Resorcinol degradation by a Penicillium chrysogenum strain under osmotic stress: mono and binary substrate matrices with phenol

Guedes, Sumaya Ferreira; Mendes, Benilde Simões; Leitão, Ana Lúcia

doi:10.1007/s10532-010-9413-5

Cited by 11 publications

(6 citation statements)

References 47 publications

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“…The fungal strain was maintained at 4 °C on nutrient agar plates with 5.9% (w/v) NaCl. Precultures of cells were routinely aerobically cultivated in MC medium as described by [13] .…”

Section: Methodsmentioning

confidence: 99%

“…Cells were centrifuged for 10 min at 10,000 × g and washed three times in 0.85% (w/v) of NaCl. Then, a 10% aliquot was inoculated in MMFe medium (50 ml in 250-ml flasks) [13] with different concentrations of hydroquinone (Sigma–Aldrich, ReagentPlus™, ≥99%, Batch#: 114K2623) (see Section 3 ). Three replicates were used per test for each hydroquinone concentration.…”

Section: Methodsmentioning

confidence: 99%

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DNA damage induced by hydroquinone can be prevented by fungal detoxification

et al. 2014

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Hydroquinone is a benzene metabolite with a wide range of industrial applications, which has potential for widespread human exposure; however, the toxicity of hydroquinone on human cells remains unclear. The aims of this study are to investigate the cytotoxicity and genotoxicity of hydroquinone in human primary fibroblasts and human colon cancer cells (HCT116). Low doses of hydroquinone (227-454 μM) reduce the viability of fibroblasts and HCT116 cells, determined by resazurin conversion, and induce genotoxic damage (DNA strand breaks), as assessed by alkaline comet assays. Bioremediation may provide an excellent alternative to promote the degradation of hydroquinone, however few microorganisms are known that efficiently degrade it. Here we also investigate the capacity of a halotolerant fungus, Penicillium chrysogenum var. halophenolicum, to remove hydroquinone toxicity under hypersaline condition. The fungus is able to tolerate high concentrations of hydroquinone and can reverse these noxious effects via degradation of hydroquinone to completion, even when the initial concentration of this compound is as high as 7265 μM. Our findings reveal that P. chrysogenum var. halophenolicum efficiently degrade hydroquinone under hypersaline conditions, placing this fungus among the best candidates for the detoxification of habitats contaminated with this aromatic compound.

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“…The fungal strain was maintained at 4 °C on nutrient agar plates with 5.9% (w/v) NaCl. Precultures of cells were routinely aerobically cultivated in MC medium as described by [13] .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

DNA damage induced by hydroquinone can be prevented by fungal detoxification

et al. 2014

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“…We have previously characterized a halotolerant P. chrysogenum strain, P. chrysogenum var. halophenolicum , isolated from a salt mine (Leitão et al 2012 ), and able to metabolize phenolic compounds under osmotic stress (Leitão et al 2007 ; Guedes et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Penicillium chrysogenum var. halophenolicum on kraft lignin: color stabilization and cytotoxicity evaluation

et al. 2016

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Wood industries and agricultural crops generate an inexhaustible supply of by-products like lignin, which constitutes an environmental problem. Increasing efforts have been done to find new applications for lignin. One of them is as a food additive, but its chemical nature makes it sensitive to browning which constitutes a major drawback for this type of lignin application. In the present study we are documenting how color stabilization of a commercial kraft lignin was achieved after the treatment with Penicillium chrysogenum var. halophenolicum. In addition the fungal capacity to remove lignin is studied together with the effect of its treatment on cytotoxicity of lignin. P. chrysogenum var. halophenolicum was able to transform lignin, ensuring its color stability for more than 24 months. Dynamic light scattering and atomic force microscopy showed that the fungus contributed to homogenize particle size and hydrodynamic properties in lignin suspensions without increase the toxicity over HeLa cells and human primary fibroblasts. These findings suggest new uses for kraft lignin after P. chrysogenum var. halophenolicum treatment providing an effective approach for improve color stability.

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“…halophenolicum can metabolize up to 300 mg/L of phenol and resorcinol at 5.9% (w/v) of NaCl concentration. 16,17 However, the effects of phenolic compounds and sodium chloride on the genomic output of the penicillin biosynthesis cluster in Penicillium chrysogenum var. halophenolicum remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Effect of phenolic compounds and osmotic stress on the expression of penicillin biosynthetic genes from Penicillium chrysogenum var. halophenolicum strain

Guedes

Leitão

2012

J Xenobiotics

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Phenol and phenolic compounds are aromatic pollutants that inhibit biological treatment of wastewaters. Penicillium chrysogenum var. halophenolicum is a halotolerant fungus that previously showed the ability to degrade phenol and resorcinol in high salinity conditions. The presence of the penicillin biosynthetic cluster in P. chrysogenum var. halophenolicum was recently described. In this article, we examined the expression of pcbAB, pcbC and penDE, genes responsible for δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase, isopenicillin N synthase and isopenicillin N acyltransferase activities, respectively, in P. chrysogenum var. halophenolicum. A quantitative PCR (qPCR) approach was used to determine how these genes were expressed in media with 2% and 5.9% NaCl supplemented with phenol, catechol, hydroquinone and resorcinol as the sole carbon source. The effect of salt on the capability of P. chrysogenum var. halophenolicum to degrade aromatic compounds was measured using HPLC. qPCR analysis of RNA extracted from P. chrysogenum var. halophenolicum indicated that the expression levels of pcbAB, pcbC and penDE decreased in high saline concentrations compared to the levels expressed in media with glucose. High concentrations of salt significantly repress the expression of pcbAB and penDE. The pcbC gene was expressed differentially in catechol containing medium. There was no evident relationship between the expression levels of penicillin biosynthetic genes and yields of penicillin. Meanwhile, the presence of phenol and phenolic compounds seems to positively influence the antibiotic production; high concentrations of salt stimulated penicillin production. These results support the hypothesis that phenol, phenolic compounds and high concentrations of salt could act like a stress factor for P. chrysogenum var. halophenolicum resulting in higher yields of β-lactam antibiotic production.

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Resorcinol degradation by a Penicillium chrysogenum strain under osmotic stress: mono and binary substrate matrices with phenol

Cited by 11 publications

References 47 publications

DNA damage induced by hydroquinone can be prevented by fungal detoxification

DNA damage induced by hydroquinone can be prevented by fungal detoxification

Effects of Penicillium chrysogenum var. halophenolicum on kraft lignin: color stabilization and cytotoxicity evaluation

Effect of phenolic compounds and osmotic stress on the expression of penicillin biosynthetic genes from Penicillium chrysogenum var. halophenolicum strain

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