“…In the case of tylosin, the methylation at position A2058 does not by itself result in the development of resistance, but rather the concomitant methylation of other 23S rRNA positions, such as G745 or G748 in Gram-negative and Gram-positive microorganisms, respectively is needed (Liu & Douthwaite, 2002a, b). Thus, among others, Erm(32) (formerly TlrB or RlmA II ) encoded in the chromosome of members of the Streptomyces genus has been described to be able to methylate position G748 (Liu & Douthwaite, 2002a). In E. coli the presence of a related methylase, called RlmA I (formerly RrmA) has been described which acts by methylating position G745 (Liu & Douthwaite, 2002b).…”