Resolving the phylogenetic relationship of Himalayan snow trout Schizothorax plagiostomus with other species of Schizothoracine using mitochondrial CO-I and Cyt b genes

Purohit, Shriya; Sharma, Partigya; Kothiyal, Sachin; Singh, Upendra; Nautiyal, Prakash; Singh, Mahender; Bhattacharya, Indrashis

doi:10.1007/s11033-023-08274-y

Cited by 1 publication

(4 citation statements)

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“…Therefore, all collected fish samples were screened/identified as per previously reported features (Day, 1888;Mir et al, 2013;Pandey & Nautiyal, 1997) and subsequently were authenticated/verified by molecular markerÀbased analyses. mt genes CO-I and Cyt-b are dependable markers and extensively employed for species identification of teleosts (Singh et al, 2012;Ward et al, 2005) including Schizothoracine species (Ma et al, 2020;Purohit et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, all collected fish samples were screened/identified as per previously reported features (Day, 1888; Mir et al ., 2013; Pandey & Nautiyal, 1997) and subsequently were authenticated/verified by molecular marker−based analyses. mt genes CO‐I and Cyt‐b are dependable markers and extensively employed for species identification of teleosts (Singh et al ., 2012; Ward et al ., 2005) including Schizothoracine species (Ma et al ., 2020; Purohit et al ., 2023). The sequences for CO‐I (707 bp) and Cyt‐b (1142 bp) of the samples confirmed the authenticity of the species identity (as S. plagiostomus ) due to comparable matches with the reference sequence at NCBI GenBank (accession number NC023531).…”

Section: Discussionmentioning

confidence: 99%

“…The mt markers, Cyt‐b (1142 bp) and partial CO‐I (707 bp), were sequenced from adult female tissues (liver/fin/muscle) by the Sanger method using an automated DNA sequencer (ABI‐3500 Genetic Analyzer) at ICAR‐National Bureau of Fish Genetic Resources, Lucknow, India, as per previous protocols (Purohit et al ., 2023; Sanger et al ., 1977). The consensus sequences of all samples were aligned using Clustal‐W, MEGA 7 software (Tamura et al ., 2013) and then blasted at NCBI for the closest sequences and further matched with the authenticated reference of S. plagiostomus deposited at NCBI GenBank.…”

Section: Methodsmentioning

confidence: 99%

“…The mt markers, Cyt-b (1142 bp) and partial CO-I (707 bp), were sequenced from adult female tissues (liver/fin/muscle) by the Sanger method using an automated DNA sequencer (ABI-3500 Genetic Analyzer) at ICAR-National Bureau of Fish Genetic Resources, Lucknow, India, as per previous protocols (Purohit et al, 2023;Sanger et al, 1977).…”

Section: Identification Of Fish Speciesmentioning

confidence: 99%

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A study on the seasonal cyclicity of ovarian development in adult Himalayan snow trout, Schizothorax plagiostomus

Sharma

Purohit

Bhatt

et al. 2023

Journal of Fish Biology

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Schizothorax plagiostomus, commonly known as snow trout, is a popular food fish found in parts of Central Asia and the Indo‐Himalayan region. Despite such a broad range of distribution and potential financial value, it is a highly neglected cold‐water ichthyofauna. Furthermore, an alarming decline in Schizothoracine population has been reported in the recent past due to climate change and uncontrolled anthropogenic interference. In this study, the seasonal variations in ovarian architecture and development were examined in adult S. plagiostomus from Garhwal Himalayan region, Uttarakhand, India. Ovarian‐somatic index ranged from 16.86 ± 0.29 to 0.31 ± 0.56, with a maximal value in September and a minimal value in April. Ovarian histology revealed the abundance of primary growth oocytes in resting and preparatory stages; primary/secondary vitellogenic oocytes with numerous cortical alveoli were predominant in the developing stage of pre‐spawning ovaries; secondary/tertiary vitellogenic oocytes were conspicuous in actively spawning ovaries; and atretic follicles/oocytes were discernible during the regressing stage of spent ovaries. Scanning electron microscopy of mature ova (mean diameter 2.003 ± 0.01 mm) prominently showed the structure micropyle (mean diameter 12.93 ± 3.38 μm). Fecundity analyses suggested that September was the principal breeding season, whereas residual spawning occurred with fresh rain in late winter during February–March. Collectively, this is the first comprehensive qualitative and quantitative report of the seasonal variations in the ovarian development and function for S. plagiostomus. These data may provide valuable information towards the captive breeding programme as well as conservation and management for Schizothoracine fishes in normal and altered climatic conditions.

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