seeds contain oil in extractable quantities. Therefore, algae appear to be the cheapest source among all the renewable sources for biodiesel production. Green microalgae contain 20% to 70% lipid and exhibit extraordinary potential for cultivation as energy crops (Xu et al., 2006;de Vries et al., 2010). They do not need arable land for cultivation and can be grown in industrial, municipal, and agricultural effluents, and fresh-and seawater (Chinnasamy et al., 2010). The growth and oil producing efficiency of algae is much higher than that of conventional and oil seed crops such as corn and soybean (Li et al., 2008). Due to its high oil content, various countries like the UK, USA, China, Belgium, Denmark, India, and Singapore have started utilizing algae as a source of biofuels on a commercial scale.Cultivation modes and nutritional management affect the growth rate and biochemical composition of algae (Hsieh and Wu, 2009). Previous studies have demonstrated that the biomass and lipid content in some microalgae were affected by various cultivation conditions such as nitrogen supply and its sources (