Resonance Raman Observation of Surface Carbonyl Groups on Carbon Electrodes Following Dinitrophenylhydrazine Derivatization

Fryling, Mark A.; Zhao, Jun; McCreery, Richard L.

doi:10.1021/ac00101a026

Cited by 48 publications

(53 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main reason is that the lowest potential applied during the CV was 0 V, thus avoiding the reduction of NO 2 into NHOH group as demonstrated before. Only a little, bad-defined reversible system was observed around 0.27 V on both grafted and unmodified electrodes and was assumed to belong to the quinone redox system often observed on GC after polarization [40][41][42]. The same behavior was obtained when the electrolysis at 0.3 V was performed in acetonitrile containing 2.5 mM NBD.…”

Section: Influence Of Grafting Potentialsupporting

confidence: 55%

New insight into 4-nitrobenzene diazonium reduction process: Evidence for a grafting step distinct from NO2 electrochemical reactivity

Richard

Evrard

Gros

2012

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

International audienceElectrochemical and spectroscopic investigations were performed in order to clarify the mechanism of 4-nitrobenzene diazonium reduction on glassy carbon in protic medium. The number and nature of the electron transfer processes were found to be strongly correlated to the electrode surface state. On polished electrode two different reduction peaks were observed. Selective electrolyses realized at the corresponding potential definitely proved that the grafting process actually occurs at a potential distinct from NO2 electroreduction, this latter inducing the presence of the quasi-reversible NO/NHOH redox couple at the electrode surface. These results were confirmed by XPS analyses. Furthermore, voltammetric experiments using Fe(CN)63- showed that the electrochemical properties of the modified electrode strongly depend on the potential applied for grafting, which modulates the nitro group oxidation state. All the results suggested that the electrode functionalization was more efficient when grafting and NO2 reduction were performed separately

show abstract

Section: Influence Of Grafting Potentialsupporting

confidence: 55%

New insight into 4-nitrobenzene diazonium reduction process: Evidence for a grafting step distinct from NO2 electrochemical reactivity

Richard

Evrard

Gros

2012

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Figure 4 shows the Raman spectra of (a) DNPH-oxiBSA and (b) BSA, and (c) the difference between (a) and (b), and (d) the Raman spectrum obtained for the protein-free DNPH solution. Evidently, DNPH and BSA conjugation, produces a marked change in the DNPH Raman spectra, mainly the red-shift of both the 1355 cm −1 peak (the dinitrophenyl-N bond stretch [36]), and the 848 cm −1 peak (ring breathing mode [37]). The major protein Raman features including the 1003 cm −1 phenylalanine peak and 1430 cm −1 methylene scissoring peak remains largely unchanged before and after DNPH conjugation.…”

Section: Resultsmentioning

confidence: 99%

Ratiometric Raman spectroscopy for quantification of protein oxidative damage

Zhang

Jiang

Yanney

et al. 2009

Analytical Biochemistry

View full text Add to dashboard Cite

A novel ratiometric Raman spectroscopic (RMRS) method has been developed for quantitative determination of protein carbonyl levels. Oxidized bovine serum albumin (BSA) and oxidized lysozyme were used as model proteins to demonstrate this method. The technique involves conjugation of protein carbonyls with dinitrophenyl hydrazine (DNPH), followed by drop coating deposition Raman spectral acquisition (DCDR). The RMRS method is easy to implement as it requires only one conjugation reaction, a single spectral acquisition, and does not require sample calibration. Characteristic peaks from both protein and DNPH moieties are obtained in a single spectral acquisition, allowing the protein carbonyl level to be calculated from the peak intensity ratio. Detection sensitivity for the RMRS method is ~0.33 pmol carbonyl/measurement. Fluorescence and/ or immunoassay based techniques only detect a signal from the labeling molecule and thus yield no structural or quantitative information for the modified protein while the RMRS technique provides for protein identification and protein carbonyl quantification in a single experiment.

show abstract

“…2. ), which has previously been shown by XPS to dramatically increase the number of carboxylic acid groups at the CNT surface [32,33]. It has also been recently demonstrated that a Ni electrode (oxidised to NiO(OH) at pH 10) is effective for NH 3 oxidation [15].…”

Section: Response To Ammoniamentioning

confidence: 87%

Electroanalytical Detection of n‐Butylamine at a Nickel/Carbon Nanotube Composite

Panchompoo¹,

Aldous²,

Liu³

et al. 2010

Electroanalysis

View full text Add to dashboard Cite

We report the electroanalytical detection of n-butylamine at a nickel/carbon nanotube (Ni/CNT) composite. Scanning Electron Microscopy (SEM) characterisation of the composite demonstrated that it consisted of bulk nickel particles ca. 2 mm in diameter entangled in CNT bundles. The spontaneous formation of Ni(OH) 2 was optimised, and comparison with a 3 mm nickel electrode showed that ca. 4 mg of the Ni/CNT composite cast on a 3 mm GC electrode possessed bulk nickel characteristics while also having higher activity and higher sensitivity towards the electrochemical detection of n-butylamine. However, the Ni/CNT composite showed no response to ammonia, in contrast to the macro-nickel-electrode.

show abstract

Resonance Raman Observation of Surface Carbonyl Groups on Carbon Electrodes Following Dinitrophenylhydrazine Derivatization

Abstract: Dinitrophenylhydrazine (DNPH) was used to form hydrazone derivatives of carbonyl groups on glassy carbon (GC) and pyrolytic graphite surfaces. The DNPH adducts of (10)

Cited by 48 publications

References 2 publications

New insight into 4-nitrobenzene diazonium reduction process: Evidence for a grafting step distinct from NO2 electrochemical reactivity

New insight into 4-nitrobenzene diazonium reduction process: Evidence for a grafting step distinct from NO2 electrochemical reactivity

Ratiometric Raman spectroscopy for quantification of protein oxidative damage

Electroanalytical Detection of n‐Butylamine at a Nickel/Carbon Nanotube Composite

Contact Info

Product

Resources

About