Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

Abstract

RESUMO.-[Resistência a β-lactâmicos e tetraciclina emCampylobacter spp. isolados de matadouros-frigorífi-cos de aves no sul do Brasil.] O presente estudo foi realizado para examinar e analisar as características genéticas de resistência antimicrobiana de Campylobacter spp. a partir de fontes avícolas. Um total de 141 amostras de Campylobacter isolados em matadouros-frigoríficos de aves do estado do Rio Grande do Sul, Brasil, foi identificado por mé-todos fenotípicos e genotípicos. Foi analisada a susceptibilidade antimicrobiana e a presença de genes de resistência.As cepas foram testadas para detectar sensibilidade frente a dois antimicrobianos (ampicilina e tetraciclina) pelo método de difusão em disco. A seguir, usando a reação em cadeia da polimerase foi confirmada a espécie e a presença dos genes de resistência à ampicilina (bla OXA-61 ) e tetraciclina tet(O), assim como a detecção da bomba de efluxo (cmeB). Campylobacter jejuni foi a espécie mais isolada, sua presença foi determinada em 140 amostras (99,3%), e Campylobacter coli foi encontrada em uma única amostra (0,70%). Os resultados obtidos mostraram 65% e 35,5% de Campylobacter isolados resistentes a β-lactâmicos e tetraciclinas, respectivamente. O gene cmeB responsável pela resistência a múltiplos antimicrobianos foi detectado em 26 amostras (18,5%). Neste contexto, 36 das 141 amostras (25,6%) foram consideradas resistentes a dois grupos diferentes de antimicrobianos (β-lactâmicos e tetraciclinas). Campylobacter isolated from samples of broilers of slaughterhouses in southern Brazil was identified by phenotypic and genotypic methods. Campylobacter isolates were evaluated for its antimicrobial susceptibility and the presence of resistance genes. The strains were investigated for antimicrobial susceptibility against two agents (ampicillin and tetracycline) by disk diffusion method. PCR assay was used to confirm the specie and the presence of ampicillin (bla OXA-61 ), tetracycline tet(O), and the energy-dependent multi-drug efflux pump (cmeB) genes. Campylobacter jejuni was the most ubiquitous; its presence was determined in 140 samples out of 141 (99.3%), whereas Campylobacter coli was found only in one of the contaminated samples (0.70%). The results obtained showed 65% and 35.5% of Campylobacter isolates resistant to β-lactams and tetracyclines, respectively. The cmeB gene responsible for multidrug resistance was detected in 26 isolates out 141 strains (18.5%). Moreover, 36 out of 141 Campylobacter strains (25.6%) were found to be resistant to at least two different antimicrobia resistance markers (β-lactams and tetracyclines). INDEX TERMS:Campylobacter, tetracycline, β-lactam, efflux pump, resistance genes, PCR. Yuli M. Sierra-Arguello et al.

Antimicrobial Resistance in Campylobacter jejuni Isolated from Brazilian Poultry Slaughterhouses

“…Emerging resistance to the antimicrobial agents of choice for treating Campylobacter infections is becoming a serious threat to public health (Du et al, 2018). The frequency of MDR strains found in this study is similar to that in previous reports from Brazil (Sierra-Arguello et al, 2015). Given such results, Brazilian authorities should consider establishing an integrated surveillance network for antibiotic resistance in Campylobacter.…”

“…A total of 28 isolates had tetracycline MICs ≥2 mg/L, and 42.8% of them carried the gene. Reports from Brazil have shown lower frequencies of this gene than in other countries (Sierra-Arguello et al, 2015;Du et al, 2018). This gene can be present in conjugative plasmids containing resistance genes for other antimicrobials that continue to undergo selective pressure.…”

“…We also observed a high level of resistance to tetracycline. Tetracycline resistance in Campylobacter has been previously reported in strains isolated from animal products (Abdi-Hachesoo et al, 2014;Giacomelli et al, 2014;Hungaro et al, 2015;Sierra-Arguello et al, 2015). Over the past decade, the tetracycline compound class has been used in farm animal production as a growth promoter and for the treatment of diseases.…”

Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland

“…Ampicillin, like tetracycline, shows activity against Campylobacter, but in general, both are not recommended for the treatment of campylobacteriosis because the rates of resistance of these antimicrobials are too high to be useful [62]. The results obtained by these authors showed that 65% of Campylobacter isolates from broiler slaughterhouses in southern Brazil were resistant to β-lactams.…”

Diferentes Técnicas De Extração Da Própolis Vermelha De Alagoas: Rendimento E Análise De Compostos Fenólicos

“…É um mecanismo adquirido por mutação genética relacionada com o gene cmeB que altera a expressão das proteínas reguladoras das bombas de efluxo natural da bactéria, processo que induz o transporte ativo do antibiótico de dentro para fora da membrana, impedindo sua ação. Apesar de ser um mecanismo comum celular, com função de excreção de substratos naturais do metabolismo, a bactéria passa a utilizar este mecanismo através da codificação do gene cmeB que, com o gasto energético, faz com que as bombas expulsem o fármaco que ultrapassou a parede celular (ALCALDE-RICO et al, 2016;SIERRA-ARGUELLO et al, 2015).…”