Resolution of the light-harvesting chlorophyll ab-protein of Vicia faba chloroplasts into two different chlorophyll-protein complexes

The chloroplast ATP synthetase consist of the subunits α, β, γ, δ, ϵ and proteolipid only

“…The antibodies raised against I (MI 20000) and II (Mr 17000) combined also with only a single component of the gel electrophoretic thylakoid polypeptide spectrum but their A4, was estimated to be 26000 and 24500, respectively. The first one has been identified as the subunit a of the LHCP complex [2,15,17], and no further component became labeled. Because the result was still explainable by a subunit-specific proteolysis of the components I and II during thylakoid membrane extraction with Triton X-100 A and autoradiography as in [13].…”

Chlorophyll-proteins of thylakoids from wild-type and mutants of barley (Hordeum vulgare L.)

“…It is difficult to make a direct correlation between the bands in the two different gels, but it seems probable that the major band in system II has been resolved into two bands in the urea system. There is indirect evidence from Vicia faba that Chla/b-P2 consists of two distinct apoproteins and hence two chlorophyll-proteins (23). The chlorophyll a/b ratio decreases as one moves down the Chla/b-P2 band, but the relatively high chlorophyll a/b ratio of the free chlorophyll ( Figure 3) could mean that there is a selective loss of chlorophyll a from Chla/b-P2 during electrophoresis.…”

“…A slab-gel apparatus, modified from the design of S+VDIER (32) was used for all three gel types. The components of the Trisglycine/Tris-sulphate gel (system IV) are given in Table II, and those for systems II and III are as previously described (23).…”

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

“…CP29 was first resolved by Machold and coworkers (Machold and Meister, 1979; and later by other groups . In most gel systems a single apoprotein is resolved from CP29 migrating above LHCII apoproteins , but two closely migrating bands have also been described (White and Green, 1987a;Barbato et al, 1989).…”