Respiratory allergies with no associated food allergy disrupt oral mucosa integrity

Sánchez-Solares, Javier; Delgado-Dolset, María Isabel; Mera-Berriatua, Leticia; Hormias-Martin, Gonzalo; Cumplido, José; Saiz, Vanesa Bautista; Carrillo, Teresa; Moreno‐Aguilar, Carmen; Escribese, María M.; Gómez‐Casado, Cristina; Barber, Domingo

doi:10.1111/all.13860

Cited by 15 publications

(41 citation statements)

References 10 publications

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“…The mucosa immune system regulates the entrance of multiple antigens either pathogenic or nonpathogenic, such as food and pollen allergens. Interestingly, it has been described that in spite of the absence of food allergy, the oral mucosa could be compromised due to a respiratory sensitization . A disrupted barrier could be responsible for food allergy development, which support our hypothesis about the sensitization process in some analyzed patients.…”

Section: Discussionsupporting

confidence: 88%

New insights into the sensitization to nonspecific lipid transfer proteins from pollen and food: New role of allergen Ole e 7

Oeo-Santos

Navas

Benedé

et al. 2019

Allergy

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Background Ole e 7 is a nonspecific lipid transfer protein (nsLTP) from olive pollen, one of the main allergenic pollens worldwide. This allergenic nsLTP is responsible for severe symptoms in regions with high olive pollen exposure, where many Ole e 7‐sensitized patients exhibit a co‐sensitization to the peach nsLTP, Pru p 3. However, there is no evidence of cross‐reactivity, which explains this observed co‐sensitization. Therefore, the purpose of this study was to explore the relationship between Ole e 7 and Pru p 3. Methods A total of 48 patients sensitized to Ole e 7 and/or Pru p 3 were included in the study. Specific IgE serum levels were measured by ImmunoCAP 250 and ELISA. Inhibition assays were performed to determine the existence of cross‐reactivity between both nsLTPs. Allergic response was analyzed ex vivo (basophil activation test) and in vitro (RBL‐2H3 mast cell model). Results Common IgG and IgE epitopes were identified between both allergens. IgE‐binding inhibition was detected in Ole e 7–monosensitized patients using rPru p 3 as inhibitor, reaching inhibition values of 25 and 100%. Ex vivo and in vitro assays revealed a response against rPru p 3 in four (31%) Ole e 7–monosensitized patients. Conclusions Our results suggest that Ole e 7 could play a new role as primary sensitizer in regions with high olive pollen exposure, leading to the peach nsLTP sensitization. This co‐sensitization process would occur because of the cross‐reactivity between Ole e 7 and Pru p 3 observed in some allergic patients.

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Section: Discussionsupporting

confidence: 88%

New insights into the sensitization to nonspecific lipid transfer proteins from pollen and food: New role of allergen Ole e 7

Oeo-Santos

Navas

Benedé

et al. 2019

Allergy

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“…the lower the E-cadherin expression, the more the Treg numbers in the buccal mucosa). Thus, we hypothesize that the Fox p3+ cells we observed in CD patients are recruited to protect against further tissue damage and maintain barrier integrity, as previously reported (28,41). In fact, we found a positive correlation between Fox p3+ cell numbers in the oral mucosa and peripheral amphiregulin expression, which is a repair factor.…”

Section: Discussionsupporting

confidence: 86%

“…Previous studies by our group with allergic patients to grass pollen, olive pollen, or house dust mite (HDM) have demonstrated that they undergo oral epithelial remodeling characterized by reduced expression of claudin-1, occludin, and E-cadherin proteins ( 25 , 41 ). In fact, severe grass pollen allergic patients also presented an increased number of CD11c+ and CD4+ infiltrates and increased gene expression of IL-33 ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Celiac Disease Causes Epithelial Disruption and Regulatory T Cell Recruitment in the Oral Mucosa

et al. 2021

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Celiac disease (CD) is a chronic autoimmune disease characterized by an immune-triggered enteropathy upon gluten intake. The only current treatment available is lifelong Gluten Free Diet (GFD). Several extraintestinal manifestations have been described in CD, some affecting the oral mucosa. Thus, we hypothesized that oral mucosa could potentially be a target for novel biomarkers and an administration route for CD treatment. Six de novo diagnosed and seven CD patients under GFD for at least 1 year were recruited. Non-celiac subjects (n = 8) were recruited as control group. Two biopsies of the cheek lining were taken from each subject for mRNA analysis and immunohistochemical characterization. We observed a significant decrease in the expression of epithelial junction proteins in all CD patients, indicating that oral mucosa barrier integrity is compromised. FoxP3+ population was greatly increased in CD patients, suggesting that Tregs are recruited to the damaged mucosa, even after avoidance of gluten. Amphiregulin mRNA levels from Peripheral Blood Mononuclear Cells (PBMCs) and epithelial damage in the oral mucosa correlated with Treg infiltration in all the experimental groups, suggesting that recruited Tregs might display a “repair” phenotype. Based on these results, we propose that oral mucosa is altered in CD and, as such, might have diagnostic potential. Furthermore, due to its tolerogenic nature, it could be an important target for oral immunotherapy.

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“…However, these patients do not present inflammatory infiltrates in the oral mucosa, probably due to the lack of oral antigen exposure. Altogether, these data suggested that in severe respiratory allergic phenotypes, there is a systemic exacerbated inflammatory response underlying the disruption of the oral mucosa integrity [46].…”

Section: Novel Mechanisms Associated With Severe Allergic Phenotypesmentioning

confidence: 99%

Metabolomics strategies to discover new biomarkers associated to severe allergic phenotypes

Barber

Villaseñor

Escribese

2019

Asia Pacific Allergy

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In the last decades have emerged new technological platforms that allow evaluation of genes, transcripts, proteins, or metabolites of a living being, so-called omics sciences. More importantly, new technics for their integration have provided access to a complete set of information of the current conditions and features of a specific biological sample in a precise moment. Thus, omic sciences are now considered an essential tool for patient stratification in base to their severity, to understand disease progression and to identify new biomarkers. Severe patients, that are out of control, provide an excellent model to understand disease evolution and to identify new intervention and biomarkers strategies. Here we discuss the use of metabolomics to understand severity in allergic diseases in a strategy that opens new insights as well as identify new biological systems relevant for allergy progression. Metabolomics strategies are based in parallel evaluation of different allergy severity models by mean of untargeted analysis that allows the identification of potential biomarkers. Overlapping of different biomarkers in multiple models, provides information of general as well as specific biological systems involved in each model. Later a selected panel of biomarkers will be used in a target method to explore the diagnosis potential to stratify allergic patients.

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Respiratory allergies with no associated food allergy disrupt oral mucosa integrity

Cited by 15 publications

References 10 publications

New insights into the sensitization to nonspecific lipid transfer proteins from pollen and food: New role of allergen Ole e 7

New insights into the sensitization to nonspecific lipid transfer proteins from pollen and food: New role of allergen Ole e 7

Celiac Disease Causes Epithelial Disruption and Regulatory T Cell Recruitment in the Oral Mucosa

Metabolomics strategies to discover new biomarkers associated to severe allergic phenotypes

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