Respiration and aerobic glycolysis in rat cerebral cortex during postnatal maturation. Influence of hypothyroidism

Ghittoni, Nora E.; Gómez, César Cinesi

doi:10.1016/0024-3205(64)90108-0

Cited by 12 publications

(3 citation statements)

References 10 publications

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CONCOMITANTLY with post-natal development of the neuropil and with the appearance of myelin, a progressive increase in the concentration of several free amino acids has been found in normal developing brain of rats, cats, and rabbits ( SCHADE and BAXTER, 1960;VERNADAKIS and WOODBURY, 1962; BERL and PURPURA, 1963 ; MIHAILOVIC and KOZALIC, 1964).Neonatal thyroidectomy in rats produces several morphological alterations in the brain (BARNETT, 1943; EAYRS, I961a; LEGRAND, KRIEGEL and JOST, 1961) coincident with changes in the spontaneous electrical activity (BRADLEY, EAYRS and SCHMALBACH, 1960) and a decrease in the activity of certain enzymes (HAMBURGH and FLEXNER, 1957) and in the oxidative metabolism of glucose in cerebral cortex (GHITTONI and GOMEZ, 1964).The present work was undertaken to find out the influence of neonatal thyroid deprivation upon the changes occurring in free amino acids of brain during post-natal development. The results indicate that thyroid deficiency at birth leads to a transitory depression of the concentration of glutamic acid, glutamine, and GABA, and to permanent changes in the levels of aspartic acid and taurine in the brain.

METHODSRats of an inbred Wistar strain were used.

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mentioning

confidence: 99%

“…Neonatal thyroidectomy in rats produces several morphological alterations in the brain (BARNETT, 1943; EAYRS, I961a; LEGRAND, KRIEGEL and JOST, 1961) coincident with changes in the spontaneous electrical activity (BRADLEY, EAYRS and SCHMALBACH, 1960) and a decrease in the activity of certain enzymes (HAMBURGH and FLEXNER, 1957) and in the oxidative metabolism of glucose in cerebral cortex (GHITTONI and GOMEZ, 1964).…”

mentioning

confidence: 99%

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Influence of Neonatal Hypothyroidism on Amino Acids in Developing Rat Brain

Guglielmone

Gómez

1966

Journal of Neurochemistry

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METHODSRats of an inbred Wistar strain were used.

…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Influence of Neonatal Hypothyroidism on Amino Acids in Developing Rat Brain

Guglielmone

Gómez

1966

Journal of Neurochemistry

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“…Impaired neuronal matura tion and synaptogenesis are reflected in neu ropil hypoplasia and decreased numbers of synaptic profiles in cerebral and cerebellar cortex [6,15,16,34]. The primary cause(s) of this retarded development, however, has not been determined.Biochemical studies of the developing brain during neonatal hypothyroidism have sug gested that an alteration of brain mitochondria may occur in this condition [18][19][20]24,37,51]. Abnormal development of brain mitochondria could result in reduced energy equivalents being available for anabolism, thereby con tributing to retarded synaptogenesis.…”

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Membrane Enzyme Development in Nerve Ending Mitochondria during Neonatal Hypothyroidism

Battie¹,

Verity²

1979

Dev Neurosci

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The effect of propylthiouracil-induced neonatal hypothyroidism on the development of synaptosome fraction mitochondrial membrane enzymes has been determined. Total and specific activities of two mitochondrial inner membrane (cytochrome c oxidase and succinate cytochrome c reductase) and two outer membrane enzymes (monoamine oxidase and rotenone-insensitive NADH cytochrome c reductase) were determined. The inner mitochondrial membrane enzymes developed in synchrony in euthyroid animals, but a decrease in fractional and specific activities of both inner membrane enzymes occurred in hypothyroidism. The outer membrane enzymes developed asynchronously; monoamine oxidase revealed a 9-fold increase while NADH cytochrome c reductase increased 4-fold. Hypothyroidism caused no change in total fractional activity or K_m and an increased specific activity of outer membrane enzymes.

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Effects of neonatal hypothyroidism on cerebral and cerebellar synaptosome development

Verity

Brown

Cheung

et al. 1976

J of Neuroscience Research

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The effect of hypothyroidism on cerebral and cerebellar synaptosome development has been studied. Neonatal hypothyroidism was induced following addition of 0.3% propylthiouracil to the diet of nursing mothers. Maturation profiles of total synaptosome fraction and specific activities of lactate dehydrogenase, Na+K ATPase, cytochrome c oxidase, and protein were obtained from days 6 to 32 on synaptosomes isolated from Ficoll-sucrose gradients. The greatest changes were found in the total activities of enzymes isolated from the cerebellum. Hypothyroidism induced a retardation of LDH and cytochrome c oxidase in cerebellar synaptosomes, but no change in corresponding specific activities. Maximum rates of 14C-leucine incorporation into cerebellar synaptosome protein was found at 16-20 days, after which a rapid decline occurred to adult levels at 32 days. In neonatal hypothyroidism, synthesis was significantly reduced at 8 and 14 days, but reached control levels or above at 21--32 days. In the cerebrum, maximum rates of 14C-leucine incorporation into synaptosome protein were identified at 8--12 days in normal with a rapid drop to adult levels at approximately 20 days. In neonatal hypothyroidism, peak activities were identified at 14 days and increased activities over control were noted at 14, 20 and 30 days. These observations demonstrate the sensitivity of the developing cerebellar synaptic apparatus to neonatal hypothyroidism, with a protraction in the peak levels of synaptosome protein synthesis in cerebrum and cerebellum.

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Respiration and aerobic glycolysis in rat cerebral cortex during postnatal maturation. Influence of hypothyroidism

Cited by 12 publications

References 10 publications

Influence of Neonatal Hypothyroidism on Amino Acids in Developing Rat Brain

Influence of Neonatal Hypothyroidism on Amino Acids in Developing Rat Brain

Membrane Enzyme Development in Nerve Ending Mitochondria during Neonatal Hypothyroidism

Effects of neonatal hypothyroidism on cerebral and cerebellar synaptosome development

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