Resolution and isolation of avian and amphibian yolk-granule proteins using TEAE-cellulose

The purification and partial characterization of carp, Cyprinus carpio, vitellogenin

“…A number of procedures have been developed for the isolation of intact VTG from amphibian and avian species (Ansari et al 1979;Redshaw and Follett 1971;Wallace 1965;Wiley et al 1979). However, these methods have proved less successful in their application to the isolation of teleost VTG(s).…”

Vitellogenesis and Oocyte Growth in Nonmammalian Vertebrates

“…The granule fraction, approximately 20% of the yolk solids, contains two lipovitellins (a and ~) in equal amounts, phosvitin, and a variable amount of low-density lipoprotein, which is most likely a contaminant from the supernatant material (Burley and Cook, 1961). The native (dimer) a-and ~-lipovitellins seem to have a similar size (-400,000 M" Bernardi and Cook, 1960b) and lipid distribution, primarily phospholipid (Martin et 01., 1963), but differ from one another in electrophoretic migration (Bernardi and Cook, 1960a), chromatographic behavior (Burley and Cook, 1961), dissociative behavior (Burley and Cook, 1962), protein-phosphorus content (Wallace, 1965), and polypeptide composition (Bergink et 01.,1974). Partial proteolysis experiments have indicated that vitellogenin II gives rise to polypeptides in both a-and ~ lipovitellin, whereas vitellogenin I gives rise to only a-lipovitellin polypeptides (Wang and Williams, 1980).…”

“…In their native state, lipovitellins are large lipoprotein dimers (-400,000 Mr) that dissociate into monomeric subunits (-200,000 Mr) under alkaline conditions (Wallace, 1965). By contrast, phosvitins are relatively small phosphoproteins (30,000-40,000 Mr ), which are highly phosphorylated (-10% protein-phosphorus) and in which blocks of serine represent about one-half the total amino acids (Taborsky, 1974;Wallace, 1978).…”

“…The water-insoluble platelets from anamniotic eggs or the granules from bird eggs can be dissolved in high-ionic-strength solutions, yielding two types of yolk protein, the lipovitellins and phosvitins; these can be separated from one another by cellulose chromatography or ammonium sulfate fractionation (Wallace, 1965;Wallace et al, 1966). In their native state, lipovitellins are large lipoprotein dimers (-400,000 Mr) that dissociate into monomeric subunits (-200,000 Mr) under alkaline conditions (Wallace, 1965).…”

Dehydration induced interactions of egg yolk lipoproteins and low molecular weight carbohydrates

“…The molecular weight was revised upward to 450,000 by Cook'and Wallace (1965). With methods currently employed, Wallace (1965) reported that the lipid content of the lipovitellins can only be established as being between l6 and 22fo.…”