INTRODUCTIONPropolis or "bee glue" is a resinous material collected by honeybees from tree buds, sap flows or other botanical exudates and is used for sealing and coating bee hives. For many centuries, aside from its use as food, beverage and food supplement, it has long been used extensively in traditional medicine because of its wide range of therapeutic values for the treatment of common cold, flu, upper respiratory tract infections, as dermatological preparations for wounds, burns, acne, herpes simplex, genitalis and neurodermatitis, as mouthwash and toothpaste to prevent caries and gingivitis and for cosmetic applications [1,2]. Propolis was shown to exhibit a broad spectrum of biological activities which include antimicrobial [3,4], antifungal [5,6], free radical scavenging [7,8], anti-HIV [9,10], anti-inflammatory [11,12] and anti-herpes [13,14]. A recent study reported that propolis, at a concentration of 0.01 % (w/v), exhibited antiproliferative and cytotoxic actions against human fibroblasts proliferation in cell culture, followed by mild cell necrosis [15]. The dichloromethane crude extract and compounds, glyasperin A (1), propolin E (2) and propolin A (3), obtained from Philippine stingless bee (Tetragonula biroi Friese) nests, were evaluated for their cytotoxic potentials. The anti-proliferative activities of crude extract and compounds 1-3 against human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue ® cell viability assay. The crude extract was most anti-proliferative against HCT-116 cells (IC50 = 1.410 µg/mL). Glyasperin A (1) exhibited the strongest effect on MCF-7 cells (2.378 µg/mL). Propolin E (2) was most cytotoxic against HCT-116 cells (2.279 µg/mL), while propolin A (3) was most inhibitory against MCF-7 cells (2.815 µg/mL). Comparing the colorectal cancer cell lines, HCT-116 was generally more susceptible under crude extract, 2, and 3 (IC50 = 1.410, 2.279, 3.013 µg/mL, respectively) than 5.320, 5.359). Comparing the activities of propolin E and propolin A against HCT-116 cells, 2 (IC50 = 2.279 µg/mL) was more cytotoxic than 3 (3.013). The crude extract was more cytotoxic than Zeocin against HCT-116 cells. To our best of knowledge, this is the first report on the anti-proliferative properties of crude extract and compounds from T. biroi.