Resolution of triglycerides in capillary SFC as a function of column temperature

Proot, M.; Sandra, Pat; Geeraert, E.

doi:10.1002/jhrc.1240090313

Cited by 27 publications

(8 citation statements)

References 8 publications

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“…Laakso [209] has reviewed the analysis of lipids by SFC. Many authors described the use of capillary columns for the analysis of TGs by SFC [210][211][212][213][214][215]. All those studies came to the conclusion that even long-chain high-molecular mass TGs can be eluted at moderate temperatures (,150 7C), thus eliminating the problem of thermal degradation usually associated with high temperature GLC of TG.…”

Section: Supercritical Fluid Chromatographymentioning

confidence: 99%

Triacylglycerol profiling by using chromatographic techniques

Buchgraber

Ulberth

Emons

et al. 2004

Euro J Lipid Sci & Tech

189

129

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Triacylglycerol profiling by using chromatographic techniquesTriacylglycerols (TGs) make up the major part of naturally occurring fats and oils. The composition and fine structure of TGs determine to a large extent the functionality of fats and oils as food ingredients and the physiological effects of fats and oils as component of the human diet. Analysis of intact TGs is usually performed by chromatographic methods. In this article the application of gas-liquid chromatography, highperformance chromatography in normal and reversed phase mode, thin-layer chromatography and supercritical fluid chromatography for the qualitative and quantitative determination of TGs is reviewed. Emphasis is put on those factors that are decisive for obtaining reliable quantitative data. Furthermore, techniques for the stereospecific analysis of the fatty acid distribution along the glycerol backbone of TGs are presented briefly.

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Section: Supercritical Fluid Chromatographymentioning

confidence: 99%

Triacylglycerol profiling by using chromatographic techniques

Buchgraber

Ulberth

Emons

et al. 2004

Euro J Lipid Sci & Tech

189

129

View full text Add to dashboard Cite

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“…A separation according to carbon number was obtained. Separation of TG according to carbon number has been demonstrated with nonpolar open tubular columns in SFC [28]. In the present work, some separation of saturated TG having identical carbon numbers was observed as well (c. $ the two peaks eluting right after the main peak at carbon number 44).…”

Section: Resultsmentioning

confidence: 57%

Miniaturized light scattering detector for packed capillary supercritical fluid chromatography

Demirbüker

Anderson

Blomberg

1993

J Microcolumn Separations

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Abstract. Packed capillary columns provide excellent separation of neutral lipids by supercritical fluid chromatography. For these separations, it is necessary to add a polar modifier to the mobile phase; hitherto, a detector suitable for quantitative analysis has been lacking. For this purpose, a miniaturized evaporative light scattering detector for combination with packed microcolumns in supercritical fluid chromatography has been developed. Since the detector response is dependent on the mobile phase flow rate, a drop in response was found at flow rates above 16 mL min-' of expanded mobile phase. However, below this flow rate, there was a region of relatively constant response. In this region, quantitative analysis could also be successfully performed when the pressure and/or the temperature of the separation were programmed. The detection limit was lower than 6 ng. With pressure programming, the relative standard deviation in peak area was less than 4 %. Minor peaks gave higher RSD values.Further, it is demonstrated that argentation SFC of triacylglycerols provides separation according to chain length as well as to the degree of unsaturation.

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“…The resolution at a given temperature was not significantly altered when pressure programming (Method A) was employed instead of density programming (Method B) (data not shown). Others (8,20) also obtained improved resolution of TG when the isothermal column temperature was raised to 150~ but temperatures above 150~ did not improve resolution further. We examined simultaneous pressure and temperature programming by using each a positive (Method C) and a negative (Method D) temperature program.…”

Section: Resultsmentioning

confidence: 99%

Supercritical fluid chromatographic analysis of new crop seed oils and their reactions

Hayes

Kleiman

1996

J Americ Oil Chem Soc

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Supercritica/fluid chromatography (SFC) with an open tubular column of nonpolar stationary phase separated triglycerides from crambe, meadowfoam, Euphorbia lagascae, and vernonia oils based on their molecular weight. The triglyceride compositions were consistent with the literature. SFC proved also to be a valuable tool in analyzing lipase-catalyzed transesterification reactions where lesquerella oil and estolides were among the substrates employed. Analyte molecular weights could be estimated from a retention time-(or elution density-) molecular weight calibration curve. An increase in isothermal column temperature during SFC pressure or density programming improved the resolution of high-molecular-weight (>600 Da) analytes but yielded poorer resolution for analytes of molecular weight < 200. A simultaneous pressure and temperature ramping program proved superior in enhancing resolution in several instances.Supercritical fluid chromatography (SFC) is becoming a valuable tool in the analysis of lipids as it is replacing high-performance liquid chromatography (HPLC) and gas chromatography (GC) as the analytical tool of choice for several applications. SFC can be considered a hybrid of GC and HPLC because supercritical fluids possess properties (e.g., density, viscosity, and self-diffusion coefficient) intermediate between gases and liquids. For example, separation of analytes by relative volatility, as encountered in GC, can also be achieved with SFC but at much lower elution temperatures and without derivatization. The use of lower temperatures protects analytes that are susceptible to thermal degradation, such as polyunsaturated and oxygenated lipids. With the onset of equipment that allows addition of modifiers to the supercritical fluid mobile phase, HPLC-Iike separations can be achieved by SFC, but a larger range of detectors--GC-style

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Resolution of triglycerides in capillary SFC as a function of column temperature

Cited by 27 publications

References 8 publications

Triacylglycerol profiling by using chromatographic techniques

Triacylglycerol profiling by using chromatographic techniques

Miniaturized light scattering detector for packed capillary supercritical fluid chromatography

Supercritical fluid chromatographic analysis of new crop seed oils and their reactions

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