Resolution enhancement in three-dimensional confocal microscopy

Hewlett, S. J.; Wilson, Tony

doi:10.1007/bf01815300

Cited by 32 publications

(12 citation statements)

References 8 publications

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“…By removing predominantly multiply scattered light, the difference image has a narrower confocal point spread function, resulting in greater image contrast. These results are consistent with Hewlett and Wilson’s subtractive confocal imaging work 18 .…”

Section: Resultssupporting

confidence: 90%

Non-mydriatic confocal retinal imaging using a digital light projector

Muller

Elsner

Ozawa

2013

Ophthalmic Technologies XXIII

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A digital light projector is implemented as an integrated illumination source and scanning element in a confocal non-mydriatic retinal camera, the DLP-Cam. To simulate scanning, a series of illumination lines are rapidly projected on the retina. The backscattered light is imaged onto a 2-dimensional rolling shutter CMOS sensor. By temporally and spatially overlapping the illumination lines with the rolling shutter, confocal imaging is achieved. This approach enables a low cost, flexible, and robust design with a small footprint. Qualitative image comparison with commercial non-mydriatic SLOs and fundus cameras shows comparable fine vessel visibility and contrast.

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Section: Resultssupporting

confidence: 90%

Non-mydriatic confocal retinal imaging using a digital light projector

Muller

Elsner

Ozawa

2013

Ophthalmic Technologies XXIII

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“…Some negative intensity values will inevitably appear in the difference image after subtraction. We simply exclude these negative intensities from the image to improve the imaging quality21.…”

Section: Resultsmentioning

confidence: 99%

Breaking the Diffraction Barrier Using Fluorescence Emission Difference Microscopy

Kuang

Liu

et al. 2013

Sci Rep

142

103

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We propose a novel physical mechanism for breaking the diffraction barrier in the far field. Termed fluorescence emission difference microscopy (FED), our approach is based on the intensity difference between two differently acquired images. When fluorescence saturation is applied, the resolving ability of FED can be further enhanced. A detailed theoretical analysis and a series of simulation tests are performed. The validity of FED in practical use is demonstrated by experiments on fluorescent nanoparticles and biological cells in which a spatial resolution of <λ/4 is achieved. Featuring the potential to realize a high imaging speed, this approach may be widely applied in nanoscale investigations.

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“…Some negative intensity values will inevitably appear in the difference image, and these negative intensities are simply excluded from the image to improve the imaging quality [32]. The excitation PSF of the FED method can be written as…”

Section: Theorymentioning

confidence: 99%

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy

Wang

Kuang

et al. 2015

Appl. Opt.

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Dual-mode super-resolution imaging system with two different super-resolution imaging methods, STED and FED, is presented. Electrical shutters controlled by the host computer are introduced to switch the two imaging modes. Principles of both methods are analyzed theoretically, and enhancements in the lateral resolution and SNR are demonstrated theoretically and experimentally. Results show that both imaging methods offered by the proposed system can break the diffraction barrier. Furthermore, the presented system provides a meaningful way to image fluorescent samples by a corresponding imaging mode according to the specific characteristics of samples analyzed for study. For samples that can endure high-power illumination, it is appropriate to use the STED mode to achieve a better resolution, while for samples that are vulnerable to high intensity, the FED method is a better choice because no high-power beam is needed, and the FED method can provide better resolution than STED when no high-power beam is allowed. The flexible switching of the two super-resolution imaging modes can help researchers to make most of the advantages of each imaging method. It is believed that the presented system has the potential to be widely used in future nanoscale investigations.

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Resolution enhancement in three-dimensional confocal microscopy

Cited by 32 publications

References 8 publications

Non-mydriatic confocal retinal imaging using a digital light projector

Non-mydriatic confocal retinal imaging using a digital light projector

Breaking the Diffraction Barrier Using Fluorescence Emission Difference Microscopy

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy

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