Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples

Eiroa, A. Andrade; Blanco, E. Vázquez; López-Mahı́a, Purificación; Muniategui-Lorenzo, Soledad; Prada‐Rodríguez, D.

doi:10.1039/b001488p

Cited by 20 publications

(4 citation statements)

References 19 publications

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“…The final concentration of CTAB in the test solution was maintained at 7.8× 3 10 − mol/L as reported in the Ref. [11].…”

Section: Reagentsmentioning

confidence: 99%

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Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

Zhang

2007

Wuhan Univ. J. of Nat. Sci.

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A sensitive analytical method, constant-energy synchronous fluorimetry, has been used to determine benzo[a]pyrene (BaP) selectively in airborne particulates (AP). A constant-energy difference of 1 400 cm -1 was selected for obtaining single spectral peak. The method could easily identify BaP in an 18-component polycylic aromatic hydrocarbons(PAHs) mixtures. Calibration plots in methanol solution and in sodium dodecylsulfate (SDS) micellar media show a good linearity (R>0.999) in the BaP concentration range generally found in the environment. The method gives a detection limit of 0.40 nmol/L in SDS micellar medium and 1.34 nmol/L in methanol solution.

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“…The final concentration of CTAB in the test solution was maintained at 7.8× 3 10 − mol/L as reported in the Ref. [11].…”

Section: Reagentsmentioning

confidence: 99%

“…This complication can be overcome by using special techniques such as synchronous spectrometry. Constant-energy synchronous luminescence spectrometry (CESLS) [9,10] , one important embranchment, has been successfully applied to BaP identification in PAHs mixture [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

Zhang

2007

Wuhan Univ. J. of Nat. Sci.

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“…Thus, a simple and narrower spectrum is obtained in SFS compared to conventional fluorescence measurements. This technique has been successfully applied to multi-component analysis (7)(8)(9)(10)(11)(12) and petroleum products analysis (13)(14)(15) and also to analyse PAH adducts (16)(17)(18)(19) and for the detection of metabolites of PAHs in human urine (20).…”

Section: Introductionmentioning

confidence: 99%

Simple luminescence method for estimation of benzo[a]pyrene in a complex mixture of polycyclic aromatic hydrocarbons without a pre‐separation procedure

Patra

2003

Luminescence

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Benzo[a]pyrene causes cancer at cellular level and is widely present in the environment. Conventional spectroscopic methods for analysis of this compound need a pre-separation procedure due to severe spectral overlap from other polycyclic aromatic hydrocarbons. We report a simple method that avoids spectral overlap of benzo[a]pyrene from other impurities or polycyclic aromatic hydrocarbons (PAHs), thus it can easily identify benzo[a]pyrene in a complex PAH mixture. The method could easily identify benzo[a]pyrene in an 18-component PAH mixture. Calibration plots in methanol solution and in micellar media show a good linearity (R > 0.9997) in the benzo[a]pyrene concentration range generally found in the environment. The method gives a detection limit of 1.52 x 10(-9) mol/L in CTAB micellar medium and 2.55 x 10(-9) mol/L in methanol solution. The proposed method is selective, sensitive and fast. The fluorescence response of benzo[a]pyrene is found to be a potential candidate to sense the critical micellar concentration (CMC) of CTAB micelles.

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“…This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS.…”

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confidence: 99%

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

Lin

2005

ANAL. SCI.

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The identification and quantification of polycyclic aromatic hydrocarbons (PAHs) in the environment are of great interest due to their carcinogenic and mutagenic characteristics. 1 Chromatographic methods, like GC-MS, HPLC with UV-visible or fluorescence detector etc., are time-consuming, expensive and require a separation procedure before analysis. Because of the strong intrinsic fluorescence emission of PAHs, fluorescence spectroscopy could provide a highly sensitive method for the analysis of PAHs.Synchronous fluorescence spectroscopy (SFS) has been known to be a simple and selective method for the simultaneous determination of multicomponent samples, 2-6 while maintainning the fluorometric sensitivity.Inman and Winefordner 7,8 described a kind of synchronous scanning mode, namely constant-energy synchronous luminescence spectrometry (CESLS), in which a constant energy difference (∆ -υ) between excitation and emission monochromators is maintained as each is scanned through the spectral region of interest. This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS. The fluorescence excitation and emission spectra of PAHs are generally characterized with similar spaced vibrational features. CESLS can attain the same spectral features with a single scan as conventional constant-wavelength synchronous fluorescence spectroscopy in PAH multicomponent analysis.However, resonance electronic energy transfer in mixture of PAHs has limited the application of SFS for PAH analysis. 19 For example, there is appreciable energy transfer from pyrene to perylene in a solution containing both. 20,21 The micellization of a PAHs mixture solution can help resolve this problem by isolating fluorophores in different micelles. 22,23 Micelles can also easily solubilize PAHs and increase the fluorescence intensities of fluorophores, thereby increasing the sensitivity. The analysis of certain PAHs mixtures in micellar media has been made using conventional fluorometric techniques, 22,24,25 as well as constant-wavelength and variable-angle synchronous fluorometry. 23,26,27 It can be expected that CESLS would encounter the same problem of energy transfer, and micelles would be a good solution, as in other fluorometric methods.In this paper, combined use of micellar media and constantenergy synchronous fluorescence spectroscopy for the simultaneous determination of pyrene, perylene and benzo [a]pyrene in water samples is described.To our knowledge, this is the first report on the application of micellar media in CESLS. The method exempted the resonance electronic energy transfer among the different PAHs, and thus allowed their simultaneous identification and quantitative determination via a single spectrum. Because th...

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Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples

Cited by 20 publications

References 19 publications

Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

Simple synchronous fluorimetric method for determination of benzo[a]pyrene in airborne particulates without a pre-separation procedure

Simple luminescence method for estimation of benzo[a]pyrene in a complex mixture of polycyclic aromatic hydrocarbons without a pre‐separation procedure

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

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