This study aimed to find out the possible effect of downy mildew infection of lettuce plants in powder form on experimental rats. Thirty adult white male rats , each weighing (110-140 ) g , were used and divided into 5 equal groups (sex rats in each group). The first group is the negative control group. As for the other groups, healthy and infected lettuce leaves were added, used at a rate of 5% and 10% each of the main meal in crushed form. At the end of the experiment, they were anesthetized and blood samples were collected and body weight gain , feed intake, Feed Efficiency Ratio, liver functions (GPT -GOT -ALP), kidney functions (urea -creatinine -uric acid), total protein, (albumin -globulin), blood lipid level (total cholesteroltriglycerides), lipoproteins (HDL-c -LDL-c -VLDL-c) and complete blood count (hemoglobin -hematocrit -platelets -RBCs -WBCs) were evaluated. Histopathological examination of the liver and kidney was also performed. The obtained results concluded that feeding on lettuce leaves led to a significant increase in (hemoglobin, hematocrit, platelets and red blood cells) and a decrease in (albumin, uric acid, urea, creatinine, white blood cells and liver and kidney weight) compared to the negative control group. Also feeding on 5% of infected lettuce leaves gave the highest value in