Resolution of ribosomal complexes and RNA isolated from bacterial and mammalian sources

Changes in the ribonucleic acid metabolism of aging mouse tissues with particular reference to the prostate gland

“…The citric acid-washing procz -@ cedure should strip the lysine-rich histone from DNA (Bonner & Huang, 1966b e of the general distribution was needed. The profile o of the RNP particles from the livers of young mice was similar to the distribution of liver polysomes reported by Wettstein et al (1963) andWebb, Blobel &Potter (1964), but differed significantly from the results of Murthy (1966) and Abell et al (1967), who found that the bulk of liver RNP particles remained at the top of the gradients. A comparison of results from different Laboratories is often difficult, and in this case the disparities may be due to different methods of preparation and such factors as the temperatures at which the various procedures were conducted (Wettstein et al 1963).…”

“…(a) RNP particles. The RNP suspensions were examined in a system essentially based on that of Abell, Rosini & Ramseur (1967). In the present work, however, the gradients were made up manually and scaled down to an overall volume of 4-75 ml.…”

Resolution and Identification of Ribosomes from Mycobacteria

“…Several methods of isolation of ribosomal complexes from mycobacteria based mainly on differential centrifugation of disrupted bacilli have been reported, and their morphological as well as biochemical properties have been studied (2, 7, 10, 20, 23-28; M. Yoneda, personal communication). Little attention has been given to the detailed study of the individual components (units, subunits, or polyribosomes) which are well known in ribosomes from sources other than mycobacteria (1,12,14,16,17).…”