The diffusion transport coefficients of a binary granular suspension where one of the components is present in tracer concentration are determined from the (inelastic) Enskog kinetic equation. The effect of the interstitial gas on the solid particles is accounted for in the kinetic equation through two different terms: (i) a viscous drag force proportional to the particle velocity and (ii) stochastic Langevin-like term defined in terms of the background temperature. The transport coefficients are obtained as the solutions of a set of coupled linear integral equations recently derived for binary granular suspensions with arbitrary concentration [Gómez González et al., “Enskog kinetic theory for multicomponent granular suspensions,” Phys. Rev. E 101, 012904 (2020)]. To achieve analytical expressions for the diffusion coefficients, which can be sufficiently accurate for highly inelastic collisions and/or disparate values of the mass and diameter rations, the above integral equations are approximately solved by considering the so-called second Sonine approximation (two terms in the Sonine polynomial expansion of the distribution function). The theoretical results for the tracer diffusion coefficient D0 (coefficient connecting the mass flux with the gradient of density of tracer particles) are compared with those obtained by numerically solving the Enskog equation by means of the direct simulation Monte Carlo method. Although the first-Sonine approximation to D0 yields, in general, a good agreement with simulation results, we show that the second-Sonine approximation leads to an improvement over the first-Sonine correction, especially when the tracer particles are much lighter than the granular gas. The expressions derived here for the diffusion coefficients are also used for two different applications. First, the stability of the homogeneous steady state is discussed. Second, segregation induced by a thermal gradient is studied. As expected, the results show that the corresponding phase diagrams for segregation clearly differ from those found in previous works when the effect of gas phase on grains is neglected.