Resistin Induces Cardiomyocyte Hypertrophy in H9c2 Embryonic Rat Myocardial Cells via Inhibition of MiR-489-3p

Abstract

Background and Objective:The miRNAs play an important regulatory role in resistin-induced cardiac hypertrophy. The purpose of this experiment was to investigate the effect of resistin-induced myocardial hypertrophy in H9c2 cardiomyocytes by inhibiting the expression of miR-489-3p. Materials and Methods: After treatment with resistin or resistin plus miR-489-3p mimic, Image J was used to measure cell surface area. The BCA method and cell counting were used to measure protein synthesis. RT-qPCR was used to measure the relative expression levels of atrial natriuretic factor (ANF), brain natriuretic factor (BNF) and miR-489-3p. Results: Overexpression of the miR-489-3p reduced resistin-induced increase in cardiomyocyte area. Overexpression of miR-489-3p attenuated protein synthesis induced by resistin. Overexpression of miR-489-3p decreased the relative expression of cardiac hypertrophy marker ANF and BNF increased by resistin. Resistin inhibited the expression of miR-489-3p. Conclusion:Resistin induces cardiomyocyte hypertrophy by inhibiting the expression of miR-489-3p. This study provides a theoretical basis for further research on the role of miRNA in resistin-induced cardiomyocyte hypertrophy.