Resources for Virus-Induced Gene Silencing in the Grasses

An Improved Brome mosaic virus Silencing Vector: Greater Insert Stability and More Extensive VIGS

“…necrosis, photobleaching, and stunting). Because the preparation of a VIGS construct and plant inoculation are inexpensive and not labor intensive, and the silencing results can be achieved in weeks, VIGS technology for gene function studies in dicotyledonous and monocotyledonous plants has advantages over stable RNAi transformation, which requires more reagents and labor and months to complete (Robertson, 2004;Scofield and Nelson, 2009;Senthil-Kumar and Mysore, 2011;Ramanna et al, 2013;Lee et al, 2015).…”

Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants

“…FoMV has been modified as an RNA i suppressordependent expression vector by deleting its coat protein (CP) and triple gene block genetic segments (Liu and Kearney, 2010). However, FoMV has not been successfully engineered as a VIGS vector, although it has been thought to be a potentially useful virus for VIGS in monocot hosts (Scofield and Nelson, 2009).…”

SILENCING OF G-PROTEIN -SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH

“…Virus-induced gene silencing (VIGS), on the other hand, is a useful and efficient approach for plant loss-of-function evaluation that can overcome the above-mentioned complications and permits knockdown of genes-of-interest and phenotypic observations within 3 to 4 weeks. VIGS allow stable RNA interference (RNAi) in plant cells and can also be performed in the species that are difficult to transform (Burch-Smith et al, 2004;Scofield and Nelson, 2009;Cakir et al, 2010). Recently, the plants in which VIGS has been used were increased considerably, and more than 30 viruses have been reported to have great prospective as VIGS vectors (Yuan et al, 2011).…”