Resonance Raman Studies of the Electronic State of Oxygen in Hemerythrin

Dunn, John B. R.; Shriver, Duward F.; Klotz, Irving M.

doi:10.1073/pnas.70.9.2582

Cited by 52 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another very simple example is given by oxygen: gaseous oxygen has a symmetric stretching frequency at 1555 cm" 1 and upon binding to the metal centres in the proteins haemocyanin and haemerythrin this oxygen vibration becomes RR enhanced (Section VI) and shifts to near 800 cm" 1 . This position is characteristic, on the basis of vibrational studies on various inorganic complexes, of oxygen in its peroxide O|~ state and strongly suggests that oxygen is liganded to haemerythrin and haemocyanin in this form (Dunn, Shriver & Klotz, 1973;Loehr, Freedman & Loehr, 1974).…”

Section: T H E Information Contained In Resonance Raman Spectramentioning

confidence: 92%

“…The spectral detail in this region is different for haemocyanins from mollusc and arthropod sources and suggests different coordination geometry about the Cu 2+ atoms for the two proteins. For oxyhaemerythrin excitation into an absorption feature in the 500 nm region produces an intensity enhanced 0-O stretch at 844 cm-1 , shifting to 798 cm" 1 upon 18 O 2 substitution (Dunn et al 1973). The frequency is again characteristic of the peroxide O|~ form.…”

Section: (A) Haemocyanin and Haemerythrinmentioning

confidence: 98%

See 1 more Smart Citation

Resonance Raman spectroscopy in biochemistry and biology

Carey

1978

Quart. Rev. Biophys.

View full text Add to dashboard Cite

Resonance Raman (RR) spectroscopy provides detailed information on the vibrational and electronic properties of biochemical and biological chromophores. The analysis of RR spectra, using for example model compounds or a group frequency approach, enables us to form an accurate structural picture of the chromophore in its natural biological site. Moreover, the insight gained into the electronic states of a biological chromophore can be crucial to our understanding of its function. Thus the RR technique represents a powerful means of eliciting precise structural and electronic data from a coloured species and of focusing upon key aspects of its function. It has even been possible to obtain RR spectra from some natural chromophores in vivo, giving spectra detailed and informative enough to please a spectroscopist from a system complex enough to satisfy a biologist.

show abstract

Section: T H E Information Contained In Resonance Raman Spectramentioning

confidence: 92%

Section: (A) Haemocyanin and Haemerythrinmentioning

confidence: 98%

Resonance Raman spectroscopy in biochemistry and biology

Carey

1978

Quart. Rev. Biophys.

View full text Add to dashboard Cite

show abstract

“…cumanense Hrs at pH 6.2 and pH 7.6. The Raman bands around 506-508 and 845 cm-l are assigned to the Fe-0 2 stretching ( v~~a~) and the 0-0 stretching (~00) modes, respectively (Kurtz et al, 1976(Kurtz et al, ,1977Dunn et al, 1973). The general features of RR spectra are similar between the cooperative and noncooperative Hrs, and also the structure of the binuclear center scarcely depends on the oligomeric size.…”

Section: While S Cumanensementioning

confidence: 95%

Resonance Raman study on the active-site structure of a cooperative hemerythrin

Kaminaka¹,

Takizawa²,

Handa³

et al. 1992

Biochemistry

View full text Add to dashboard Cite

Resonance Raman spectra were observed for the oxy and azidomet forms of a cooperative hemerythrin (Hr) isolated from Lingula unguis and a noncooperative Hr from Siphonosoma cumanense. The O-O stretching frequency of the oxy derivative of the L. unguis Hr was lower in the high-affinity form generated at pH 7.6 than in the low-affinity form generated at pH 6.2, while that of the S. cumanense Hr did not change at those two pH values. The Fe-O-Fe symmetric stretching mode of L. unguis azidomet-Hr exhibited a frequency shift between pH 7.6 and 6.2, while that of S. cumanense was not shifted. However, the corresponding band of the oxy form did not show a pH-dependent frequency change. Therefore, it is noted that the azidomet form is not a suitable model for studying a mechanism of cooperativity, contrary to the structural similarity between the oxy and azidomet forms. The Fe-O2 as well as Fe-N3 stretching frequencies were found to have no relation with the oxygen affinity. Upon exchange of solvent from H2O to D2O, the O-O and Fe-O2 stretching modes of L. unguis Hr were shifted to higher and lower frequencies, respectively, and their magnitudes were the same for the high- and low-affinity forms. The same frequency shifts were observed for S. cumanense Hr.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…SERS only enhanced the intensity of these bands, which means these bands are characteristics of the molecular structure of OxyHb. The tentative assignments of these bands are listed on Table . Only the aforementioned SERS bands will be discussed emphatically in terms of the change of OxyHb molecular vibration.…”

Section: Resultsmentioning

confidence: 99%

“…The SERS peak at 478 cm −1 because of the FeO stretching mode exhibits higher signal in diabetic group, suggesting that there is abnormal metabolism of heme in diabetic OxyHb. Specifically, the FeO stretching mode is sensitive to the change of oxidation environment . Compared with normal OxyHb, diabetic OxyHb is in the peroxidation environment with many active free radicals produced by abnormal metabolism .…”

Section: Resultsmentioning

confidence: 99%

Label‐free optical detection of type II diabetes based on surface‐enhanced Raman spectroscopy and multivariate analysis

Lin

Huang

Feng

et al. 2014

J Raman Spectroscopy

View full text Add to dashboard Cite

Surface‐enhanced Raman scattering (SERS) spectroscopy was first employed to detect oxyhemoglobin (OxyHb, the common type of hemoglobin) variation in type II diabetic development without using exogenous reagents. Using silver nanoparticles as SERS‐active substrate, high‐quality SERS spectra are obtained from blood OxyHb samples of 49 diabetic patients and 40 healthy volunteers. Tentative assignment of the observed SERS bands indicates specific structural changes of OxyHb molecule in diabetes, including heme transformation and globin variation. Furthermore, partial least squares and principal component analysis combined with linear discriminate analysis diagnostic algorithms are employed to analyze and classify the SERS spectra acquired from diabetic and healthy OxyHb, yielding the diagnostic accuracies of 90.0% and 95.5%, respectively. This exploratory work suggests that the silver nanoparticles‐based OxyHb SERS method in combination with multivariate statistical analysis has great potential for the label‐free detection of type II diabetes. Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

Resonance Raman Studies of the Electronic State of Oxygen in Hemerythrin

Abstract: Excitation of oxyhemerythrin with radia-

Cited by 52 publications

References 9 publications

Resonance Raman spectroscopy in biochemistry and biology

Resonance Raman spectroscopy in biochemistry and biology

Resonance Raman study on the active-site structure of a cooperative hemerythrin

Label‐free optical detection of type II diabetes based on surface‐enhanced Raman spectroscopy and multivariate analysis

Contact Info

Product

Resources

About