Resistant enterococci isolated from raw sheep’s milk and cheeses from South region of Brazil

Souza, Débora Buzatto de; Pereira, Rebeca Inhoque; Endres, Creciana Maria; Frazzon, Jeverson; Prichula, Janira; Frazzon, Ana Paula Guedes

doi:10.1590/0103-8478cr20220288

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…The second is the Rural Insurance Premium Subsidy Program (PSR), a government initiative that provides monetary resources to (re)insurance companies, allowing them to reduce risk premiums for farmers. As reported by Souza et al (2022), from 2013 to 2021, the PSR program experienced a substantial growth of 108%, achieving an insured capital of R$ 67 billion by 2021. However, the PROAGRO program has lost its place with a decrease of 39% in the same period and was able to cover a capital of R$ 17 billion in 2021.…”

Section: Severitymentioning

confidence: 96%

“…Layer 1 Layer 2 Layer 3 In the context of Brazil, there are two major programs that offer risk mitigation for agriculture (Souza et al, 2022). The first is the Agricultural Activity Guarantee Program (Programa de Garantia da Atividade Agropecuária -PROAGRO), which is designed for small and medium farmers and aims at safeguarding income for farmers in case of extreme events.…”

Section: Severitymentioning

confidence: 99%

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Development of index insurance under the dynamics of multi-hazard risk in the context of climate change

Benso

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Section: Severitymentioning

confidence: 96%

Section: Severitymentioning

confidence: 99%

Development of index insurance under the dynamics of multi-hazard risk in the context of climate change

Benso

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Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance

Hanzelová,

Dudriková,

Lovayová

et al. 2024

Life

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Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are E. faecalis and E. faecium, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the Enterococcus genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus Enterococci (E. faecalis, E. faecium, E. durans, E. devriesi, E. hirae, E. italicus, E. casseliflavus, E. malodoratus, and E. gallinarum) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of vanA (4.41%) and tetM (14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of “One Health”.

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Genomic evaluation of the probiotic and pathogenic features of Enterococcus faecalis from human breast milk and comparison with the isolates from animal milk and clinical specimens

Badr,

Yasir,

Alkhaldy

et al. 2024

PeerJ

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Enterococcus faecalis is considered a probiotic, commensal lactic acid bacterium in human breast milk (HBM), but there are circulating antibiotic resistant and virulence determinants that could pose a risk in some strains. The study aimed to conduct genomic analysis of E. faecalis isolates recovered from HBM and animal milk and to evaluate their probiotic and pathogenic features through comparative genomics with isolates from clinical specimens (e.g., urine, wound, and blood). Genomic analysis of 61 isolates was performed, including E. faecalis isolates recovered from HBM in Saudi Arabia. Genome sequencing was conducted using the MiSeq system. The fewest antibiotic resistance genes (lsaA, tetM, ermB) were identified in isolates from HBM and animal milk compared to clinical isolates. Several known and unknown mutations in the gyrA and parC genes were observed in clinical isolates. However, 11 virulence genes were commonly found in more than 95% of isolates, and 13 virulence genes were consistently present in the HBM isolates. Phylogenetically, the HBM isolates from China clustered with the probiotic reference strain Symbioflor 1, but all isolates from HBM and animal milk clustered separately from the clinical reference strain V583. Subsystem functions 188 of 263 were common in all analyzed genome assemblies. Regardless of the source of isolation, genes associated with carbohydrate metabolism, fatty acid, and vitamin biosynthesis were commonly found in E. faecalis isolates. In conclusion, comparative genomic analysis can help distinguish the probiotic or pathogenic potential of E. faecalis based on genomic features.

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Resistant enterococci isolated from raw sheep’s milk and cheeses from South region of Brazil

Cited by 3 publications

References 32 publications

Development of index insurance under the dynamics of multi-hazard risk in the context of climate change

Development of index insurance under the dynamics of multi-hazard risk in the context of climate change

Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance

Genomic evaluation of the probiotic and pathogenic features of Enterococcus faecalis from human breast milk and comparison with the isolates from animal milk and clinical specimens

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