaceae en muestras de carne recolectadas en mercados tradicionales de Lima y establecer los niveles de resistencia a antimicrobianos y la presencia de betalactamasas de espectro extendido (BLEE) y AmpC en Escherichia coli. Materiales y métodos. Se recolectaron un total de 138 muestras de carne, 64 (46,4 %) de pollo, 44 (31,9 %) de carne de res y 30 (21,7%) de carne de cerdo. Las bacterias aisladas pertenecieron a 17 géneros diferentes, y específicamente 14 fueron clasificados como Enterobacteriaceae. Se analizó la sensibilidad frente a diez agentes antimicrobianos mediante el método de difusión de disco Kirby-Bauer, se determinó la presencia de BLEE y AmpC mediante las pruebas de doble disco y de inducción de imipenem-ceftazidima, respectivamente. Resultados. Los niveles de resistencia a los antimicrobianos fueron altos frente a trimetoprima-sulfametoxazol, ampicilina, tetraciclina, ácido nalidíxico, ciprofloxacino y cloranfenicol. Existen diferencias significativas en los niveles de resistencia a los antibióticos según el tipo de carne (pollo, carne de res y cerdo) (p <0,05). Los niveles de resistencia a múltiples antimicrobianos (MDR) fueron particularmente altos en pollo y cerdo (98,2 % y 86,4 %, respectivamente). Además, la presencia de BLEE en Escherichia coli aisladas de carne de pollo fue del 59,4 %. Conclusiones. Los niveles de resistencia a los antimicrobianos fueron altos frente a los antibióticos usados frecuentemente en humanos, se destaca el pollo y la res como potenciales reservorios de Escherichia coli productoras de BLEE y pAmpC, respectivamente.

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“…CTX-M group 9 (bla CTX-M 65 ) was found in 24 of our S. infantis isolates with resistance to CTX and CRO and 4 out of 24 (17%) with decreased susceptibility to CAZ, characteristic of an isolate carrying the bla CTX-M gene. 36 Although bla CTX-M 65 has been frequently found in neighboring countries 37 and has been observed in strains of Escherichia coli causing bacteremia in children 22 in Peru, to our knowledge, this is the first report in Peru of the bla CTX-M 65 gene in Salmonella strains.…”

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confidence: 84%

Presence of Extended-Spectrum β-lactamase, CTX-M-65 in Salmonella enterica serovar Infantis Isolated from Children with Diarrhea in Lima, Peru

Granda

Riveros

Martínez-Puchol

et al. 2019

J Pediatr Infect Dis

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Gastroenteritis in children is a serious condition in many parts of the world. Salmonella enterica is one of the causes of the disease. In this study, 280 fecal samples from children with diarrhea in four hospitals in Lima, Peru, were collected between September 2012 and March 2013. Salmonella was detected in 26 of the samples. Serotyping demonstrated that 25 of the isolates were S. enterica Infantis, and one isolate was S. enterica Typhimurium. Repetitive extragenic palindromic–polymerase chain reaction analysis suggests that all S. Infantis belong to the same clone. All but one of the S. Infantis isolates exhibited an extended-spectrum β-lactamase phenotype as they harbored bla CTX-M 65. Two strains also carried bla TEM-1. Nine of the isolates were resistant to azithromycin and two to ciprofloxacin. This study demonstrates that a multidrug-resistant S. Infantis clone carrying bla CTX-M 65 was circulating among children in Lima, Peru. The development of molecular epidemiology studies in Salmonella-causing diarrhea or other pathologies in Lima and in other areas will be useful to determine the permanence, geographical spread, and clinical implications of this clone.

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Resistance to quinolones, cephalosporins and macrolides in Escherichia coli causing bacteraemia in Peruvian children

Abstract: A variety of ESBL-encoding genes and widespread of bla in Lima has been shown. The role of efflux pumps in azithromycin resistance needs to be further evaluated, as well as effective control of the use of antimicrobial agents.

Cited by 46 publications

References 17 publications

Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

Presencia de Enterobacteriaceae y Escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en Lima

Presence of Extended-Spectrum β-lactamase, CTX-M-65 in Salmonella enterica serovar Infantis Isolated from Children with Diarrhea in Lima, Peru

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