Resolution of amine enantiomers using precolumn derivatization with dansyl-L-proline and reversed-phase liquid chromatography

Enantiomeric separation of chiral amines in biological samples by liquid chromatography with chiral fluorous derivatization

“…The data comparing the retention time and resolution between the present chiral derivatization and the previous chiral derivatization of PEA [9, 11] are shown in Table 3. Both retention time and resolution were superior to those of the chiral derivatization by Mochizuki et al .…”

“…The data comparing the retention time and resolution between the present chiral derivatization and the previous chiral derivatization of PEA [9,11] are shown in Table 3. Both retention time and resolution were superior to those of the chiral derivatization by Mochizuki et al However, the present chiral derivatization saves pretreatment time because the sample matrix can be avoided by LC separation, and the resolution was sufficient for quantitative analysis.…”

“…Various chiral LC stationary phases have been used for the analysis of enantiomer compounds, and chiral derivatization has been developed as an alternative method because of its low cost and scalability [2]. Currently, various chiral derivatization reagents have been reported [3][4][5][6][7][8][9][10][11][12][13][14]. Some of F I G U R E 1 Derivatization of PEA using fluorous chiral derivatization reagents these reagents have certain advantages that aid detection; for example, some of these enable not only enantiomer separation but also UV [3][4][5][6] and fluorescence detection [7][8][9][10], and improve ionization efficiency in MS [11][12][13][14][15][16].…”

“…Currently, various chiral derivatization reagents have been reported [3][4][5][6][7][8][9][10][11][12][13][14]. Some of F I G U R E 1 Derivatization of PEA using fluorous chiral derivatization reagents these reagents have certain advantages that aid detection; for example, some of these enable not only enantiomer separation but also UV [3][4][5][6] and fluorescence detection [7][8][9][10], and improve ionization efficiency in MS [11][12][13][14][15][16]. These reagents are very useful because they can afford both enantiomeric separation and improved detection sensitivity simply via derivatization.…”

N-[4-(6-Methoxy-2-benzoxazolyl)]benzoyl-l-amino Acids as Chiral Derivatization Reagents for Amine Enantiomers

“…This result shows that the sensitivity of BOX-L-Pro is better than that of benoxaprofen chloride with fluorescence detection25, and almost the same as that of dansyl-L-proline with fluorescence detection. 10 A calibration graph of peak area versus concentration of R-NEA labeled with BOX-L-Pro was plotted under reversed-phase mode. A good linear detector response (linear regression coefficient=0.999) was observed in the range of 1-200 pmol R-NEA, injected on-column.…”