Resonance Raman scattering on haemoglobin

Brunner, H.; Sussner, H.

doi:10.1016/0005-2795(73)90004-4

Cited by 87 publications

(58 citation statements)

References 23 publications

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“…17 In follow-up work, Brunner used an oxygen isotope to identify a mode assignable to stretching of the iron-oxygen (Fe-O 2 ) bond at 567 cm À1 (Figure 2). 18 Understanding the nature of the Fe-O 2 bond was thought to be vital to the elucidation of oxygen binding in hemoglobin, and it became the subject of much research.…”

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confidence: 99%

“…38 Raman Spectroscopy Studies of Intact Red Blood Cells Intracellular Hemoglobin. In one of the early Brunner and Sussner studies mentioned above, 17 the investigators compared the spectrum of hemoglobin to the spectrum of intact RBCs and stated that ''the recorded Raman spectra of erythrocytes were identical to that of oxy-and deoxyHb, respectively, when we used the original suspension or added a small amount of deoxygenating dithionite''. 17 In 2001, almost thirty years after the original report, workers at Monash University in Victoria, Australia used conventional Raman spectroscopy (633 nm excitation) to record the same oxyHb (R state) and deoxyHb (T state) spectral features inside single living erythrocytes.…”

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Raman Spectroscopy of Blood and Blood Components

et al. 2017

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Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys. Measured levels of oxygen, nutrients, waste, and electrolytes in blood are often used for clinical assessment of human health. Raman spectroscopy is a nondestructive analytical technique that uses the inelastic scattering of light to provide information on chemical composition, and hence has a potential role in this clinical assessment process. Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. In this paper, we review the literature in the field, collate the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood, and attempt to draw general conclusions on the state of the field.

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Raman Spectroscopy of Blood and Blood Components

et al. 2017

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“…In terms of HbO 2 Sat determination, this method has the advantage that the oxygenation information is related only to the specific interaction of the photons with the Hb molecules, generating a unique Raman spectrum (6,7,12,36,40,41). The vibrational bands of heme are well known (1,5), and previous Raman investigations of Hb have been carried out on isolated and purified Hb or in isolated red blood cells (27,33,34).…”

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Hemoglobin oxygen saturation measurements using resonance Raman intravital microscopy

Filho

Terner

Pittman

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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A system is described for in vivo noninvasive measurements of hemoglobin oxygen saturation (HbO2Sat) at the microscopic level. The spectroscopic basis for the application is resonant Raman enhancement of Hb in the violet/ultraviolet region, allowing simultaneous identification of oxy- and deoxyhemoglobin with the same excitation wavelength. The heme vibrational bands are well known, but the technique has never been used to determine microvascular HbO2Sat in vivo. A diode laser light (power: 0.3 mW) was focused onto sample areas 15–30 μm in diameter. Raman spectra were obtained in backscattering geometry by using a microscope coupled to a spectrometer and a cooled detector. Calibration was performed in vitro by using glass capillaries containing blood at several Hb concentrations, equilibrated at various oxygen tensions. HbO2Sat was estimated using the Raman band intensities at 1,360 and 1,375 cm−1. Glass capillary path length and Hb concentration had no effect on HbO2Sat estimated from Raman spectra. In vivo observations were made in blood flowing in microvessels of the rat mesentery. The Hb Raman peaks observed in oxygenated and deoxygenated blood were consistent with earlier Raman studies that used Hb solutions and isolated cells. The method allowed HbO2Sat determinations in the whole range of arterioles, venules, and capillaries. Tissue transillumination allowed diameter and erythrocyte velocity measurements in the same vessels. Raman microspectroscopy offers distinct advantages over other currently used techniques by providing noninvasive and reliable in vivo determinations of HbO2Sat in thin tissues as well as in solid organs and tissues, which are unsuitable for techniques requiring transillumination.

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“…If the Raman excitation wavelength matches a transition wavelength of the molecule, a selective resonance enhancement of the porphyrin Raman signal occurs, without spectral interference from the surrounding protein. 37,38 A process within RBCs that is well-known to be sensitive to photo-dissociation is the binding of oxygen to Hb. 39 The Raman bands at 1640 cm À1 , 1582 cm À1 , and 1378 cm À1 are characteristic for the oxygenated form of human hemoglobin (oxyHb) and undergo a shift to 1604 cm À1 , 1553 cm À1 , and 1355 cm À1 upon transition to the deoxygenated form (deoxyHb).…”

Section: Rbc Diffusive Behavior and Membrane Edge Fluctuationsmentioning

confidence: 99%

A self-filling microfluidic device for noninvasive and time-resolved single red blood cell experiments

et al. 2016

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Existing approaches to red blood cell (RBC) experiments on the single-cell level usually rely on chemical or physical manipulations that often cause difficulties with preserving the RBC's integrity in a controlled microenvironment. Here, we introduce a straightforward, self-filling microfluidic device that autonomously separates and isolates single RBCs directly from unprocessed human blood samples and confines them in diffusion-controlled microchambers by solely exploiting their unique intrinsic properties. We were able to study the photo-induced oxygenation cycle of single functional RBCs by Raman microscopy without the limitations typically observed in optical tweezers based methods. Using bright-field microscopy, our noninvasive approach further enabled the time-resolved analysis of RBC flickering during the reversible shape evolution from the discocyte to the echinocyte morphology. Due to its specialized geometry, our device is particularly suited for studying the temporal behavior of single RBCs under precise control of their environment that will provide important insights into the RBC's biomedical and biophysical properties. Published by AIP Publishing. [http://dx

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Resonance Raman scattering on haemoglobin

Cited by 87 publications

References 23 publications

Raman Spectroscopy of Blood and Blood Components

Raman Spectroscopy of Blood and Blood Components

Hemoglobin oxygen saturation measurements using resonance Raman intravital microscopy

A self-filling microfluidic device for noninvasive and time-resolved single red blood cell experiments

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