Resolution Behavior of cis- and trans-Octadecenoic Acid Isomers by AOCS Official Method Using SP-2560 Column

Yoshinaga, Kazuaki; Asanuma, Masaharu; Xu, Chao; Mizobe, Hoyo; Kojima, Kensuke; Nagai, Toshiharu; Beppu, Fumiaki; Gotoh, Naohiro

doi:10.5650/jos.62.781

Cited by 12 publications

(4 citation statements)

References 12 publications

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“…× 0.20-μm thickness; Bruker), flame ionization detector, and Compass CDS software (Bruker). Fatty acid identification was analyzed using the GLC-532, GLC-401, GLC-643, GLC-642, GLC-463, C18:1 t11, C19:0, C23:0 standard references (Nu-Chek-Prep Inc., Elysian, MN, USA) and the relative retention times according to what was observed in the bibliography (Alves et al, 2009, Lee et al, 2012Yoshinaga, 2013;Bravo-Lamas et al, 2016). FA quantification was performed as described in UNE-EN 12,966-4 Official Method (2015).…”

Section: Carcass Measurements and Meat Analysismentioning

confidence: 99%

Tropical grass and legume pastures may alter lamb meat physical and chemical characteristics

Hampel

Poli

Joy

et al. 2021

Trop Anim Health Prod

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The present study assessed the influence of the type of the tropical pastures on lamb body weight (BW) gain and meat quality. Fifty-four lambs were allocated to three grazing pastures: (1) AG -Aruana grass (Panicum maximum cv. IZ-5); (2) PPpigeon pea legume (Cajanus cajan cv. Anão); and (3) CS -contiguous swards, half of the paddock with AG and half with PP. After 92 days of grazing, the lambs were slaughtered. Carcasses were evaluated and the longissimus muscle was collected to determine color, lipid profile, tocopherol concentrations, and lipid oxidation. Although the pastures present differences in the characteristics of nutritional quality, the animals did not show difference in BW gain. The results show that all forage presented similar concentration of alpha-tocopherol (137 ± 14.37 mg kg −1 of fresh matter), whereas total and condensed tannin contents were greater in PP, intermediate in CS, and the lowest in AG treatment (P = 0.0001). Meat α-tocopherol content was similar among treatments (P = 0.1392), with an average concentration close to the optimal level to reduce the meat oxidation. Meat from AG treatment had 45 and 25% lower n-6/n-3 ratio than meat from PP and CS treatments, respectively. The legume increases the unsaturated fatty acids and the grass can reduce the n6/n3 ratio. The level of condensed tannin concentration did show to have important effect on meat characteristics. Both tropical pastures studied can provide a high amount of alpha-tocopherol, generating a great potential to increase the concentration of this antioxidant in lamb's meat.

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Section: Carcass Measurements and Meat Analysismentioning

confidence: 99%

Tropical grass and legume pastures may alter lamb meat physical and chemical characteristics

Hampel

Poli

Joy

et al. 2021

Trop Anim Health Prod

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“…It was noticed that an oven temperature of ~180°C gave better resolution among FAMEs with <C18, and an oven temperature of ~210°C gave better resolution among FAMEs with >C18. Separation behaviors of cis- and trans-isomers of C18:1 are greatly affected by column and oven temperatures ( 28 , 29 ). Thus, the column temperature was divided into three temperature change stages by setting an appropriate rate of increase in the oven temperature to achieve baseline separation of most FAMEs in the total ion chromatogram (TIC; Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

Updating the fatty acid profiles of retail bovine milk in China based on an improved GC-MS method: implications for nutrition

Chen

Wang

et al. 2023

Front. Nutr.

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The importance of food components to potential benefits and risks to human health is gradually being consumer awareness. Milk is an important part of the lipid content of the human diet, and there are few detailed reports on the fatty acid (FA) profiles of retail milk. In the study, we developed a gas chromatography–mass spectrometry (GC-MS) method to simultaneously determine 82 FAs, including 11 even-chain saturated FAs, 10 odd-chain saturated FAs, 9 branched-chain saturated FAs, 30 monounsaturated FAs, and 22 polyunsaturated FAs; this was applied to analyze samples (186 samples) of commercially available milk from 22 provinces throughout China and to evaluate the nutritional value of these samples based on FA-related indices. The results showed that the overall composition of milk FAs among the different regions was numerically similar, and minor FAs showed few differences. When considering the retail milk FA composition and dairy fat intake in China, regional variations have a limited impact on FA consumption. Moreover, milk accounts for approximately one-third and <10% of the maximum recommended intake of saturated FAs and trans-FAs in consumer diets, respectively. This study provides an updated report on the composition of FAs and the nutritional value of retail milk across China, which can serve as a reference for producers for future research on regulating milk FAs, for consumers to select milk, and for nutrition departments to formulate relevant nutritional guidance recommendations.

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“…As GC‐MS is superior to GC in terms of analyte selectivity based on the mass, so GC‐MS has the advantage in the analysis of FAME, especially in the presence of matrix background. In the conventional GC procedure, relative response factors for target analytes should be determined in advance, but each TOA isomer has its own response factor, although the difference might be small . By contrast, in GC‐MS, the detector responses of fragment ions derived from different positional isomers of monounsaturated FAME are, in principle, essentially the same, because the rearrangement of double bonds occurring upon ionization produces the same fragments from each isomer .…”

Section: Resultsmentioning

confidence: 99%

Trans fatty acid accumulation in the human placenta

Wada¹,

Yoshida-Yamamoto

Wada

et al. 2017

J. Mass Spectrom.

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Trans fatty acid may impair fetal growth and infant neurodevelopment, but the quantity in a placenta and human tissues remains unknown. To address the issue, a simple and reliable method of quantification is needed. We established a method of quantifying trans-octadecenoic acids (trans-6,8,9,11 18:1 fatty acids, TOAs), a major component of trans fatty acid, in human tissue samples, and then determined the TOAs level in the placenta. Oleic acid (OA) (C18:1(9c)) was measured by isotope dilution gas chromatography-mass spectrometry, and the TOAs level was subsequently calculated based on the ratio of the peak areas for TOAs and OA (TOAs/OA) in the mass chromatogram. Lipids were extracted from 28 human placentas at different gestational ages from 28 to 41 weeks, and the TOAs and OA levels were measured. In method validation, the limit of detection for elaidic acid (trans-9,18:1 fatty acid), a major component of TOAs, was 0.57 ng, and linearity of calibration ranging from 7.7 to 68.0 μg/g placenta for TOAs. In human placenta analysis, the TOAs level was significantly higher in term (n = 15, 40.2 ± 9.7 μg/g placenta) than in preterm placentas (n = 13, 18.9 ± 7.4 μg/g placenta) (p < 0.001), while OA levels were similar in term (n = 15, 863 ± 132 μg/g placenta) and preterm (n = 13, 743 ± 283 μg/g placenta) placentas (p = 0.15). TOAs accumulate in the placenta as pregnancy progresses and have a fate different from that of OA in vivo. To our knowledge, this is the first report of TOA quantification in human tissue samples. Copyright © 2017 John Wiley & Sons, Ltd.

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Resolution Behavior of cis- and trans-Octadecenoic Acid Isomers by AOCS Official Method Using SP-2560 Column

Cited by 12 publications

References 12 publications

Tropical grass and legume pastures may alter lamb meat physical and chemical characteristics

Tropical grass and legume pastures may alter lamb meat physical and chemical characteristics

Updating the fatty acid profiles of retail bovine milk in China based on an improved GC-MS method: implications for nutrition

Trans fatty acid accumulation in the human placenta

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