Resistances in Rice to Tungro-Associated Viruses

Hibino, Hiroki; Daquioag, R. D.; Mesina, E. M.; Aguiero, V. M.

doi:10.1094/pd-74-0923

Cited by 34 publications

(17 citation statements)

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“…Thousands of rice accessions have been screened for RTSV resistance at IRRI (Hibino et al, 1990). Many of these accessions were very susceptible to RTSV, but none of them was sensitive enough to show distinct symptoms of infection.…”

Section: Discussionmentioning

confidence: 99%

“…RTSV is transmitted independently, whereas RTBV depends on RTSV for its transmission by vector (Hibino et at., 1979;Hibino, 1983). Those that have access to plants infected with both viruses transmit either one or both viruses in varying frequencies depending on the rice variety tested (Hibino et al, 1990). RTBV alone causes yellowing and stunting symptoms which are enhanced when RTSV is also present.…”

Section: Introductionmentioning

confidence: 99%

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Oryza glaberrima:An indicator plant for rice tungro spherical virus

Cabauatan¹,

Kobayashi²,

Ikeda³

et al. 1993

International Journal of Pest Management

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The accession of Oryza glaberrima (IRGC Acc. no. 100139) was identified and evaluated as an indicator plant for rice tungro spherical virus (RTSV), a latent virus of rice. This accession was susceptible and sensitive to RTSV infection. When 7-day-old seedlings were inoculated with viruliferous leafhopper Nephotettix virescens, the infected plants showed stunting, reduced tillering, and pale green leaves 3-4 weeks after inoculation. The visual evaluation of infected plants corresponded well with the serological assay. Accession 100139 could also be used to distinguish plants infected either with rice tungro bacilliform virus (RTBV) or both RTBV and RTSV, based on characteristic symptoms and the life span of infected plants. The indicator plant would be useful in studying the epidemiology of rice tungro viruses without the need for expensive serological assays.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oryza glaberrima:An indicator plant for rice tungro spherical virus

Cabauatan¹,

Kobayashi²,

Ikeda³

et al. 1993

International Journal of Pest Management

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“…Extensive breeding programmes, conducted at International Rice Research Institute (IRRI), based on the screening of rice germplasm collections led to the identification of a number of rice cultivars resistant to RTD (Hibino et al, 1990;Khush et al, 2004;Sebastian et al, 1996). Several studies then focused on the Indica rice cultivar Utri Merah, which is resistant to both RTSV and RTBV (Azzam et al, 2001;Cabunagan et al, 1999;Ebron et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Macovei

Sevilla

Cantos

et al. 2018

Plant Biotechnology Journal

335

146

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SummaryRice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of RTD in the field. Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). The Y1059V1060V1061 residues of eIF4G are known to be associated with the reactions to RTSV. To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV‐susceptible variety IR64, widely grown across tropical Asia. The mutation rates ranged from 36.0% to 86.6%, depending on the target site, and the mutations were successfully transmitted to the next generations. Among various mutated eIF4G alleles examined, only those resulting in in‐frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance. Furthermore, our data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eIF4G could not be maintained in homozygous state. The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties.

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“…However, the designation of most of these strains was made before it was recognized that tungro was a complex of two viruses. Studies which indicate that rice cultivars react differentially to RTBV and/or RTSV, and express variable symptoms depending on single or double infection by these viruses (Hibino et al, 1987(Hibino et al, , 1990Dahal et al, 1990b) also confuse the recognition of strains. Recently, two variants (strains) of RTSV have been recognized in the Philippines using their reaction in rice cultivar TKM6 and four strains of RTBV have been recognized from their symptoms in cultivar FK135 Cabauatan et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Variation in the genome of rice tungro bacilliform virus: molecular characterization of six isolates

Fan

Dahal

Dasgupta

et al. 1996

Journal of General Virology

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The DNA genomes of isolates office tungro bacilliform virus from Bangladesh, India, Indonesia, Malaysia and Thailand were cloned and compared with that of the type isolate from the Philippines. Restriction endonuclease maps revealed differences between the isolates and cross-hybridization showed that they fell into two groups, those from the Indian subcontinent and those from south-east Asian countries. The genomes of isolates from the Indian subcontinent contained a deletion of 64 bp when compared with those from south-east Asia. The implications of this variation are discussed.

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Resistances in Rice to Tungro-Associated Viruses

Cited by 34 publications

References 0 publications

Oryza glaberrima:An indicator plant for rice tungro spherical virus

Oryza glaberrima:An indicator plant for rice tungro spherical virus

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

Variation in the genome of rice tungro bacilliform virus: molecular characterization of six isolates

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