Resistencia a antibióticos de bacterias aisladas de biopelículas en una planta de alimentos

Vanegas, Laguna; Correa, Constanza; Morales, Marinees; Martinez, Luis A.; Rugeles, González; Jiménez, I Francy

doi:10.21897/rmvz.350

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…Such a distribution had already been observed in a smaller group of strains [29]. Other studies with different Bacillus species have also shown variable capacities to form biofilms in vitro [6,25,32]. It is important to point out that more than 90% of the studied Bt strains were able to form biofilms.…”

Section: Adhesion Capacity Of Bacillus Thuringiensis Spores and Its Rsupporting

confidence: 55%

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

León¹

2015

SOJMID

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Open Access Research Articlehaving an attenuated immuneresponse [7].It is known that Bt spores and those of other Bacillus species, presenting exosporium, have a higher hydrophobicity, conferring them a higher adhesive potential to diverse materials [8,9], such as those used in industrial food processing [1,[10][11][12][13]. To this respect, there are several reports on the in vitro capacity of these bacteria to adhere to stainless steel laminae and/or synthetic polymers [14][15][16][17][18].Bacterial adhesion to a surface is a two-phases process: in the first phase, planktonic bacteria are moved to a surface by the effect of physical forces such as Brownian motion, van der Waals attraction forces, gravitational forces, the effect of surface electrostatic charge and hydrophobic interactions; in the second phase of adhesion, molecular irreversible reactions between bacterial surface structures and substratum surfaces become predominant [19,20]. Adhesion is an essential stage for the formation and subsequent growth of biofilms [19,21,22].Biofilms are defined as microbial communities embedded in a polymer layer or matrix adhered to an inert surface or substrate, or to living cells or tissues [23,24]. Several members of the Bacillus genus are capable of forming biofilms in vitro at variable magnitudes dependent on the incubation time [6,25]. According to Auger et al. [25], less than 50% of the B. cereus and Bt strains showed capacity to form biofilm in their assays. Formation of biofilms has been related also to the resistance of these species to diverse antibiotics and disinfectants [26,27] found that the latent spores of B. cereus were more efficient in starting and forming biofilms as compared to bacterial cells in vegetative or sessile state. These same authors evidenced that the production of biofilm in cultures with spores depends on the growth curves, which does not occur in cultures in suspension, and could be related to the resistance of the biofilm to adverse factors.Despite the existence of important collections of Bt isolated from different regions of Mexico, few studies have focused on the possible associations and distribution of features that could contribute to the analysis and a better understanding of the relations at the level of species and subspecies of the very AbstractSpores of Bacillus thuringiensis (Bt), used as bioinsecticide, represent a potential contamination risk of the equipment used for food processing and could also induce potential gastrointestinal symptoms in special cases of susceptible individuals. The objective of this work was to explore the in vitro adhesion capacity of Bt spores and its relation to the ability to form biofilms. The adhering capacity of spores of 65 Bt strains was determined in stainless steel tubes, as well as their ability to form biofilms on polystyrene microplates. We analyzed the relation of these two features. All strains showed a variable adhering capacity to stainless steel, ranging from 0.04% to 1.21% of the initial spore load of ~1.7×10 7 CFU/ mL. Th...

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Section: Adhesion Capacity Of Bacillus Thuringiensis Spores and Its Rsupporting

confidence: 55%

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

León¹

2015

SOJMID

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“…65% of isolates had an index ≥ 0.2, indicating that the bacterium has multiple resistance (Vanegas et al, 2009). These outcomes have a huge impact when selecting empiric treatment of these infections.…”

Section: Resultsmentioning

confidence: 99%

Anti-biofilm activity of ibuprofen and diclofenac against some biofilm producing Escherichia coli and Klebsiella pneumoniae uropathogens

Baldiris¹,

TeherÃ¡n²,

Vivas‐Reyes³

et al. 2016

Afr. J. Microbiol. Res.

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Ibuprofen (IBU) and Diclofenac (DIC) are non-steroidal anti-inflammatory drug which have shown to have broad antimicrobial activity and limit biofilm formation. Aim of this study was to evaluate the effects of IBU and DIC against biofilm formation strong strains Escherichia coli and Klebsiella pneumoniae associated with urinary tract infections (UTIs). Additionally some virulence factors, antibiotic resistance, ESBL production were estimated to find correlation with the biofilm production and acil homoserine lactone (AHL). Our results suggest that IBU and DIC, could be useful in the treatment of urinary tract infections caused by E. coli and K. pneumoniae. IBU with CMIB 8, 30 and 125 mg/L, caused dramatic reductions in some cases and inhibited virulence factors which changes in the morphotypes expressed on congo red agar. DIC with CMIB 30 and 50 mg/L caused reductions in biofilm formation 50.1%. In this study, were evidenced the relationship between virulence factors, production of AHL and multi drugs resistance with biofilm production.

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“…(31) About a third of the methicillin-resistant isolates were also resistant to other antibiotics including macrolides, lincosamides, quinolones, aminoglycosides, rifamycins, oxazolidinones, nitrofurans and antifolates. In addition, if a MAR index value >0.2 can be considered a multiple antimicrobial resistance characteristic (58), the presence of these multidrug-resistant strains could support the emergence of the three hypothetical scenarios described above.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance trends in methicillin-resistant and methicillin-susceptible Staphylococcus aureus and Staphylococcus epidermidisisolates obtained from patients admitted to intensive care units. 2010-2015

et al. 2019

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Introducción. La aparición y la diseminación de cepas resistentes en hospitales, principalmente en unidades de cuidado intensivo (UCI), se han convertido en un serio problema de salud pública.Objetivo. Analizar la tendencia de los fenotipos de resistencia de Staphylococcus aureus y Staphylococcus epidermidis resistentes y susceptibles a meticilina aislados en pacientes atendidos en UCI de un hospital de alta complejidad de Cartagena, Colombia, del 2010 al 2015.Materiales y métodos. Estudio analítico transversal realizado entre enero de 2010 y diciembre de 2015. Se utilizaron aislamientos de S. aureus y S. epidermidis meticilino-susceptibles y meticilino-resistentes (SARM, SASR, SERM y SESM). La técnica de susceptibilidad empleada fue el método microdilución en caldo para la detección de la concentración mínima inhibitoria.Resultados. Se identificaron 313 aislamientos de Staphylococcus spp., la mayoría resistentes a meticilina (63.6%). Las cepas SARM y SERM correspondieron al 13.7% y al 27.8% del total de aislamientos, respectivamente. Los mayores porcentajes de resistencia en SARM y SERM correspondieron a eritromicina (57.6% y 81.2%, respectivamente), clindamicina (54.6% y 71.0%), ciprofloxacina (48.4% y 36.4%) y trimetoprima-sulfametoxazol (36.4% y 51.4%).Conclusión. Los resultados encontrados sugieren el replanteamiento de las estrategias de control de la resistencia antimicrobiana en el hospital objeto de estudio.

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Resistencia a antibióticos de bacterias aisladas de biopelículas en una planta de alimentos

Cited by 9 publications

References 14 publications

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

Anti-biofilm activity of ibuprofen and diclofenac against some biofilm producing Escherichia coli and Klebsiella pneumoniae uropathogens

Antimicrobial resistance trends in methicillin-resistant and methicillin-susceptible Staphylococcus aureus and Staphylococcus epidermidisisolates obtained from patients admitted to intensive care units. 2010-2015

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