Resistin levels of serum and follicular fluid in non-obese patients with polycystic ovary syndrome during IVF cycles

Abstract

Abstract:Objectives: To measure serum and follicular resistin, steroids hormone levels in women with PCOS (polycystic ovary syndrome) (BMI (body mass index)<25 kg/m 2 ), to assess possible correlations of resistin to hormonal and metabolic parameters and to analyze the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) in women with PCOS and tubal infertility. Study design: We analyzed the clinical outcomes of IVF-ET in women with PCOS (BMI<25 kg/m 2 ) and tubal infertility during the years 2002 to 2004 and compared the serum and follicular fluid resistin levels, estradiol (E 2 ), progesterone (P), testosterone (T) levels in 20 PCOS and 20 healthy, age-matched women without PCOS during IVF-stimulated cycles. The correlations between the resistin levels and the outcomes of IVF-ET were evaluated. Results: No significant differences in resistin levels of either serum or follicular fluid between PCOS and control group were found. However, resistin levels in serum were higher than that in follicular fluid in both groups. Multiple regression analysis showed that resistin levels in serum did not correlate with BMI, estradiol, LH (luteinizing hormone) and insulin level in fasting blood. No significant correlations were found between follicular fluid reisistin levels and fertilization rate, implantation rate, clinical pregnancy rate or early miscarriage rate in both PCOS and control groups. Conclusion: Our results show that resistin does not have correlation with the hormonal and metabolic parameters as well as the outcomes of IVF. These data suggest that resistin is unlikely to be a local determinant factor in steroidogenesis and growth and maturation of oocytes during IVF-ET in lean women with PCOS.

Resistin is a survival factor for porcine ovarian follicular cells

“…doses, in accordance with the plasma resistin concentration in humans (5-15 ng/ml) (Lu et al 2005, Munir et al 2005, Asimakopoulos et al 2009). Moreover, our results are in agreement with an observation in human preadipocytes where resistin did not influence cell proliferation (Ort et al 2005).…”

Estrogen replacement therapy decreases plasma adiponectin but not resistin in postmenopausal women

“…As far as we are aware, the correlations between resistin and estrogen have not been evaluated in postmenopausal women. However, in adult women with polycystic ovary syndrome, serum levels of resistin and estradiol did not correlate significantly [40,41]. The sexual dimorphism in plasma resistin concentrations is probably caused by factors other than estrogens.…”

E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study

“…In contrary, Chen et al [2007] in a case-control study, reported a negative correlation between resistin levels and the number of oocytes retrieved, but similar fertilization rates, in 44 non-PCOS women undergoing IVF; authors suggested a direct link of high levels with poor ovarian response and attributed their findings to the direct effect of inflammatory cytokines on resistin production, cytokines which, per se, were implicated to the etiology of their subfertility [24]. Lu et al (2005), in a retrospective observational study, found no correlation between serum measurement on day 3 before stimulation and FF levels on fertilization, maturation, miscarriage and clinical pregnancy rates in 20 PCOS and 20 non-PCOS women undergoing IVF; authors concluded that resistin does not have any impact in steroidogenesis and oocyte development in this group of subfertile women [26]. Additionally, Seow et al [2005] in a prospective clinical trial, found no correlation between serum and FF resistin levels with pregnancy outcome in PCOS women on IVF [25].…”

“…Treatment allocation was achieved according to the number of the file of each patient (0 to 3 vs. 4 to 9 as a last digit), respectively. In order to avoid potential sources of bias, we included participants who were referred to the Unit consecutively, and the number selected was based on previous reports [24][25][26]. The enrollment of the participants to the two protocols was based on the standard clinical practice of the Unit.…”

“…Animal studies have demonstrated a contributory function in testosterone production and pubertal hypothalamic-pituitary signaling, attributing a role as an endocrine mediator of the reproductive axis [14,17,18] and a potential one concerning obesity-associated insulin resistance [12,19,20]. In reproduction, there has been a controversy on its possible linkage with anovulatory PCOS [21][22][23], or non-PCOS women undergoing IVF [24], further negating the impact of resistin on pregnancy outcome parameters [25,26]. Recent studies have demonstrated the presence of resistin in human granulosa [27], as well as in cumulus, theca cells and oocytes [28].…”