Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods

Abstract

Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.

A novel eco‐friendly methods for simultaneous determination of aspirin, clopidogrel, and atorvastatin or rosuvastatin in their fixed‐dose combination using chemometric techniques and artificial neural networks

“…Upon the literature survey, a few spectrophotometric methods have been proposed for the analysis of ASP, CLP, and ATV or ROS in pharmaceutical preparations 11–16 . Besides, few liquid chromatography with tandem mass spectrometry (LC‐MS/MS) and high‐performance liquid chromatography (HPLC) methods have been proposed for the analysis of ASP, CLP, and ATV or ROS in pharmaceutical preparations 17–21 …”

“…10 Upon the literature survey, a few spectrophotometric methods have been proposed for the analysis of ASP, CLP, and ATV or ROS in pharmaceutical preparations. [11][12][13][14][15][16] Besides, few liquid chromatography with tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography (HPLC) methods have been proposed for the analysis of ASP, CLP, and ATV or ROS in pharmaceutical preparations. [17][18][19][20][21] The UV absorption spectra of ASP, CLP, and ATV in mixture (1) and ASP, CLP, and ROS in mixture (2) in distilled water at their nominal concentrations ratio in tablets and capsules show strong overlap (Figures 2 and 3).…”

Formulation, method development and validation of water soluble vitamins B1, B2 & B6 in bulk and tablet dosage form by HPTLC method

“…Few reports on spectrophotometric methods are also available. [13][14] Till date there is no report available on High-Performance Thin-Layer Chromatographic (HPTLC) method for the estimation of vitamin B1, B2 and B6…”

Micellar High Performance Liquid Chromatographic Determination of a Binary Mixture of Rivaroxaban and Clopidogrel and Application to Biological Fluids

“…CLP is official in the United State Pharmacopoeia (USP) (3). Literature survey revealed that many HPLC methods have been published for its determination either alone [18][19][20][21] or in combination with other drugs [22][23][24][25][26]. Assay of synthetic mixture of RIV and CLP has been described previously using HPLC method with UV detection at 240 nm [17], but it could not be used for determination of rivaroxaban in plasma.…”