Resorculins: hybrid polyketide macrolides from <i>Streptomyces</i> sp. MST-91080

Chen, Rachel; Vuong, Daniel; Lacey, Ernest; Rutledge, Peter J.; Chooi, Yit‐Heng; Piggott, Andrew M.; Booth, Thomas

doi:10.1039/d2ob02332f

Cited by 4 publications

(5 citation statements)

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“…Next, we wanted to demonstrate the flexibility of getphylo to analyse other genetic elements. To demonstrate this, we reconstituted the evolutionary history of the resorculin BGC 15 (Case Study 2). getphylo successfully identified the conserved genes for 3,5-dihydroxybenzoic acid biosynthesis, in line with recently published results 15 .…”

Section: Resultsmentioning

confidence: 99%

“…To demonstrate this, we reconstituted the evolutionary history of the resorculin BGC 15 (Case Study 2). getphylo successfully identified the conserved genes for 3,5-dihydroxybenzoic acid biosynthesis, in line with recently published results 15 . This demonstrates getphylo's ability to build phylogeny for non-genome scale genetic elements, a function that will aid in the research of plasmids, phages and other gene clusters.…”

Section: Resultsmentioning

confidence: 99%

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getphylo: rapid and automatic generation of multi-locus phylogenetic trees

Booth

Shaw

Weber

2023

Preprint

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Phylogenetic trees are the primary tool for visualising evolutionary relationships. Traditionally, phylogenies are inferred from manually curated sets of marker genes. As available genomic data increases, there is increasing demand for tools to automatically build phylogeny from assembled genomes. Existing tools rely on reference databases of preselected marker genes, limiting their taxonomic scope. We sought to develop a tool that could quickly build phylogeny from input genomes alone. We developed getphylo, a tool to automatically generate multi-locus phylogenetic trees from GenBank files. It has a low barrier to entry with minimal dependencies. getphylo uses a parallelised, heuristic workflow to keep runtime and system requirements as low as possible. getphylo consistently produces trees with topologies comparable to other tools in at least half the time. Furthermore, as getphylo does not rely on reference databases, it has a virtually unlimited scope in terms of taxonomy (e.g., not limited to bacteria) and genetic scale (e.g., can analyse plasmids, prophage, and gene clusters). This combination of speed and flexibility makes getphylo a valuable addition to the phylogenetics toolkit.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

getphylo: rapid and automatic generation of multi-locus phylogenetic trees

Booth

Shaw

Weber

2023

Preprint

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“…MST-91080, is a 3,5-dihydroxybenzoic acid-derived macrolide. 16 Analysis of the resorculin biosynthetic gene cluster has revealed that it is a hybrid of type I and type II polyketide synthases (PKSs).…”

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confidence: 99%

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Hill

Sutherland

2023

Nat. Prod. Rep.

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“…New 14-membered macrolides derived from 3,5-dihydroxybenzoic acid, such as resorculin A 20 , have been discovered from Streptomyces species MST-91080. 18 Genome sequencing revealed that the resorculin biosynthetic gene cluster is a hybrid of type I and type III polyketide synthases (PKSs). Genome mining of Streptomyces cinnamoneus ATCC 21532 has identified a type I/type III PKS hybrid that generates macrolides, including cinnamomycin A 21 , from 3,5-dihydroxybenzoic acid.…”

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confidence: 99%

“…membered macrolides derived from 3,5-dihydroxybenzoic acid, such as resorculin A 20, have been discovered from Streptomyces species MST-91080 18. Genome sequencing revealed that the resorculin biosynthetic gene cluster is a hybrid of type I and type III polyketide synthases (PKSs).…”

mentioning

confidence: 99%