Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles

Kostyuk, A. B.; Vorotnov, Artem D.; Ivanov, A. V.; Volovetskiy, Arthur B; Kruglov, A. V.; Sencha, Ludmila M; Liang, Liuen; Guryev, Evgenii L.; Vodeneev, Vladimir; Deyev, Sergey M.; Lu, Yiqing; Zvyagin, Andrei V.

doi:10.1007/s12274-019-2527-0

Cited by 20 publications

(17 citation statements)

References 42 publications

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“…The femtosecond laser tuned to 975 nm excited endogenous fluorescence alongside the excitation of UCNPs, and hence the image contrast was compromised. Continuous-mode laser excitation can improve the contrast [14], as well as the use of UCNP of the formula β-NaYF 4 :Yb 3+ , Tm 3+ /NaYF 4 with the enhanced conversion efficiency (η UC ) [15]. These advances have the potential for image multiplexing and demultiplexing throughτ UC [16], which can be Note: PLphotoluminescence, UCNPs -upconversion nanoparticles.…”

Section: List Of Abbreviations Mpm -Multi-photon Microscopy Pl -Photo...mentioning

confidence: 99%

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Lifetime imaging of the discrete nanophosphors in biological systems

Zvyagintcev

Yudintsev

Maleki

et al. 2022

jour

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The aim. Demonstrate a novel modality of laser-scanning multiphoton microscopy suitable for rapid acquisition of images of samples labelled with phosphorescent materials characterised by long emission lifetime measured in microseconds. The reported microscopy represents an advancement over the existing laser-scanning modalities, where the acquisition of images of phosphorescent materials takes unpractically long time.Materials and methods. The reported method is based on rapid scanning of the focussed excitation beam across a sample while continuously recording the photoluminescent (PL) signal. The resultant images of discrete phosphorescent nanoparticles appeared blurred. The diffraction-limited image was reconstructed by using a deconvolution algorithm, where the PL lifetime was the key input parameter. To test the method, two types of upconversion nanoparticles (UCNP) were synthesised, NaYF4:Yb3+:Er3+/NaYF4 (E-UCNP), β-NaYF4:Yb3+, Tm3+/NaYF4 (T-UCNP) and used to test a possibility of demultiplexing the two types of UCNPs ex vivo taken up in the mouse liver.Results. The resultant images of E-UCNP, T-UCNP on the background of the liver were fully reconstructed and exhibited the enhanced signal-to-noise ratio. Besides, the method allowed rapid (at the scale of seconds) acquisition of the UCNP PL lifetime and clear discrimination of the two types of UCNPs.Conclusion. We demonstrated a new approach for rapid PL image acquisition of samples containing PL materials, such as biological specimens labelled with discrete UCNPs. Blurred images were shown to be reconstructed at the post-processing stage by applying a deconvolution procedure. This enabled demonstration of multiplexing/demultiplexing using lifetime imaging mode, where the lifetime was engineered by the UCNP synthesis and reconstructed during multiphoton image acquisition using the deconvolution algorithm. The power of this method was demonstrated by the identification of two types of UCNPs accumulated in the liver of a laboratory animal. We believe that the demonstrated method can be useful for rapid lifetime imaging where several molecular specific labelling agents are required.

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Section: List Of Abbreviations Mpm -Multi-photon Microscopy Pl -Photo...mentioning

confidence: 99%

“…To demonstrate the advantages and limitations of the proposed lifetime imaging, we developed a simplified theoretical approach [15].…”

Section: Theoretical Modellingmentioning

confidence: 99%

Lifetime imaging of the discrete nanophosphors in biological systems

Zvyagintcev

Yudintsev

Maleki

et al. 2022

jour

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“…The four-photon 455-nm emission of Tm 3+ is a good paradigm for achieving high-resolution imaging. Denkova (Denkova et al, 2019 ) and Zvyagin (Kostyuk et al, 2019 ) et al realized a ca . 200-nm resolution in heavily doped NaYF 4 :Yb,Tm UCNPs under 980-nm excitation ( Figure 2D ).…”

Section: Perspectives Of Ucnps-based Super-resolution Imagingmentioning

confidence: 99%

Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy

2021

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Super-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real energy levels, lanthanide-doped upconversion nanoparticles are featured by unique optical properties including excellent photostability, large anti-Stokes shifts, multicolor narrowband emissions, tunable emission lifetimes, etc. The past few years have witnessed the development of upconversion nanoparticles as probes for super-resolution imaging studies. To date, the optimal resolution reached 28 nm (λ/36) for single nanoparticles and 82 nm (λ/12) for cytoskeleton structures with upconversion nanoparticles. Compared with conventional probes such as organic dyes and quantum dots, upconversion nanoparticle-related super-resolution microscopy is still in the preliminary stage, and both opportunities and challenges exist. In this perspective article, we summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field. This perspective article should be enlightening for designing efficient upconversion nanoprobes for super-resolution imaging and promote the development of upconversion nanoprobes for cell biology applications.

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“…[112] The UCNPs have also been used to reduce the background autofluorescence and improve the resolution of IVM. [113] Another technique used to perform noninvasive imaging of deep tissue is photoacoustic imaging, an imaging modality in which near infrared laser pulse delivered to the tissues is converted to ultrasonic emission. [114] This imaging modality has been used to detect gastric acid secretion in the stomach.…”

Section: Image Stabilization and Correction Techniquesmentioning

confidence: 99%

Understanding the In Vivo Fate of Advanced Materials by Imaging

Garlin

et al. 2020

Adv Funct Materials

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Engineered materials are ubiquitous in biomedical applications ranging from systemic drug delivery systems to orthopedic implants, and their actions unfold across multiple time‐ and length‐scales. The efficacy and safety of biologics, nanomaterials, and macroscopic implants are all dictated by the same general principles of pharmacology as applied to small molecule drugs, comprising how the body affects materials (pharmacokinetics, PK) and conversely how materials affect the body (pharmacodynamics, PD). Imaging technologies play an increasingly insightful role in monitoring both of these processes, often simultaneously: translational macroscopic imaging modalities such as magnetic resonance imaging and positron emission tomography/computed tomography offer whole‐body quantitation of biodistribution and structural or molecular response, while ex vivo approaches and optical imaging via in vivo (intravital) microscopy reveal behaviors at subcellular resolution. In this review, the authors survey developments in imaging the in situ behavior of systemically and locally administered materials, with a particular focus on using microscopy to understand transport, target engagement, and downstream host responses at a single‐cell level. The themes of microenvironmental influence, controlled drug release, on‐target molecular action, and immune response, especially as mediated by macrophages and other myeloid cells, are examined. Finally, the future directions of how new imaging technologies may propel efficient clinical translation of next‐generation therapeutics and medical devices are proposed.

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Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles

Cited by 20 publications

References 42 publications

Lifetime imaging of the discrete nanophosphors in biological systems

Lifetime imaging of the discrete nanophosphors in biological systems

Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy

Understanding the In Vivo Fate of Advanced Materials by Imaging

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