Respiration of Virus–infected Plant Tissue and Effect of Light on Virus Multiplication

The Effect of Light on Tobacco Mosaic Virus (TMV) Formation

“…Thus for the young tissue used in this work enzyme treatment is unnecessary to extract all the virus. Similar results were obtained by Takahashi (1951).…”

“…In darkness the formation of TMV depends on respiratory activity (Takahashi 1948, Tien and Tang 1963, Sasaki and Hirai 1963, and in the light it is probably connected with photosynthesis (Goffeau andBove 1965, Doke andHirai 1970). However, in experiments on the multiplication of TMV in discs punched from inoculated tobacco leaves, some batches of continuously illuminated discs formed TMV even when yellow and senescent.…”

“…Discs kept dark for 48 hours and then placed in the light, formed about half as much virus as those continuously illuminated whereas discs illuminated for 48 hours and then placed in the dark formed as much virus during the next 48 hours as those kept in the light. This observation was analysed further because other workers have found that less virus is formed in the dark than in the light (Holmes 1934, Takahashi 1947, Pound and Bancroft 1956, Kassanis 1953.…”

Untersuchungen über den Stoffwechsel viruskranker Pflanzen.

“…Fiir die Lichtmangelversuche wurden die Pflanzen entweder im Gewachshaus oder in einem besonderen Kellerraum mit relativ konstanten Licht-, Temperatur-und gen in der Literatur vor (BEST 1936, TAKAHASHI 1947, BA>X^DEN und ROBERTS 1948, WATSON 1950, 1955. Geringe Lichtintensitaten oder Dunkelheit nach Infektion der Wirtspflanzen reduzieren die Lasionenzahl auch bei verschiedenen anderen Virus-Wirt-Kombinationen (BAWDEN und ROBERTS 1948, WILKINSON und BLODGETT 1948, LINDNER, KIRKPATRICK und WEEKS 1959, FiuGUELET und HOOKER 1966.…”

Some Relations of Carbohydrate Level of the Host to Plant Virus Infections

“…dusted on their upper surfaces with carborundum as described by Rawlins and Tompkins (1936), and then brushed as described by Takahashi (1947) with a water suspension of virus in I per cent K2HP04• The virus-water suspension was prepared by grinding in a mortar a small measured piece of an infected leaf (usually about I sq. em., or 0.03 g. with about 2 drops of water and then diluting as desired.…”